Therapeutic effects of curcumin and royal jelly as natural antioxidants on some biochemical parameters in hepatotoxicity induced by carbon tetrachloride (CCl4) in male albino rats

The present study was designed to determine the possible therapeutic effects of curcumin, royal jelly and both against carbon tetrachloride (CCl4) induced oxidative stress and biochemical changes in liver of albino rats. A patch of 135 male Wister albino rats averaged weights (190±10 g) at the beginning of the experiment were divided into 9 main groups according to the treatment and requirements of the experiment. The rats injected i.p. with CCl4 at dose (2 mL/kg, 1:1 in olive oil) twice per week and received, via gavage, curcumin at dose (150 mg/kg b.wt), royal jelly at dose (500 mg/kg b.wt) and the combined supplementation of both curcumin (150 mg/kg b.wt) and royal jelly (500 mg/kg b.wt) along the experimental period. Each group contains 15 rats and five rats were sacrificed on the 2nd, and 4th and 6th week from each group. The results refer to a significant elevation of some hepatic parameters (TBARS, ASAT, ALAT, and ALP) while a significant decrease in some other parameters (Total protein, albumin, CAT, GSH and SOD). on the 2nd ,4th and 6th week in rats injected intraperitoneal with CCl4 as compared to the control groups. The administration of the curcumin, royal jelly or both had beneficial and decrease side effects against the deleterious changes of CCl4. In conclusion, according to the results obtained the administration of the curcumin and royal jelly or both provides considerable hepatoprotective and hepatotherapeutic effects against intoxicated with CCl4 in male Wister albino rats by preventing oxidative stress through ROS scavenger and improvement in the former biochemical parameters.