ISOLATION AND ESTIMATION OF COLCHICINE- A VALUABLE PHYTOCHEMICAL IN THE PLANT PARTS OF IPHIGENIA STELLATA BLATT. USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

An attempt has been made to isolate and estimate the quantity of colchicine in the plant parts of Iphigenia stellata blatt. by using solvent extraction followed by high performance liquid chromatography. Colchicine is a drug of choice for management of gout. It is also used in the treatment of familial mediterranean fever. Colchicine has recently been approved by the US FDA for reducing the risk of myocardial infarction (MI), stroke, coronary revascularization, and cardiovascular death in adult patients with established atherosclerotic disease or with multiple risk factors for cardiovascular disease.Colchicine also induces polyploidy in plants. Pure Colchicine in powder form was isolated from the plant parts namely seeds, corms and capsules of Iphigenia stellatablatt. Our research project study was mainly conducted to estimate the quantity of colchicine present in the plant parts of Iphigenia stellata blatt. and to isolate the same in powder form. In our findings colchicine quantity was 10.6 mg/g in the seeds,1.3 mg/gin the corms and 1.0 mg /1gm in the capsule walls .We have isolated dry pure Colchicine powder from the Iphigenia stellata blatt. plant parts using solvent extraction followed by concentration, purification, crystallisation and drying in vacuum oven. The yield obtained is 410 mg/50 gfrom seeds, 25 mg/50 g from corms and  22 mg /50 g from the capsule walls. We have reported highest amount of colchicine in the Iphigenia stellata seeds.