Association between HCMV and Some Procoaculations Factors in Aborted Women in AL- Najaf Province, Iraq

This study has been conducted on seventy three patient's women suffering from abortion who have been checked Central Laboratory of Al-Hakeem Hospital in Al-Najaf city from November 2013 to April 2014. Their ages range from (14-44) years old .In addition, (20) healthy adults women, without any history of abortion or inflammatory diseases as control group. Blood samples have been withdrawn from abortion women a volume of 5 milliliters of venous blood, 0.9 milliliters have been anticoagulated with 0.1 milliliters sodium citrate and centrifuged at 3000 r.p.m for 15 minutes. The plasma has been aspirated to another test tube for storage in deep freeze (-20?C) until analysis for precoagulate V factor, whereas serum sample was obtained from the rest of blood and stored at -20°C until processing. Serum concentration of anti-HCMV-IgG was measured by means of Enzyme Link Fluorescent Assay technique (ELFA). Serum concentration of procoagulant factor X activator (FXa) and factor V (F5) were measured through ELISA technique. The results have shown a significant relations of (P<0.0001) among anti-HCMV IgG antibody in the groups of patients women suffering from abortion compared to those of the healthy Control Group. HCMV-IgG is correlated positively with factor V (F5) (p< 0.0001), while CMV-IgG has shown a negative correlation with factor Xa (FXa) (P< 0.0001). It has been concluded that the serum level of anti HCMV-IgG antibody plays a major role in the diagnosis of patient with HCMV-aborted women also the changes in level of both factor V and factor Xa plays a major role in cases of abortion.