The interferon –? level associated with human adenovirus detected in urine of Hemorrhagic cystitis patients.

Two hundred bloody urine and 200 blood specimens were collected from200 Patients selected to eliminate other causes of hematuria;(149 male and 51 female, age 4- 46 years) all patients suffering from hemorrhagic cystitis(HC) attending to some of hospitals in Babylon governorate over period between April 2014 to December 2014. Detection of Adenovirus was done by direct chromatography technique by using manual principle of CreTest company as a rapid detection and Direct Immunoflouresecnt Technique according to manual procedure of Vircell company to evaluation of the interferon ? among a group of patients infected by Adenovirus. The quantitative determination of human IFN-? concentrations in serum by uses Sandwich-ELISA as the method. This idea refer to the uses the concentration of interferon ? as the monitor to the immune status for patients suffering hemorrhagic cystitis. The age range distribution showed that the greatest patients group at age 1- 10 y, 21 (31 %) . The present study as shown the male percentage53(78%) was significantly higher than female 15(22%) at p value ( < 0.05) .And show the 9(13%) positive result of Adenovirus by IC, DIF, and IDIF technique. The result showed that higher significant level ( IFN-? ) at comparison with control samples at P value (< 0.05) of T test. Gender relationship with IFN-? showed the female patients have higher level than male of IFN-?. This study shows detected Adenoviruses in bloody urine by rapid chromatographic test, so this method could be applicable in hospitals laboratories and using of certain immunological factors such as (INF-? ) to monitor the effectiveness of such virus on the patients at acute and chronic state .