03Jul 2019

PHYTOCHEMICAL SCREENING, PROXIMATE COMPOSITION AND HEAVY METALS OF CAMELLIA SINENSIS LEAVES.

  • Fibre & Polymer Research Division, BCSIR Laboratories Dhaka, Bangladesh Council of Scientific and Industrial Research, Dhanmondi, Dhaka-1205, Bangladesh.
  • Institute of National Analytical Research and Service (INARS), Bangladesh Council of Scientific and Industrial Research, Dhanmondi, Dhaka-1205, Bangladesh.
Crossref Cited-by Linking logo

  • Abstract
  • Keywords
  • References
  • Cite This Article as
  • Corresponding Author

Phytochemical screening, proximate compositions and some heavy metals were assessed on the leaves of Camellia sinensis Linn. Phytochemical screening indicated presence of alkaloids, flavonoids, carbohydrates, tannins, terpenoids, reducing sugars, cardiac glycosides, steroids and saponins. Proximate compositions analysis showed that the percentage of moisture, ash, crude protein, crude fat and crude fibre contents were 9.46% ? 0.31, 7.30% ? 0.40, 13.65% ? 0.69, 2.72%?0.12 and 7.69% ? 0.26 respectively. Spectrophotometric analysis showed that the percentage of heavy metals like Pb, As, Cd and Hg content were found 0.56 ? 0.05,

  1. Adegboye, M. F., Akinpelu, D. A. and Okoh, A. (2008): The bioactive and phytochemical properties of Garcinia kola (Heckel) seed extract on some pathogens. African Journal of Biotechnology, 7(21):3934-3938.
  2. Adinortey, M. B., Sarfo, J. K., Quayson, E. T., Weremfo, A., Adinortey, C. A., Ekloh, W. and Ocran, J. (2012): Phytochemical Screening, Proximate and Mineral Composition of Launaeataraxacifolia Research Journal of Medicinal Plant, 6(2): 171-179.
  3. Agency for Toxic Substances and Disease Registry (ATSDR)(1999): Toxicological Profile for Cadmium and Nickel, Agency for Toxic Substances and Disease Registry, US Department of Health and Human Services, Public Health Service, 205-93-0606.
  4. Agency for Toxic Substances and Disease Registry (ATSDR)(1999): Toxicological Profile for Lead, Agency for Toxic Substances and Disease Registry, US Department of Health and Human Services, Public Health Service, 205-93-0606.
  5. AOAC, (1990): Official Method of Analysis 15th , Association of Analytical Chemist. Washington DC, USA.
  6. Bailey, R. (2008): The Role of Proteins in the Body, About.com Guide to Biology.
  7. Bajai, A. Meyers (2001): ?Effect of Natural extract of pineapple on disstibility, performance traits and nitrogen balance of broiler chicks??. Australian Journal of Basic and applied Sciences, 5(20):10-30.
  8. Bhattacharya, S., Das, S. K. and Haldar PK (2014): Arsenic induced myocardial toxicity in rats: Alleviative effect of Trichosanthesdioica J. Diet. Suppl.,11: 248-261.
  9. Chin, Y. W., Balunas, M. J., Chai, H. B. and Kinghorn, A. D. (2006): Drug discovery from natural sources. AAPS J.,8: E239-53.
  10. De Bruyne, T., Pieters, L., Deelstra, H. and Vlietinck, A. (1999): Condensed vegetables tannins: biodiversity in structure and biological activities.? System. Ecology, 27: 445?459.
  11. Dhale, D. A., and Mogle, U. P. (2011): Phytochemical and Antibacterial Activity of Phyllanthusemblica (L.). Science Research Reporter, 1: 138-142.
  12. Dhawan, V. and Jain, S. (2005): Garlic supplementation prevents oxidative DNA damage in essential hypertension. Molecular and Cellular Biochemistry, 275: 85-94.
  13. Dolara, P., Luceri, C., De Filippo, C., Femia, A. P., Giovannelli, L., Carderni, G., Cecchini, C., Silvi , S., Orpianesi, C. and Cresci, A. (2005): Red wine polyphenols influence carcinogenesis, intestinal microflora, oxidative damage and gene expression profiles of colonic mucosa in F344 rats. Mutation Res., 591: 237?246.
  14. Dougnon, T.V., Bankole, H.S., Johnson, R.C., Klotoe, J.R.,Dougnon, G., Gbaguidi, F., Assogba, F., Gbenou, J., Sahidou, S., Ategbo, J.M., Rihn, B.H., Loko, F., Boko, M. and Edorh, P.A. (2012): Phytochemical screening, nutritional and toxicological analyses of leaves and fruits of Solanummacrocarpon(Solanaceae) inCotonou(Benin).Food Nutr. Sci., 3(11): 1595-1603.
  15. Dwivedi, S. K. andDey, S. (2002): Medicinal herbs: A potential source of toxic metal exposure for man and animals in India. Arch. Environ. Health, 57(3):229-231.
  16. Flora, S.J.S. (2009): Structural, chemical and biological aspects of antioxidants for strategies against metal and metalloid exposure. Oxid.Med.Cell. Longevity, 2(4): 191?206.
  17. Ganong, W. F. (2003): Circulating Body Fluids: Review of Medical Physiology. 21st , Typo Press, Lebanon.
  18. Gupta, D. A., Bhaskar, D. J., Gupta, R. K., Karim, B., Jain, A. and Dalai, D. R. (2014): Green tea: a review on its natural anti- oxidant therapy and cariostatic benefits. Biol.Sci.Pharma. Res., 2(1):008-012.
  19. Gupta, S.S. (1994): Prospects and perspective of natural plant products. Indian J. Pharmacol., 26(1): 1-12.
  20. Harborne, J. B. (1973): Phytochemical methods, London. (Chapman and Hall, Ltd), pp 49-188.
  21. Houghton, P. J., Woldemariam, T. Z., O?Shea, S., Mullen, J. E., Rostron, J., Bantvala, J. E., Walker, E., Thyagarajan, S. P., Soloman, S. and Yeap, F. L. (1999): The anti-HIV activity of an aqueous extract and polyphenoliccompounds ? J.? Pharm. Pharmacol., 51: 100.
  22. Igile, G.O., Iwara, I.A., Mgbaje, B.A., Uboh, F.E. and Ebong, P.E. (2013): Phytochemical, proximate and nutrient composition of VernoniacalvaonaHook (Astereceae): A green ?leafy vegetable in Nigeria. J. food Res., 2(6): 1?11
  23. James, C. S., (1995): Analytical Chemistry of Foods, 1st, Chapman and Hall, New York.
  24. Jeeshna, M. V., Paulsamy, S. and Mallikadevi, T. (2011): Phytochemical Constituents and Antimicrobial Studies of the Exotic Plant Species, Croton bonplandianum J. Life Sci., 3: 23-27.
  25. Kabir, O. A., Olukayode, O., Chidi, E. O., Christopher, C. I., and Fasure, K. A. (2005): Screening of crude extracts of six medicinal plants used in South west Nigerian unorthodox medicine for anti-methicillin resistant Staphylococcus aureus BMC Complementary and Alternative Medicine, 5: 6.
  26. Kirk, R. S. (1993): Analysis of Food.In: Encyclopedia of Food Science, Food Technology and Nutrition:Macrae, R., Robinson, R. K., Sadler, M. J. ed. (Academic Press, New York), pp 183 - 188.
  27. Klaassen, C. D. (2001): Casarett and Doull's toxicology: the basic science of poisons. (New York, USA: McGraw-Hill).
  28. Krishna, D. G., Alkindi, J., Alshukili, M., Alhabsi, A. and Devi, C. K. (2014): Int. J. of Inorganic and Bioinorganic Chemistry, vol. 5, no.1, 16-18.
  29. Li, H., Wang, Z. and Liu, Y. (2003): Review in the studies on tannins activity of cancer prevention and anticancer. Zhong-Yao-Cai, 26(6):444-448.
  30. Moeller, S. M., Jacques, P. F. and Blumberg, J. (2000): The potentia role of dietary xanthophylls in cataract and age-related macular degeneration. Journal of the American College of Nutrition, 19: 522- 527.
  31. Nordberg, G. (1999): Excursions of intake above ADI: case study on cadmium. Regul.Toxicol.Pharmacol., 30: S57-S62.
  32. Okwu D. E. and Emenike I. N. (2006): Evaluation of the phytonutrients and vitamin contents of Citrus fruits. Int. J. Mol. Med. Adv. Sci. 2:1?6.
  33. Omoregie, E. S., Oriakhi, K., Oikeh, E. I.,Okugbo, O. T. and Akpobire, D. (2014): Comparative study of phenolic content and antioxidant activity of leaf extracts of Alstoniaboonei and Eupatorium odoratum. Nig. J. Basic Appl. Sci., 22(3&4): 91-97.
  34. Oseni, O. A. and Okoye, V. I. (2013): Studies of phytochemical and antioxidant properties of the fruit of watermelon (Citrulluslanatus) (Thunb). ? Pharm. Biomedical Sci., 27(27): 508-514.
  35. Pcerangeli, G., Vital, G. and Rivera, W. (2009): Antimicrobial activity and cytotoxicity of Chromolaenaodorata (L. F.) king and rabinson and Uncariaperrottetii (A. Rich) Merr extracts. J. Med. Plants Res.,3(7):511-8.
  36. Pearson, D.(1976): The Chemical Analysis of Foods. 7th , Churchill Livingstone, London, ISBN: 0443014116, pp:7-11.
  37. Ratnaike, R. N. (2003): Acute and chronic arsenic toxicity. Postgrad. Med. J.,79: 391-396.
  38. Risher, J. F., WHO (2003): Elemental mercury and inorganic mercury compounds: human health aspects, 50, World Health Organization,Geneva, Switzerland.
  39. Sermakkani, M. andThangapandian, V. (2012): GC-MS analysis of Cassia itallica leaf methanol extract. Asian J. Pharm.Clin. Res., 5(2):90-4.
  40. Sharma, M., Nagori, K., Soni, S., Swarnakar, H., Vaishnav, S., Nazir, Md., Khan, N. andPandy, T. (2014): International Journal of Pharmaceutical and Chemical Sciences, 3(1): 110-117.
  41. Singh, K. P., Bhattacharya, S. and Sharma, P. (2014): Assessment of heavy metal contents of some Indian medicinal plants. American- Eurasian J. Agric. Environ. Sci.,14: 1125-1129.
  42. Sofowara, A. (1993): Medicinal Plants and Traditional Medicine in Africa.2nded, (Spectrum Books Ltd, Ibadan, Nigeria). pp191-289.
  43. Tangka, J.K. (2003). Analysis of the thermal energy requirements for the extraction of leaf protein concentrate from some green plants. Biosystems Eng., 86(4): 473-479.
  44. Todd,G. C. (1996): Vegetables Grown in Mine Wastes. Environ. Toxicol.and Chem., 19(3): 600-607.
  45. Tong, S., von Schirnding, Y. E. andPrapamontol, T. (2000): Environmental lead exposure: a public problem of global dimension. Bull World Health Organ.,78: 1068-1077.
  46. Trease, G. E. and Evans, W. C. (1989):Pharmacognsy.11th(BrailliarTiridel Can. Macmillianpublishers).pp45-50.
  47. UgwuOkechukwu, P. C, NwodoOkwesili, F. C, Joshua Parker, E., BawaAbubakar, Ossai Emmanuel, C. and Odo Christian, E. (2013): Phytochemical and Acute Toxicity Studies ofMoringaoleifera Ethanol Leaf Extract. International Journal of Life Sciences Biotechnology and Pharma Research, 2(2):66-71.
  48. WHO (2007): Guidelines for assessing quality of herbal medicines with reference to contaminants and residues. World Health Organization, Geneva, Switzerland.
  49. Yadav, R. N. S. and MuninAgarwala.(2011): Phytochemical analysis of some medicinal plants. Journal of Phytology, 3(12):10-14.
  50. Yukari, I., Youichi, F., Ikuko, N. andItsuru, Y. (1995): Quantitative HPLC analysis of cardiac glycosides in Digitalis purpurea leaves. J. Nat. Prod., 58(60):897-901.

[Mohammad Majedul Haque, Md. Rashed Hasan, Shakila Akter, Md. Khabir Uddin Sarker, Shamima Akther Eti and Shahin Sultana. (2019); PHYTOCHEMICAL SCREENING, PROXIMATE COMPOSITION AND HEAVY METALS OF CAMELLIA SINENSIS LEAVES. Int. J. of Adv. Res. 7 (Jul). 195-201] (ISSN 2320-5407). www.journalijar.com

Shahin Sultana
Senior Scientific Officer, Bangladesh Council of Scientific and Industrial Research, Dhaka-1205, Bangladesh

DOI:

Article DOI: 10.21474/IJAR01/9345      
DOI URL: https://dx.doi.org/10.21474/IJAR01/9345

Download Full Paper

Download PDF No. of Downloads: 29 | No. of Views: 97