Diversity of Actinomycetes from fodder leguminous plants and their biocontrol potential
- Department of Microbiology, Punjab Agricultural University, Ludhiana-141004, Punjab, India.
- Electron Microscope and Nanoscience Laboratory, Punjab Agricultural University, Ludhiana-141004, Punjab, India.
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A total of 94 actinomycete isolates were obtained from 20 samples of rhizospheric soil and 20 samples of roots of fodder leguminous plants viz: Vigna unguiculata and Trifolium alexandrinum. Actinomycetes were more frequently recovered from rhizospheric soil (64% of all isolates) followed by roots (18%) and nodules (18%) respectively. The highest number and diversity of actinomycetes were isolated from T. alexandrinum rhizospheric soil (4.2×105 c.f.u/g). Forty one out of 94 isolates exhibited antagonistic activity against at least one of the three phytopathogenic fungi tested viz: Fusarium oxysporum, Fusarium moniliforme and Sclerotinia sclerotiorum. Scanning electron microscopy revealed rupture of the F. moniliforme mycelial cell wall at the area of interaction with CS16. It was observed that genus Streptomyces was dominant (63%) followed by Micromonospora (15%), Nocardia (10%), Saccharopolyspora (7%) and Actinopolyspora (5%). The isolates CS1 and CS44 were found to be promising in terms of seed germination and wilt control in cowpea crop under green house conditions. Therefore these isolates may be used as potential biocontrol agents against Fusarium wilt caused by Fusarium oxysporum.
[Harpreet Kaur, Madhurama Gangwar and Anu Kalia (2015); Diversity of Actinomycetes from fodder leguminous plants and their biocontrol potential Int. J. of Adv. Res. 3 (Aug). 1141-1151] (ISSN 2320-5407). www.journalijar.com
