Isolation, identification and characterization of the biologically active compound in ethyl acetate extract of Sansevieria zeylanica and evaluation of its antimicrobial effect against Klebsiella pneumoniae

The present study was aimed to isolate and identify the active compound in ethyl acetate extract of Sansevieria zeylanica and evaluation of its antimicrobial effect against Klebsiella pneumoniae. Dried powdered plant material was used in this study, extracted with ethyl acetate using soxhlet apparatus. Column chromatography was used for the separation and purification of active compounds in ethyl acetate extract of Sansevieria zeylanica. FT IR spectrum, HNMR spectrum,13CNMR spectrum analysis were used for elucidating the structure of isolated compounds of Sansevieria zeylanica. The phytochemical analysis of the compound was done by using standard procedure. The anti microbial effect of the active compound was evaluated by disc diffusion method. Phytochemical analysis of compound isolated from ethyl acetate extract of Sansevieria zeylanica shows the presence of phenolic compounds. Analysed peaks of FT IR spectrum, HNMR spectrum,13CNMR strongly suggested that the experimental compound may be a phenyl-2-hydroxy decyl ketone. The antimicrobial assay of phenyl-2-hydroxy decyl ketone confirms the efficacy of the compound as anti bacterial agent and also used as drug for treating various disease associated to Klebsiella pneumoniae.