Extraction and Identification of some metabolites produced by antagonistic apple plant bacteria Brevibacterium halotolerans
- Department of Microbiology and Plant Physiology, Faculty of Biology, Saratov State University, 83 Astrakhanskaya Street, 410012 Saratov, Russia.
- Institute of Pharmaceutical Biology and Biotechnology, Heinrich-Heine University, 40225 Duesseldorf, Germany.
- Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, 35516 Mansoura, Egypt.
- Institute of Medical Microbiology and Hospital Hygiene, University Clinic of the Heinrich-Heine University, 40225 Duesseldorf, Germany.
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Many microorganisms are constantly selected by researchers for their antagonistic activity in in vitro or in plant screening systems Brevibacterium halotolerans strain have antagonistic activity against bacterial and fungal pathogens was isolated from 16 different apple plant stem samples from Golden delicious apple fields at Volga region, Saratov city, Russia. Bacterial secondary metabolites are believed to represent an important tool in chemical interaction and communication. Chemical metabolite analyses of B. Halotolerans indicated that all identified metabolites were likely responsible for the antagonistic activity and indicates that bioactivity may play an ecological role in these habitats. This isolate (SA87) morphologically and Molecular characterization performed by 16S rRNA gene sequence analysis and it was confirmed as Brevibacterium halotolerans. Among the secreted extract seven components, named Neurolenin B (1), Cyclopenin (2), Auronitol (3), Palitantin (4), Malformin A (5), Pestalotiopisorin A (6) and 2-hydroxy-3-methylbenzoic acid (7) were isolated from apple associated endophytic bacteria (SA87) cultivated in solid rice medium. Bacterial metabolites and their structures were elucidated by spectroscopic methods, including High performance liquid chromatography HPLC. And Liquid chromatography–mass spectrometry LC-MS.
[Hassan A. Ahmed, Weaam Ebrahim, Peterson A. Mikhailovna, Birgit Henrich, Peter Proksch (2015); Extraction and Identification of some metabolites produced by antagonistic apple plant bacteria Brevibacterium halotolerans Int. J. of Adv. Res. 3 (Oct). 1208-1217] (ISSN 2320-5407). www.journalijar.com
