Ameliorative Effect of Bradykinin Potentiating Factor on Haematological and Biochemical Changes Induced by Indomethacin in model ulcer animals.

Many biologists tried different means of treatment the deleterious effects of indomethacin either by natural products or biological substances. Accordingly, the present study aims to investigate the protective effect of BPF7 as a natural products separated from the venom of jellyfish, Cassiopia andromeda on indomethacin induced some haematological and biochemical changes in model ulcer animals. The results showed that, the oral administration of indomethacin (10mg/kg b.w) daily induced leukopenia, lymphopenia and monocytopenia, but the animals treated orally with indomethacin (10mg/kg b.w) day after the other induced leukocytosis, lymphocytosis and monocytosis. In addition, the indomethacin daily or day after the other induced a significant decrease in RBCs count, haematocrit value (HCT) and haemoglobin (Hb) compared to control group. On the contrary, the animals and the ulceration groups treated with BPF7 did not induce any significant changes in all previous haematological parameters except lymphocytes and monocytes. Also, the results indicated that antioxidant enzymes (GST and CAT) and nitric oxide (NO) contents in liver, kidney and stomach homogenates were inhibited by indomethacin and the BPF7 improved these enzymes and nitric oxide to near control group level. The abnormalities induced by indomethacin in these haematological and biochemical parameters may be related to impaired the bone marrow, liver, kidney and stomach or may be to gastrointestinal bleeding, inhibits erythropoiesis, reduction in the cellular antioxidant enzymes and endothelial NO synthesis in tested tissues. On the other hand, these results indicated that the BPF7 significantly ameliorated the toxic effects exerted by indomethacin. This improvement may be attributed to potentiate the endogenous bradykinin by this factor, stimulate the release of prolactin, proliferation of the bone marrow, lymphopoietic cells and enhanced lymphoid organs.