13Jun 2019

RELATIONSHIP BETWEEN FOLLICULAR FLUID OXIDATIVE STRESS PARAMETER AND THE OUTCOME OF IVF-EMBRYO TRANSFER.

  • Department of Biochemistry, MGM Medical College and Hospital, Aurangabad.
Crossref Cited-by Linking logo

  • Abstract
  • Keywords
  • References
  • Cite This Article as
  • Corresponding Author

Oxidative energy production is unpreventably associated with the generation of reactive oxygen species. Germinal cells cannot be exempt, and may be exposed in the same manner as other cells to the devastating effects of oxygen metabolites. Aim: To study the correlation of oxidative stress marker lipid hydroperoxide in preovulatory follicular fluid of infertile women undergoing in vitro fertilization and their pregnancy outcome. Materials and methods: 60 patients undergoing in vitro fertilization were enrolled in the study. They were divided in two patient groups and compared in terms of follicular fluid lipid hydroperoxides and pregnancy outcome. Result: Pregnant cycles were associated with significantly lower follicular fluid lipid peroxidation (P=0.001). Conclusion: Our results demonstrate that elevated follicular fluid lipid peroxidation level may have a negative impact on In-vitro fertilization outcome. The findings support the idea that level of oxidative stress marker in follicular fluid may play an important role in fertility.

  1. Kamel RM. Management of the infertile couple: an evidence based protocol. Reprod Bio Endocrinol 2010;21(8):1-8.
  2. Mehrotra A, Katiyar DK, Agarwal A, Das V, Pant KK. Role of total antioxidant capacity and lipid peroxidation in fertile and infertile men. Biomedical Research 2013;24(3):347-52.
  3. Das S, Chattopadhyay R, Ghosh S, Goswami SK, Chakraverty BN, Chaudhary K. Reactive oxygen species level in follicular fluid embryo quality marker in IVF? Hum Reprod Advance Access 2006 July;22:1-5.
  4. Revelli A, Piane LD, Casano, Molinari E, Massobrio M, Rinaudo P. Follicular fluid content and oocyte quality: from single biochemical markers to metabolomics. Reprod Biol Endocrino 2009;7:5.
  5. Ruder EH, Hartman TJ, Blumberg J, Goldman MB. Oxidative stress and antioxidants: exposure and impact on female fertility. Human reproduction Update 2008 Jul-Aug;14(4):345-57.
  6. Jozwik M, Wolczynski S, Jozwik M, Szamatowicz M. Oxidative stress markers in preovulatory follicular fluid in humans. Mol Hum Reprod 1999;5:409-13.
  7. World Health Organization WHO. Laboratory manual for the examination of human semen and semen-cervical mucus interaction, 4th Cambridge, UK7C Ambridge University Press, 1999 pp 4-23
  8. Nourooz-Zadeh J, Tajaddini-Sarmadi J, McCarthy S, Betteridge DJ, Wolff SP. Elevated Levels of Authentic Plasma Hydroperoxides in NIDDM. Diabetes 1995;44:1054-58.
  9. Salha O, Nugent D, Kaufmann S, Levett S, Jenner L, Sharma V. The relationship between follicular fluid aspirate volume and oocyte maturity in in-vitro fertilization cycles. Human Reprod 1998;13(7):1901?06.
  10. Carbone MC, Taton C, Monache D, Marci R, Caserta D, Colonna RF. Amicarelli Antioxidant enzymatic defences in human follicular fluid: characterization and age-dependent changes. Molecular Human Reproduction 2003;9(11): 639-43.
  11. Huang JYJ, Rosenwaks Z. In vitro fertilisation treatment and factors affecting success. Best Practice and Research Clinical Obstetrics and Gynaecology 2012;26:777-88.
  12. Das S. Reactive oxygen species level in follicular fluid--embryo quality marker in IVF? Human Reproduction 2006 Jun 3;21(9):2403?7.
  13. Jing LIU, Yanping LI. Effect of oxidative stress and apoptosis in granulosa cells on the outcome of IVF-ET. J Cent South Univ (Med Sci) 2010;35(9):990-94.
  14. Yildirim B, Demir S, Temur I, Erdemir R, Kaleli B. Lipid peroxidation in follicular fluid of women with polycystic ovary syndrome during assisted reproduction cycles. J Reprod Med 2007 Aug; 52(8):722-6.
  15. Bedaiwy MA, Elnashar SA, Goldberg JM, Sharma R, Mascha EJ, Arrigain S et al. Effect of follicular fluid oxidative stress parameters on intracytoplasmic sperm injection outcome. Gynecol Endocrinol 2012 Jan;28(1):51?5.
  16. Yang HW, Hwang KJ, Kwon HC, Kim HS, Choi KW, Oh KS. Detection of reactive oxygen species and apoptosis in human fragmented embryos. Hum Reprod 1998;13:998-1002.
  17. Appasamy M, Jauniaux E, Serhal P, Al-Qahtani A, Groome NP, Muttukrishna A. Evaluation of the relationship between follicular fluid oxidative stress, ovarian hormones and response to gonadotropin stimulation. Fertil Steril 2008;89:912-21.
  18. Paszkowski T, Traub AI, Robinson SY, McMaster D: Selenium dependent glutathione peroxidase activity in human follicular fluid. Clin Chim Acta 1995;236:173-80.
  19. Oral O, Kutlu T, Aksoy E, Fı?ıcıoğlu C, Uslu H, Tuğrul S. The effects of oxidative stress on outcomes of assisted reproductive techniques. J Assist Reprod Genet 2006;23:81-5.
 

[Hina Kausar F.A.Ansari, D.V.Bhale and M.D.Hivre. (2019); RELATIONSHIP BETWEEN FOLLICULAR FLUID OXIDATIVE STRESS PARAMETER AND THE OUTCOME OF IVF-EMBRYO TRANSFER. Int. J. of Adv. Res. 7 (Jun). 386-391] (ISSN 2320-5407). www.journalijar.com

Dr.Hina Kausar F.A.Ansari*
Assistant Professor,Dept of Biochemistry,MGM Medical College,Aurangabad.

DOI:

Article DOI: 10.21474/IJAR01/9235      
DOI URL: https://dx.doi.org/10.21474/IJAR01/9235

Download Full Paper

Download PDF No. of Downloads: 18 | No. of Views: 57