ASSOCIATION STUDY BETWEEN ANGIOTENSIN II TYPE 2 RECEPTOR (T1247G and A5235G) POLYMORPHISMS AND BREAST CANCER AMONG EGYPTIAN FEMALES

Background: Breast cancer is the most common type of cancer among women. Many types of cancer are associated with polymorphisms of the renin-angiotensin system (RAS). Angiotensin II (Ang II) is a vasoconstrictor, a mitogen and an angiogenic factor. Ang II exerts its effect through two receptors (AGTR 1-2). T1247G and A5235G are two SNPs within the 5′-region of the AGTR2 gene. Objectives: Our aim was to investigate the association between (T1247G and A5235G) SNPs with breast cancer among Egyptian females. Patients and Methods: One hundred Egyptian women were included in the present study; 50 cases and 50 controls. Data about age, menstrual and family history were obtained. TNM category and axially LN status were also assessed in breast cancer patients. All subjects were genotyped for AGTR2 (T1247G and A5235G) SNPs using the Custom TaqMan SNP Genotyping assays. Results: For A5235G, women carrying genotypes AA/AG were more likely to develop breast cancer than GG carriers (OR = 4.125, 95% CI: 1.473-11.555). A significant association was observed between (A5235G) genotypes and both TNM category and axillary LN. Also, a significant association between (T1247G) genotypes and TNM category with the mutant GG genotype being predictor of poor outcome. Multivariate logistic regression analysis proved that (A5235G) SNP genotypes significantly contribute to the prediction of breast cancer risk. Conclusions: Our study suggests that RAS is implicated in the development and in the invasion of breast cancer.