20Jun 2019

MELISSOPALYNOLOGY AND CLIMATIC VARIATION: CASE OF HONEY ATTRIBUTED TO EUPHORBIA RESINIFERA, REGION OF AZILAL (MOROCCO).

  • Laboratory of Applied Geology and Geo-environment, Faculty of Sciences, IbnZohr University, Agadir Morocco.
  • Laboratory of Biotechnologies and Valorization of Natural Resources, Faculty of Sciences, IbnZohr University, Agadir Morocco.
  • Abstract
  • Keywords
  • References
  • Cite This Article as
  • Corresponding Author

This work is an example of the use of honey in the study of the impact of global warming on nectar production and the relationship between plants and pollinators. It is the result of the melissopalynological analysis of 42 honey samples attributed to Euphorbia resinifera Berg, from the region of Azilal (Morocco). Quantitative analysis showed that these honeys are mostly poor in botanical elements. Although beehives were established during the flowering period of Euphorbia resinifera, in areas dominated by it, only 16.7% of honeys harvested showed the monofloral character associated with the species. The others are multifloral or honeydew produced mainly by the holm oak (Quercus ilex). The qualitative analysis of the honeys showed the presence of 55 botanical taxa belonging to 24 families. Pollen from herbaceous species is better represented than pollen from shrubs and trees. Entomophiles are dominant. The evolution of the pollen percentages of E. resinifera in the pollen spectrum has shown that the precocity of its flowering related to the increase of the temperature and the diminution of the precipitations has negatively influenced its nectar production, its attraction for bees, and its contribution to the elaboration of honey.


  1. Aafi, A., Taleb, M. S., Fechtal M. (2002)?: Esp?ces remarquables de la flore du Maroc. Centre National de la Recherche Foresti?re BP. 763 Agdal-Rabat Maroc, p. 52.
  2. Aazza, S., Lyoussi, B., Antunes, D., Miguel M.G. (2014): Physicochemical characterization and antioxidant activity of 17 commercial Moroccan honeys. Int J Food SciNutr., 65: 449-457.
  3. Adgaba, N., Al-Ghamdi, A., Tadesse, Y., Getachew, A., Awad, A. M., Ansari, M. J., Owayss, A. A., Mohammed, S. E. A., Alqarni, A. S. (2017): Nectar secretion dynamics and honey production potentials of some major honey plants in Saudi Arabia. Saudi J Biol Sci., 24: 180?191.
  4. Adgaba, N., Awad, M.A., Al-Ghamdi, A.A., Alqarni, A.S., Radloff, S.E. (2012): Nectar of Ziziphusspina-christi (L.) Willd (Rhamnaceae): dynamics of secretion and potential for honey production. Apic. Sci., 56: 49?59.
  5. Ait Brahim, Y., Bouchaou, L. ,Sifeddine, A. , Khodri, M. , Reichert, B. , Cruz, F. W. (2016): Elucidating the climate and topographic controls on stable isotope composition of meteoric waters in Morocco, using station-based and spatially-interpolated data. ‎ Hydrol., 543: 305-315.? DOI: 10.1016/j.jhydrol.2016.10.001
  6. Alqarni, A.S. (2015): Honeybee foraging, nectar secretion, and honey potential of wild jujube trees, Ziziphusnummularia. Entomol., 44: 232?241.
  7. Anderson, J.T., Inouye, D., Mckinney, A., Colautti, R., Mitchell-Olds, T. (2012): Phenotypic plasticity and adaptive evolution contribute to advancing flowering phenology in response to climate change. R. Soc. B., 279: 3843?3852.
  8. Anonymous (2012)?: Arr?t? du ministre de l?agriculture et de la p?che maritime N?1721-12 Reconnaissance de l?IGP ? Miel d?Euphorbe Tadla-Azilal ? et homologation du cahier des charges y aff?rent?: Bulletin officiel n? 6074, p. 2524-2525.
  9. Anonymous (2014)?:?Royaume du Maroc: Cinqui?me rapport national sur la mise en ?uvre de la convention sur la diversit? biologique, Rabat, p. 135.
  10. Atanassova, J., Lazarova, M., Yurukova, L. (2016): Significant parameters of Bulgarian honeydew honey. Cent. Eur. Agric., 17: 640-651.
  11. Bakke, A. L. (1936): Leafy spurge, Euphorbia esula Iowa Agric. Exp. Stn. Res. Bull., 198: 209?245.
  12. Benabid A. (2000)?: Flore et ?cosyst?me du Maroc : ?valuation et pr?servation de la biodiversit?. In?: Ibis Press, Paris, p. 80.
  13. Bettar, I., Gonzalez-Miret, M.L., Hernanz, D., Marconi, A., Heredia F.J., Terrab A. (2015): Characterisation of Moroccan Spurge (Euphorbia) honeys by their physicochemical characteristics, mineral contents and color. Arab J Chem., doi:10.1016/j.arabjc.2015.01.003.
  14. Bogdanov, S., L?llmann, , Martin, P., von der Ohe, W. , Russmann, H., Vorwohl, G., PersanoOddo, L., Sabatini, A. G., Marcazzan, G. L., Piro, R., Flamini, C., Morlot, M., Lheretier, J., Borneck, R., Marioleas, P., Tsigouri, A., Kerkvliet, J., Ortiz, A., Ivanov, T., D\'Arcy, B., Mossel, B., Vit, P. (1999): Honey quality, methods of analysis andinternational regulatory standards: review of the work of the International Honey Commission. Mitt. Lebensm. Hyg., 90: 108-125.
  15. Bogdanov, S., Ruoff, K., PersanoOddo, L. (2004): Physico-chemical methods for the characterisation of unifloral honeys: a review. , 35: 4?17.
  16. Bolten, A. B., Feinsinger P. (1978): Why do hummingbird flowers secrete dilute nectar?., 10: 307-309.
  17. Boose D.L. (1997): Sources of variation in floral nectar production rate in Epilobiumcanum(Onagraceae): Implications for natural selection. , 110: 493-500.
  18. Bouhlali, E.D.T., Bammou, M., Sellam, K., Ramchoun, M., Benlyas, M., Alem, C., Filali-Zegzouti, Y. (2016): Evaluation of antioxidants, antibacterial and antifungal activities of eleven monofloral honey samples collected from Morocco. Chem. Pharm. Res., 8: 299?306.
  19. Burghardt, L.T., Runcie, D.E., Wilczek, A.M., Cooper, M.D., Roe, J.L., Welch, S.M., Schmitt, J. (2016): Fluctuating, warm temperatures decrease the effect of a key floral repressor on flowering time in Arabidopsis thaliana. New Phytol., 210: 564?576. http://dx.doi.org/10.1111/nph.13799.
  20. Carri?n-Tacuri, J., Berjano, R., Guerrero, G., Figueroa, M.E., Tye, A., Castillo, J.M. (2012): Nectar production by invasive Lantana camaraand endemic peduncularisin the Gal?pagos Islands. Pacific Science., 66: 435-445.
  21. Carroll A.B., Pallardy S.G., Galen C. (2001): Drought stress, plant water status, and floral trait expression in fireweed, Epilobiumangustifolium(Onagraceae). J. Bot., 88: 438?446.
  22. Chakir, A., Romane, A., Marcazzan, G. L., Ferrazzi, P. (2016): Physicochemical properties of some honeys produced from different plants in Morocco. J. Chem., 9: 946-954.
  23. Chambers, L.E.?, Altwegg, R.?, Barbraud, C.?, Barnard, P.?, Beaumont, L.J.?, Crawford, R. J. M.?, Durant, J. M.?, Hughes, L.?, Keatley, M. R.?, Low, M.?, Morellato, P. C.?, Poloczanska, E. S.?, Ruoppolo, V.?, Vanstreels, R. E. T.?, Woehler, E. J.?, Wolfaardt, A. C. (2013): Phenological Changes in the Southern Hemisphere. PLoS ONE., 8: e75514. doi:10.1371/journal.pone.0075514
  24. Chmielewski, F-M., Bl?mel, K., Hennige, Y., Blanke, M., Weber, R.W.S., Zoth M. (2011): Phenological models for the beginning of apple blossom in Germany. Z., 20: 487?496
  25. Corbet, S. (1978): Bee visits and the nectar of Echiumvulgare and Sinapisalba L. Ecol. Entomol., 3: 25-37.
  26. Crousilles, A. (2014)?: Usages, propri?t?s antibact?riennes et physicochimie de miels marocains. Dipl?me d\'?tat de Docteur en Pharmacie, Universit? de Montpellier I, Montpellier.
  27. Damblon F. (1988)?: Caract?risation botanique, ?cologique et g?ographique des miels du Maroc. Travaux de la Section Scientifique et Technique, Institut Fran?ais de Pondichery., 25?: 309?329.
  28. Defila, C. and Clot B. (2001): Phytophenological trends in Switzerland. Int J Biometeorol., 45: 203?207
  29. Fennane, M., Ibn Tattou, M., Ouyahya, A., Mathez, J., El Oulaidi, J. (2007): Flore pratique du Manuel de d?termination des plantes vasculaires, volume 2. Travaux de l?Institut Scientifique, S?r. Botanique, Rabat. p.?126.
  30. Foley, M.E., Anderson, J.V., Horvath. D.P. (2009): The effects of temperature, photoperiod, and vernalization on regrowth and flowering competence in Euphorbia esula(Euphorbiaceae) crown buds. , 87: 986-992.
  31. Franks, S. J.; Sim, S.; Weis, A.E. (2007): Rapid evolution of flowering time by an annual plant in response to a climate fluctuation. Proc. Natl. Acad. Sci. U. S. A, 104: 1278?1284
  32. Galitz, D.S., Davis, D. G. (1983): Leafy Spurge Physiology and Anatomy. Dak. Farm Res., 20: 20-26.
  33. Gallagher, R. V., Hughes, L., Leishman, M. R. (2009): Phenological trends among Australian alpine species: using herbarium records to identify climate-change indicators. J. Bot., 57: 1-9, https://doi.org/10.1071/BT08051
  34. Gattefosse, J. and Vindt, J. (1954): Les Euphorbes cactiformes du Maroc. , 41?: 65-72.
  35. Gesch, R. W., Palmquist, D., Anderson, J. V. (2007): Seasonal Photosynthesis and Partitioning of Nonstructural Carbohydrates in Leafy Spurge (Euphorbia esula). , 55: 346-351
  36. G?miz-Garc?a, F. (2001): Flora selecta marroqu?. Francisco Javier Navarro D?ez: Am?bar, SL., Burgos, p. 140.
  37. Hanson, H.C. and Rudd, V.E. (1933): Leafy spurge life history and habits. North Dakota Agric. ColI. Agric. Exp. Stn. Bull., 226: 1-24.
  38. Hovenden, M. J., Wills, K. E., Schoor, J. K. V., Williams, A. L., and Newton, P. C. D. (2008): Flowering phenology in a species-rich temperate grassland is sensitive to warming but not elevated CO2. New Phytolo., 178: 815?822. doi: 10.1111/j.1469-8137.2008.02419.x
  39. Hunt, E. R., Mcmurtrey, JR., J. E., Williams, A. E. P., Corp, L. A. (2004): Spectral characteristics of leafy spurge (Euphorbia esula) leaves and flower bracts. Weed Sci., 52: 492?497.
  40. Jaafar, K., Haidar, J., Kuraydiyyah, S., Ghaddar, T., Knio, K., Ismail B., Toufeili I. (2017): Physicochemical, melissopalynological and antioxidant properties of artisanal honeys from Lebanon. Food Sci. Technol., 54: 2296-2305.
  41. Lake, J. C. and Hughes, L. (1999): Nectar Production and Floral Characteristics of Tropaeolummajus Grown in Ambient and Elevated Carbon Dioxide. Ann Bot., 84: 535?541.
  42. Louveaux, J., Maurizio, A., Vorwohl, G. (1970)?: Commission Internationale De Botanique Apicole De L?U.I.S.B.: Les m?thodes de la m?lissopalynologie. Apidologie., 1: 211-227.
  43. Louveaux, J., Maurizio, A., Vorwohl, G. (1978): Methods of Melissopalynology, Bee World., 59: 139-157.
  44. Mačukanović-jocić, M. and Djurdjević, L. (2005): Influence of microclimatic conditions on nectar exudation in Glechomahirsuta K. Arch. Biol. Sci., 57: 119-126.
  45. Mačukanović-Jocić, M., DajićStevanović, Z., Jarić, S., Đurđević L. (2008): Nectar secretion in basil (Ocimumbasilicum) grown in different soil conditions. J. Apic. Res., 47: 89-90. DOI: 10.1080/00218839.2008.11101431
  46. Mateo, R. and Bosch-Reig, F. (1998): Classification of Spanish Unifloral Honeys by Discriminant Analysis of Electrical Conductivity, Color, Water Content, Sugars, and pH. J. Agric. Food Chem., 46: 393−400.
  47. Maurizio, A. (1979): Microscopy of honey. In: A comprehensive survey, Crane (ed). Heinemann: London, pp. 240 ? 257.
  48. Menzel, A., Estrella, N., Testka A. (2005): Temperature response rates from long-term phenological records. Res., 30: 21?28.
  49. Menzel, A., Sparks, T.H.?,Estrella, N., Koch, E., Aasa, A.?, Ahas, R.?, Alm-Kubler, K.?, Bissolli, P.?, Braslavska, O.?, Briede ?et al. (2006): European phenological response to climate change matches the warming pattern. Glob Chang Biol., 12: 1969?1976.
  50. Mitchell, R.J. (1993): Adaptive significance of Ipomopsisaggregata nectar production: observation and experiment in the field. , 47: 25-35.
  51. Mitchell, R.J. and Waser, N. M. (1992): Adaptive significance of Ipomopsisaggregatanectar production: Pollination success of single flowers. , 73: 633-638.
  52. Mokssit, A. (2012)?: L??tat du changement climatique et ses impacts au Maroc?: Le point sur le changement climatique au Maroc. In?: Environnement et Changement Climatique au Maroc. Diagnostic et Perspectives (ed). Zeino-Mahmalat E., Bennis A., Eds., Konrad-Adenauer-Stiftung E. V., Rabat, Maroc., p. 35-39.
  53. Moore, P. D., Collinson, M., Webb, J. A. (1991): Pollen Analysis. Blackwell scientific publications. 2ed (ed). Blackwell Scientific Publications: Oxford, p. 216.
  54. Moujanni, A., Terrab, A., Eddoha, R., Nasser, B., Benbachir, M., Chaouqy, N. E.?,Bouzid, T.?, Essamadi, A. K. (2017): Microbiological quality of Moroccan labeled Euphorbia resinifera J. microbiol. biotechnol. food sci., 6: 1188-1194.
  55. Naman, M., Faid, M., El Adlouni, C. (2005): Microbiological and physico-chemical properties of Moroccan honey. Int J Agric Biol., 7: 773?776.
  56. Nedić, N., Mačukanović-Jocić, M., Rančić, D., Rr?slett, B., ?o?tarći, I., Stevanović, Z.D., Mladenović, M. (2013): Melliferous potential of Brassica napus L. subspnapus (Cruciferae). -Plant Int., 7: 323-333. DOI: 10.1007/s11829-013-9247-2
  57. Ohe, W. Von Der, PersanoOddo, L., Piana, M.L., Morlot, M., Martin, P. (2004): Harmonized methods of melissopalynology. , 35: 18?25.
  58. PersanoOddo, L. and Piro R. (2004): Main European unifloral honeys: descriptive sheets, , 35: 38?81.
  59. Petanidou, T., Smets E. (1996): Does temperature stress induce nectar secretion in Mediterranean plants? New Phytologist., 133: 513-518.
  60. Petretto, G.L., Tuberoso, C.I.G., Fenu, M.A., Rourke, J.P., Belhaj, O., Pintore G. (2017): Antioxidant activity, color chromaticity coordinates, and chemical characterization of monofloral honeys from Morocco. INT J FOOD PROP., 20: 2016-2027.
  61. Rather, R.N., Wani, A.A., Kashtwari,M.,? Beigh, Z.A. (2018): Phenological shifts due to climate change and the associated conservation threats. Climate Change., 4(13): 80-86.
  62. Rawal, D. S., Kasel, S., Keatley, M. R., Nitschke, C. R. (2014): Herbarium records identify sensitivity of flowering phenology of eucalypts to climate: Implications for species response to climate change. Austral Ecol., 40?: 117?125. doi:10.1111/aec.12183
  63. Reille, M. (1992)?: Pollen et spores d?Europe et d?Afrique du nord, Laboratoire de botanique historique et palynologie, Marseille, France. 520?p.
  64. Roubik, D.W. and Buchmann, S.L. (1984): Nectar selection by Melipona and Apismellifera (Hymenoptera: Apidae) and the ecology of nectar intake by bee colonies in a tropical forest. , 61: 1?10.
  65. Rumpff, L., Coates, F., Morgan J.W. (2010): Biological indicators of climate change: evidence from long-term flowering records of plants along the Victorian coast, Australia. J. Bot., 58: 428-439. https://doi.org/10.1071/BT10053
  66. Sierra, A., G?mez, P.A., Gonell Galindo J. (1987)?:Composici?n, propiedades f?sico-qu?micas y espectro pol?nico de algunas mieles monoflorales de Espa?a. , 185: 61-84.
  67. John, L. and Tilley D. (2014): Plant Guide for Leafy spurge (Euphorbia esula). USDA-Natural Resources Conservation Service, Plant Materials Center. Aberdeen, Idaho, 83210. 4p.
  68. Terrab, A., Berjano, R., Sanchez, J. A., Pajuelo, A.G., D?ez, M.J. (2019): Palynological and geographical characterisation of Spanish oak honeydew honeys. , 58: 63-77. DOI: 10.1080/00173134.2018.1509124
  69. Terrab, A., Marconi, A., Bettar, I., Msanda, F., D?EZ M.J. (2014): Palynological Characterization of Euphorbia Honeys from Morocco, Palynology., 38: 138-146.
  70. Vald?s, B., Díez, M.J., Fernández, I., Barneto, C. (1987): Atlas pol?nico de Andaluc?a occidental. Instituto de Desarrollo Regional 43, Universidad de Sevilla Excma. Diputaci?n de C?diz., 450 p
  71. Waller, G. D. (1972): Evaluating responses of honey bees to sugar solutions using an artificial flower feeder. entom. Soc. Am., 65: 857-862.
  72. White, F. (1983): The Vegetation of Africa: A Descriptive Memoir to Accompany the UNESCO/AETFAT/UNSO Vegetation Map of Africa. UNESCO, Paris. p. 228.

[Abderrahim Ihitassen, Younes Belrhazi , Ismail Bettar and Fouad Msanda. (2019); MELISSOPALYNOLOGY AND CLIMATIC VARIATION: CASE OF HONEY ATTRIBUTED TO EUPHORBIA RESINIFERA, REGION OF AZILAL (MOROCCO). Int. J. of Adv. Res. 7 (Jun). 702-725] (ISSN 2320-5407). www.journalijar.com


Abderrahim IHITASSEN
Laboratory of Biotechnologies and Valorization of Natural Resources, Faculty of Sciences, Ibn Zohr University, Agadir Morocco

DOI:


Article DOI: 10.21474/IJAR01/9273      
DOI URL: https://dx.doi.org/10.21474/IJAR01/9273