THE ETHYL ACETATE FRACTION OF PSOROSPERMUM FEBRIFUGUM SPACHROOT BARKS AQUEOUS EXTRACT IS A GOOD STIMULATOR OF HEMATOPOIESIS
Maximin Senou
, Pascal Atchade Tchogou
, Felicienne Agbogba
, Ezeckiel Jacques Lokonon
, Esperance Medoatinsa
, Rose Estelle Kanfon
, Victorien Tamegnon Dougnon
, Pascal Cokou Dossa Agbangnan
, Cyrille Boko
, Frederic Loko
, Anatole Laleye
, Amegnona Agbonon
, Lamine Saïd Baba-Moussa
and Alphonse Sezan
- Experimental and Clinic Biology Laboratory, National School of Applied Biosciences and Biotechnology, National University of Science, Technology, Engineering and Mathematics (UNSTIM), Dassa-Zoume, R. Benin.
- Research Laboratory in Applied Biology, Polytechnic School ofAbomey-Calavi, University of Abomey-Calavi, Cotonou, R. Benin.
- Laboratory of Biomembrane and CellSignaling, Faculty of Sciences and Techniques, University ofAbomey-Calavi, R Benin.
- Laboratory of Study and Research in Applied Chemistry, Polytechnic School ofAbomey-Calavi, University ofAbomey-Calavi, R. Benin.
- Human Biology Unit, Faculty of Health Science, Cotonou, R. Benin.
- Laboratoire de Physiologie/Pharmacologie, Faculte des Sciences, Universite de Lome, R. Togo.
- Laboratoire de Biologie et de TypageMoleculaire en Microbiologie, University of Abomey-Calavi R. Benin.
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PsorospermumfebrifugumSpach. (Clusiaceae) is a medicinal plant found in Benin whose root bark was effective in treating anemia. To identify the family of chemical compounds of this organ was responsible for its hematopoietic efficiency, this work aimed to test the ethyl acetate fraction of the aqueous extract of the plant organ on anemic rats.
Methods: Wistar rats were anemic by phenylhydrazinechloridrate on D0. From day 2 to day 15, some were gavage fed with the ethyl acetate fraction of PsorospermumfebrifugumSpach root bark aqueous extract at 40 or 60 mg / kg / day. Others received either vitafer as a reference drug or distilled water (untreated anemic group). Blood samples were collected from these rats and non-anemic control rats at days 0, 2, 7, 10 and 15 for the blood count and osmotic resistance of red blood cells. Results: At D2, phenylhydrazine significantly decreased hemoglobin and red blood cell number, which were corrected on D7 by the extract fraction with a dose-dependent effect. The extract fraction rapidly stimulated release of macrocytes, immature red blood cells in the first week to compensate the anemia. The extract did not affect blood platelet number, suggesting some specificity of action on the red blood cell line.
Conclusion: The ethyl acetate fraction of Psorospermumfebrifugum root bark aqueous extract stimulated erythropoiesis faster than the crude extract. Its action seemed specific and dose-dependent. It would probably be related to the flavonoids which action mechanism needs to be explored.
[Maximin Senou, Pascal Atchade Tchogou, Felicienne Agbogba, Ezeckiel Jacques Lokonon, Esperance Medoatinsa, Rose Estelle Kanfon, Victorien Tamegnon Dougnon, Pascal Cokou Dossa Agbangnan, Cyrille Boko, Frederic Loko, Anatole Laleye, Amegnona Agbonon, Lamine Saïd Baba-Moussa and Alphonse Sezan (2020); THE ETHYL ACETATE FRACTION OF PSOROSPERMUM FEBRIFUGUM SPACHROOT BARKS AQUEOUS EXTRACT IS A GOOD STIMULATOR OF HEMATOPOIESIS Int. J. of Adv. Res. 8 (Oct). 1018-1026] (ISSN 2320-5407). www.journalijar.com
Experimental and Clinic Biology Laboratory, National School of Applied Biosciences and Biotechnology, National University of Science, Technology, Engineering and Mathematics (UNSTIM), Dassa-Zoumé, R. Benin. 2 Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Cotonou, R. Benin.
