31Jan 2016

Development and validation of method for molecular weight determination of cellulose using GPC column in HPLC.

  • Analytical Science & Technology, Aditya Birla Science and Technology Company Private Ltd, Plot no. 1 & 1-A1, MIDC, Taloja, District Raigad, Maharashtra, 410206, India.
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The longer analysis time required to estimate the molecular weight on traditional GPC. The purpose of this study is to establish method for molecular weight analysis of cellulose using lesser number of GPC columns, shorter analysis time and lesser cost of analysis using HPLC in GPC mode. The method is validated to cover system precision, linearity, accuracy, method precision, robustness and solution stability to support the study. Pullulan polysaccharide is used as standard and 0.5% LiCl in N,N-dimethylacetamide as eluent. The comparative data of four columns versus two columns and HPLC versus traditional GPC is represented in the results and discussion section.


[Satish N. Patkar and Prasad D. Panzade (2016); Development and validation of method for molecular weight determination of cellulose using GPC column in HPLC. Int. J. of Adv. Res. 4 (Jan). 516-530] (ISSN 2320-5407). www.journalijar.com


Prasad D. Panzade