15Oct 2019

INCIDENCE OF INDUCIBLE CLINDAMYCIN RESISTANCE IN CLINICAL ISOLATES OF STAPHYLOCOCCI AND THEIR ANTIBIOTIC SENSITIVITY PATTERN

  • Associate Professor, Department of Microbiology, MGM Medical College, Aurganabad, Maharashtra.
  • M.Sc Medical Microbiology student, Department of Microbiology, MGM Medical College, Aurganabad, Maharashtra.
  • Professor & Head, Department of Microbiology, MGM Medical College, Aurganabad, Maharashtra.
  • Assistant Lecturer,Department of Microbiology, MGM Medical College, Aurganabad, Maharashtra
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Introduction: Methicillin resistant Staphylococcus aureus (MRSA) & Methicillin resistant Coagulase Negative Staphylococci(MRCoNS) are recognized as one of the most common organisms causing nosocomial and community-acquired infections and are multiple drug resistant. Clindamycin is one of the preferred agents to treat infections because of these bacteria. But they may develop resistance to clindamycin during therapy called as inducible clindamycin resistance leading to treatment failure. The present study aims at knowing the incidence of inducible clindamycin resistance in clinical isolates of staphylococci and their antibiogram.Materials and Methods: The detection of inducible clindamycin resistance was performed by D-test using erythromycin and clindamycin discs as per CLSI guidelines. Standard disc diffusion test was performed to know the antibiogram for staphylococci.Results: The incidence of MRSA in the present study was 21.46 % and for MRCoNS was 39.94 % .The incidence of inducible clindamycin resistance for MRSA and MRCoNS was 65% and 17.30% respectively. For staphylococci the best sensitivity result was for linezolid.Conclusion: In the present study the incidence of methicillin resistance in Staphylococci was high. Therefore performing the D test is must for identifying true clindamycin resistance to bring out beneficial treatment out comes in the patients.

  1. Mehdinejad M, Sheikh AF, Jolodar A. Study of methicillin resistance in staphylococcus aureus and species of coagulase negative staphylococci isolated from various clinical specimens. Pak J Med Sci 2008; 24(5):719-24.
  2. Povazan A, Vukelic A, Kuruchin T, Handnadev M, Milosevic V, Gusman V. Non-susceptibility trend among Methicillin-Resistant Coagulase-Negative Staphylococci Isolated from Blood Cultures, Institute for Pulmonary Diseases of Vojvodina, 21204 Sremska ?Kamemica, Serbia Medical Faculty, University of Navi Sad, Serbia.2014? Bio. Sci. Belgrade. 66(1), 79-86.
  3. Kircher S, Dick N, Ritter V, Sturm K, Warns P. Detection of Methicillin-Resistant Staphylococcus aureus using a new medium, BBLTM CHROM agar TM MRSA, Compared to Current Methods. As presented at the 104th General Meeting of the American Society for Microbiology, New Orleans, LA,2004.BD diagnostics-7 Loveton Circle ?Sparks, MD USA 21152.
  4. Delialioglu N, Asian G, Ozturk C, Baki V, Sen S, Emekdas G. inducible Clindamycin resistance in Staphylococci isolates from clinical samples. JpnJ Infect Dis 2005; 58:104-6.
  5. Sumit Kumar et al Inducible Clindamycin resistance in staphylococcus isolates from a tertiary care hospital in Eastern India. Ann Trop Med Public Health 2012; 5:468-70.
  6. Sreenivasulu Reddy, R. Suresh. Phenotypic detection if inducible Clindamycin resistance among the clinical isolates of staphylococcus aureus by using the lower limit of inter-disk space. J. microbial Biotech. Res., 2012, 2(2):258-264.
  7. Steward CD, Raney PM, Morrell A k et al. Testing for inducible Clindamycin resistance in erythromycin-resistance of Staphylococcus aureus, J Clin Microbiol 2005; 43:1716-21.
  8. Deotale V. Mendiratta DK Raut U, Narang P. Inducible Clindamycin resistance in Staphylococcus aureus from clinical samples. India J Med Microbiol. 2010; 28:124-6.
  9. Clinical and laboratory standards institute. Performance standards for antimicrobial susceptibility informational supplements. Clinical laboratory Standard Institute. 2007; Vol. 2(No.1)(if use)
  10. Monika Cheesbrough. District Laboratory Practice in Topical Countries. 2006; 2nd, Part-II: P. 38-39.
  11. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fourth Informational Supplement. CLSI document M100-S24. Wayne, PA: Clinical and Laboratory Standards Institute. 2014.
  12. Leandro R., Juliana C. et al Use of the D Test Method to Direct Inducible Clindamycin Resistance in Coagulase Negative Staphylococci (CoNS) The Brazilian Journal of Infectious Diseases 2007;11(2):186-188.
  13. Yilmaz G, Aydin K, Iskender S, Caylan R, Koksal I. Detection and prevalence of inducible clindamycin resistance in staphylococci. J Med Microbiol 2007; 56: 342-5.
  14. Fokas S, Fokas S, Tsironi M, Kalkani M, Diony Sopouloy M. Prevalence of inducible clindamycin resistance in macrolide-resistant Staphylococcus Clin Microbiol Infect 2005; 11 : 337-40.
  15. Lall M, Sahni AK. Prevalence of inducible clindamycin resistance in Staphylococcus aureus isolated from clinical samples. Med J Armed Forces India 2014; 70: 43-7.
  16. Susana C, Bu?lent B, Kathy Katz, Inducible Clindamycin Resistance and Molecular Epidermilogic Ternds of Pediatric Community-Acquired Methicillin-Resistance Staphylococcus aureus antimicrobial agents and chemotherapy, June 2005, p. 2283?2288 Vol. 49, No. 6.
  17. Dror S., Zmira S., Itamar S. et al Inducible Clindamycin Resistance among Methicillin-Sensitive Staphylococcus aureus Infection in Pediatric Patients: IMAJ VOL 13 ? October 2011.
  18. Prabhu K, Rao S, Rao V. Inducible Clindamycin Resistance in Staphylococcus aureus Isolated from Clinical Samples. J Lab Physicians.2011; 3(1):25?7.
  19. Abhishek Mehta, Vijay Prakash Singh. Inducible Clindamycin resistance among clinical isolates of Staphylococcus aureus at a rural tertiary care teaching hospital of western Uttarpradesh. Int J Intg Med Sci 2017; 4(3): 472-75. ISSN 2394 ? 4137.
  20. Ratna Baral and Basudha Khanal. Inducible resistance in Staphylococcus aureus strains Isolated from Clinical Samples. International Journal of Biomedical Research 2017; 8(02): 81-84.
  21. Eman Mohamed Zaher. Inducible Clindamycin Resistance in Clinical Isolates of Staphylococci. International Annals of Medicine. 2017; 1(4).
  22. Khadri H and Alzohairy M. Prevalence and antibiotic susceptibility pattern of methicillin-resistant and coagulase-negative staphylococci in a tertiary care hospital in India. International Journal of Medicine and Medical Sciences. April 2010 Vol. 2(4), pp. 116-120.
  23. Angel MR, Balaji V, Prakash JA, Brahmandathan KN, Mathews MS. Prevalence of inducible clindamycin resistance in gram positive organisms in a tertiary care centre. Indian J Med Microbiol 2008; 26: 262‑
  24. Fasih N, Irfan S, Zafar A, Khan E, Hasan R. Inducible clindamycin resistance due to expression of erm genes in Staphylococcus aureus: Report from a Tertiary Care Hospital Karachi, Pakistan. J Pak Med Assoc 2010; 60(9):750-754.
  25. E. Mshana , E. Kamugisha et al Prevalence of Clindamycin inducible resistance among Methicillin-resistance Staphylococcus aureus Tanzania Journal of Health Research, Vol. 11, No. 2, April 2009.
  26. Lyall KS, Gupta V, Chhina D. Inducible clindamycin resistance among clinical isolates of Staphylococcus aureus. J Mahatma Gandhi Inst Med Sci 2013; 18: 112-5.
  27. Jadhav SV, Gandham NR, Sharma MKM. Prevalence of inducible clindamycin resistance among community-and hospital-associated Staphylococcus aureus isolates in a tertiary hospital in India. Biomed Res. 2011; 22(4):465?9.
  28. Date K,Choudhary M, ThombareV. Inducible Clindamycin Resistance in clinical isolates of staphylococci in a rural hospital. Int J Biol Med Res. 2012; 3: 1922-1925.
  29. Shetty J and Afroz Z. Prevalence of constitutive and inducible clindamycin resistance among clinical isolates of Staphylococcus aureus in a tertiary care institute in North India. Int J Res Med Sci. 2017 Jul; 5(7):3120-3125.
  30. Bansal N et al. Prevalence of and inducible clindamycin resistance in clinical isolators of Coagulase negative Staphylococci at a tertiary care hospital. 2012: 5:427-30.
  31. Hatkar S. et al Antimicrobial Profile of Inducible Clindamycin Resistant Strains of Staphylococcus aureus Isolated from Clinical Samples. International Journal of Health Sciences & Research: June 2014: Vol.4; Issue: 6.
  32. Pai V et al. Prevalence and Antimicrobial Susceptibility Pattern of Methicillin-resistant Staphylococcus Aureus [MRSA] Isolates at a Tertiary Care Hospital from Mangalore, South India. Journal of Laboratory Physicians / Jul-Dec 2010 / Vol-2 / Issue-2.

[Bansal V.P, Gadekar K.S, Mulay M.V and Mishra J.K (2019); INCIDENCE OF INDUCIBLE CLINDAMYCIN RESISTANCE IN CLINICAL ISOLATES OF STAPHYLOCOCCI AND THEIR ANTIBIOTIC SENSITIVITY PATTERN Int. J. of Adv. Res. 7 (Oct). 1066-1073] (ISSN 2320-5407). www.journalijar.com

Dr. Bansal V.P
Associate Professor

DOI:

Article DOI: 10.21474/IJAR01/9924      
DOI URL: https://dx.doi.org/10.21474/IJAR01/9924

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