ASTAXANTHIN SYNTHESIS IN SYNECHOCOCCUS PC7002: OPTIMIZATION AND PRODUCTSECRETIONTO CULTURE MEDIUM
- Applied Plant Sciences, Department of Biology, Technical University of Darmstadt, Schnittspahnstraße 1064287 Darmstadt.
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Synechococcus sp. PCC7002 can produce theastaxanthin precursor β-carotene but not astaxanthin.Using a synthetic ketolase-and a synthetic hydroxylase-gene we modifiedSynechococcuscarotene metabolism to produce astaxanthin. The total cellular content of astaxanthin was further increased by over-expressing the intrinsic phytoene synthase. Besides a beneficial step in astaxanthin production our results indicate for some modifications in the suggestedprocess of carotenoid metabolism. In addition, we were expressing an E. coli ABC-transporter to export astaxanthinand decrease intracellular product accumulation. By this, the synthesis was increased further and significant amounts of astaxanthin can be recovered from culture medium without cell destruction.
[Simon Greulich and Ralf Kaldenhoff (2024); ASTAXANTHIN SYNTHESIS IN SYNECHOCOCCUS PC7002: OPTIMIZATION AND PRODUCTSECRETIONTO CULTURE MEDIUM Int. J. of Adv. Res. (Mar). 255-263] (ISSN 2320-5407). www.journalijar.com
TU Darmstadt, Department of Biology, Applied Plant Sciences
Germany