The impact of mushroom on elusive medical biological activities

This study aimed to investigate the possible biological activity of aqueous, methanolic and sulfated extracts of tow fungi (Mushrooms) the white mushroom (Agaricus bisporus) and the oyster mushroom (Pleurotus florida) at 85 °C under acidic, neutral and alkaline conditions. Fibrinolytic, anticoagulation, antioxidant, antitumor and antimicrobial activities were investigated. The Biochemical studies of the chosen two fungi showed the presence of varying amounts and different forms of total ash, protein, lipid, and total carbohydrates. However, Paper chromatography examination showed that the output of the acid hydrolysis to the plants varying from monosaccharaides. Anticoagulation and fibrinolytic properties of the crude extracts as well as of their sulfated derivatives, at 2 mg/ml of extract were tested and show that the acidic extract, sulfated acidic extract, sulfated neutral and sulfated alkaline extract of A. bisporus and P. florida amazingly exhibited fibrinolytic activities equivalent to double of the same amount of standard "Hemoclar". On the other hand, results also showed a promising anticoagulant activities of the sulphated extracts at different concentrations compared with the corresponding native extracts. Also, their antimicrobial activity of the aqueous extracts against both tested Gram positive and negative bacteria, yeast and fungal strains was determined. The chemical modification of the aqueous sulfation can increase significantly its anticoagulation and fibrinolytic activities. And also their extracts show antioxidant activity reached up to 70% comparative by standard. However, the antitumor activity is high for both the native and the sulfated aqueous extracts.