31Aug 2015

EVALUATION OF PHYTOCHEMICAL, ANTIBACTERIAL, ANTIOXIDANT, AND CYTOTOXIC PROPERTIES OF COCCINIA CORDIFOLIA LEAVES

  • Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi-6205, Bangladesh.
  • Dept. of Biophysical Chemistry, Kyoto Pharmaceutical University, 5 Nakauchi-cho, Misasagi, Yamashina-Ku, Kyoto 607-8414, Japan.
  • Department of Cardiovascular Medicine, Tohoku University Graduate School of Medicine, Tohoku University, Sendai, Japan.
  • Department of systems Neurophysiology, Graduate school of medical and dental science, Tokyo Medical and Dental University, Tokyo, Japan.
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There are numerous medicinal plants that have the potential to treat many diseases, one of them is Coccinia cordifolia (Cc) popularly known as Telakucha in Bangladesh. Biochemical screening revealed the presence of carbohydrates, proteins, lipids, vitamins, minerals, phenol and flavonoid in moderate concentration. The antibacterial activity of the methanol and ethanol extracts of Cc were evaluated against Gram positive bacteria (Staphylococcus aureus, Bacillus cereus, Bacillus subtilis, Staphylococcus pyogenes), Gram-negative bacteria (Escherichia coli, Agrobacterium species, Shigella dysenteriae, Shigella sonnei, Salmonella typhi) using disc diffusion assay. Antioxidant potential of the ethanol extract of the plant leaves was evaluated by using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assay, reducing power and total antioxidant capacity. The extract showed significant activity in all antioxidant assays compared to the reference antioxidant ascorbic acid in a dose dependent manner. Cytotoxicity was studied by brine shrimp lethality bioassay. Water extract showed less antibacterial activity as compared to other extractants like ethanol and methanol. Methanol extract showed highest antibacterial activity compared to the ethanol extract. The DPPH free radical scavenging effect of the ethanol extract was compared with standard antioxidant ascorbic acid. IC50 values were found 3000 µg/ml for the extract and 98±8.9 µg/ml for ascorbic acid. Total antioxidant activity was also found to increase in a dose dependent manner and also showed strong reducing power. Methanol extract showed more toxic effect than the ethanol extract. These results suggest that C. cordifolia may act as a chemopreventative agent, providing antibacterial, antioxidant properties and offering effective protection from free radicals.

[M Sohanur Rahman, M Asaduzzaman, Sirajam Munira, M Muedur Rahman, Mahadi Hasan, Abdul Hai siddique, Shahangir Biswas, Mahabuba Khatun, Minarul Islam, Masudul Hasan Khan, Matiar Rahman, Mohammad Amirul Islam (2015); EVALUATION OF PHYTOCHEMICAL, ANTIBACTERIAL, ANTIOXIDANT, AND CYTOTOXIC PROPERTIES OF COCCINIA CORDIFOLIA LEAVES Int. J. of Adv. Res. 3 (Aug). 384-394] (ISSN 2320-5407). www.journalijar.com

M Sohanur Rahman

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