Effect of widely used industrial chemicals on cell signaling pathways and cytoskeletal integrity in mammalian system
- UGC-DAE-Consortium for Scientific Research, Kolkata Centre, 3/LB-8, Bidhannagar, Kolkata-700098.
- Department of Environmental Science, University of Calcutta, Kolkata-700019.
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The paper described effects of 4-Dimethylaminoazobenzene (p-DAB) and Diethylnitrosamine (DENA), two well known hepatotoxicants, on cytoskeletal dynamics of hepatocytes and associated signaling pathways. Swiss albino mice exposed to p-DAB (165mg/kg bw/mouse/day, orally) and DENA (Single dose, i.p 200mg/kg bw) for six weeks manifested drastic distortion of microfilament and microtubules architecture in the hepatocytes in addition to remarkable increase in intracellular ROS generation. p-DAB and DENA induced dysregulation of signaling pathways involving Rho-GTPases was reflected in significant downregulation of expressions of RhoA, Rac1 and Cdc42 proteins. Data showed considerable changes in expression of regulatory kinases viz. LIMK1, LIMK2 and SAPK/JNK, and also highlighted the crosstalk between C-Raf and proapoptotic kinase MST2. The present study reported for the first time the potential of p-DAB and DENA to alter Rho signaling protein expressions and disrupt cytoskeletal architecture via involvement of intracellular free radicals. Our data provided a cue to consider such changes to be important benchmark for assessing carcinogenic prospective of p-DAB and DENA.
[Dipanwita Mukherjee, Aniruddha Mukhopadhyay and Anindita Chakraborty (2015); Effect of widely used industrial chemicals on cell signaling pathways and cytoskeletal integrity in mammalian system Int. J. of Adv. Res. 3 (Sep). 408-419] (ISSN 2320-5407). www.journalijar.com
