08Jan 2019

COMPARATIVE STUDY OF IMMUNOLOGICAL METHODS FOR THE DIAGNOSIS OF CHLAMYDIA TRACHOMATIS IN WOMEN WITH CERVICITIS AND INFERTILITY.

  • Southern Technical University, Health & Medical Technology College, Basrah/Iraq.
  • Basra Hospital for Women and Children,Basrah /Iraq.
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We have evaluated the diagnostic accuracy of three ELISA tests (IgM, IgG, IgA) and rapid test in detecting C. trachomatisby comparing with direct immunofluorescence (DIF), which is used as a gold standard. Blood samples and endocervical swabs of 100suspectedfemales and 10 healthy women were collected. All sera were tested for chlamydial antibodies of the IgG, IgM and IgA classes by ELISA. Swabs were tested by Rapid test and DIFfor detection of C. trachomatis antigen. Among 100 patients that were included in the study, 46 (46%) women with infertility and 4 (4%) with cervicitis were positive for chlamydia according to the standard method (DIF).None of the healthy women was positive. When compared to DIF results; the ELISA (IgM, IgG, IgA) and rapid test results showed noteworthy differences in the major performance characteristics. The sensitivity of IgM, IgA, IgG and Rapid test was(66, 62, 48, and 24%),specificity (96, 96, 92, and 94%), positive predictive values (94.3, 93.3, 58.7, and 80%),negative predictive values (73.8, 71.6, 63.9, and 55.3%)and Area Under a Curve (0.81, 0.81, 0.7, 0.59.3%) respectively.The results concluded that DIF, IgM, and IgA are valuable techniques to diagnose chlamydial infections. On the other hand, under the conditions of our study, the results suggested that IgG and rapid test are not reliable for diagnosis of chlamydial infections.

  1. WHO guidelines for the treatment of Chlamydia trachomatis; 978 92 4 154971 4 (2016).World Health Organization: WHO Document Production Services, Geneva, Switzerland.
  2. Mohammed, N.S.; AL-Omari, W.R. and AL-Ani. T. A. (1990). Interrelationship of Chlamydia subgroup and other pathogens in the female genital tract. Journal of the Faculty of Medicine. Baghdad,32(3):339.
  3. AL-HusseineiRaad, K.,Khadaem, Khamael M.,AL-Janabi A. Lamya, and FatheiMun'im M. (2009). ELFA and IFAT techniques to detect Chlamydia infection in Baghdad women and It?s effect on theimmunoglobulin level. Journal of the Faculty of Medicine,Baghdad, 51(2), 192-197.
  4. Abdul Karim, E.T., Abdul Muhymen, N. & Al Saadie, M. (‎2009). Chlamydia trachomatis and rubella antibodies in women with full-term deliveries and women with abortion in Baghdad. Eastern Mediterranean Health Journal, 15(‎6)‎, 1407-1411
  5. Al-Hamdani M. Suhad, Shemran A. Haider, Haider S Kadhim and Hassan Yasser (2010). Prevalence of anti-Chlamydial Immunoglobulin in pregnant women. Journal of Collage of Educationl, Iraq (1),95-70.
  6. Ahmed T. Shatha(2012). Detection of Chlamydia trachomatis using Polymerase Chain Reaction PCR. Baghdad University /Science Collage for women /Biology Department.Al- Mustansiriyah Journal of Science,23(6), 35-42.
  7. Tahir, M. A. (2016). Molecular and serological detection of Chlamydia trachomatis among infertile women in Basra (Unpublished master's thesis). University of Basrah, Medicine College, Basrah.
  8. Price, M. J., Ades, A. E., Welton, N. J., Simms, I., Macleod, J., & Horner, P. J. (2016). Proportion of Pelvic Inflammatory Disease Cases Caused by Chlamydia trachomatis: Consistent Picture From Different Methods. Journal of Infectious Diseases, 214(4), 617-624.
  9. Lanjouw, E., Ossewaarde, J. M., Stary, A., Boag, F., & Van der Meijden, W. I. (2010). 2010 European guideline for the management of Chlamydia trachomatis infections. International Journal of STD & AIDS, 21(11), 729-737.
  10. Morre, S. A., 1 l. Rozendaal,1 i. G. M. Van valkengoed,2 a. J. P. Boeke,2 p. C. Van voorst vader,3 j. Schirm, s. De blok,5 j. A. R. Van den hoek,6 g. J. J. Van doornum,6 c. J. L. M. Meijer,1 and a. J. C. Van den brule1 (2000).Urogenital Chlamydia trachomatis Serovars in Men and Women with a Symptomatic or Asymptomatic Infection: an Association with Clinical Manifestations?.JOURNAL OF CLINICAL MICROBIOLOGY, 38(6), 2292?2296
  11. Barnes, R. C. (1989). Laboratory diagnosis of human chlamydial infections. Clinical Microbiology Reviews, 2(2), 119-136.
  12. Pi?mont y., jaulhac b. (1995): use of molecular methods for bacteriology diagnosis. Annales de dermatologieet de v?n?r?ologie, 122, 206-12.
  13. Osewaarde JM, Rieffe M, Rozenberg-Arska M, et al. (1992): Development and clinical evaluation of polymerase chain reaction tests for detection of Chlamydia trachomatis. Journal of ClinicalMicrobiology,30(8), 2122-2128.
  14. Crotchfelt, K.A. Welesh,L.E. Debonville,D. Rosenstraus, M. Quinn,T.C.(1997). Detection of Neisseria gonorrhoeaeand Chlamydia trachomatis in genitourinary specimens from men and women by a coamplification PCR assay. Journal of Clinical Microbiology,35(6),1536-40.
  15. Baud, D., Regan, L., &Greub, G. (2010). Comparison of five commercial serological tests for the detection of anti-Chlamydia trachomatis antibodies. European Journal of Clinical Microbiology & Infectious Diseases, 29(6), 669-675.
  16. Shulman, L. (2012). Chlamydia trachomatis Antigens Recognized in Women With Tubal Factor Infertility, Normal Fertility, and Acute Infection. Yearbook of Obstetrics, Gynecology and Women's Health, 2012, 305-306.
  17. Halvorsen, D. S., Borvik, T., Njolstad, I., Gutteberg, T. J., Vorland, L. H., & Hansen, J. (2002). Chlamydia pneumoniae IgA- and IgG antibodies in young survivors of myocardial infarction. A comparison of antibody detection by a microimmunofluorescence test and an enzyme immunoassay. Journal of Internal Medicine, 251(2), 142-147.
  18. Jones, C. S. (2003). Measurement of IgG antibodies to Chlamydia trachomatis by commercial enzyme immunoassays and immunofluorescence in sera from pregnant women and patients with infertility, pelvic inflammatory disease, ectopic pregnancy, and laboratory diagnosed Chlamydia psittaci/Chlamydia pneumoniae infection. Journal of Clinical Pathology, 56(3), 225-229.
  19. Moss, T. R. &Darougar, S. (2001). Human genital infections with Chlamydia trachomatis ? is there a role for serology? In: International handbook of Chlamydia [ed. Moss, T. R.] pp 33 ? 47. Euromed Publications Ltd., Haslemere UK, ISBN 1 899015 43 4
  20. OSTERGAARD, L. (1999). Diagnosis of urogenital Chlamydia trachomatis infection by use of DNA
  21. APMIS, 107(S89), 5-36.
  22. Hadgu, A. (1996). The discrepancy in discrepant analysis. The Lancet, 348(9027), 592-593.
  23. Tanaka, M., Nakayama, H., Yoshida, H., Takahashi, K., Nagafuji, T., Hagiwara, T., &Kumazawa, J.
  24. (1998). Detection of Chlamydia trachomatis in vaginal specimens from female commercial sex workers
  25. using a newimproved enzyme immunoassay. Sexually Transmitted Infections, 74(6), 435-438.
  26. Meyer, T. (2016). Diagnostic Procedures to Detect Chlamydia trachomatisInfections. Microorganisms, 4(3), 25.
  27. Ivo, D. (2006). Introduction to statistical methods for the life and health sciences.Uinversity of Califorina Los Angels.Http://www.stat.ucla.edu/-dinov/courses-students.
  28. Verkooyen, R. P., Peeters, M. F., Van Rijsoort-Vos, J. H., Van Der Meijden, W. I., & Mouton, J. W. (2002). Sensitivity and specificity of three new commercially available Chlamydia trachomatis tests. International Journal of STD & AIDS, 13(1_suppl), 23-25.
  29. Chernesky, M. A., Mahony, J. B., Castriciano, S., Mores, M., Stewart, I. O., Landis, S. J., and Leman, C. (1986). Detection of Chlamydia trachomatis Antigens by Enzyme Immunoassay and Immunofluorescence in Genital Specimens from Symptomatic and Asymptomatic Men and Women. Journal of Infectious Diseases, 154(1), 141-148.
  30. Rapoza, P. A. (1986). Assessment of neonatal conjunctivitis with a direct immunofluorescent monoclonal antibody stain for Chlamydia. JAMA: The Journal of the American Medical Association, 255(24), 3369-3373.
  31. Smith, J. W., R. E. Rogers, B. P. Katz, J. F. Brickler, P. L. Lineback, B. VanderPol, and R. B. Jones. (1987). Diagnosis of chlamydial infection in women attending antenatal and gynecologic clinics. Journal of Clinical Microbiology, 25(5), 868?872.
  32. Carolyn, M. B. (1997) Current Methods of Laboratory Diagnosis of C.trachomatisInfections.Clinical
  33. Microbiology reviews, 10(1):160-184.
  34. Thejls, H., Gnarpe, J., Gnarpe, H., Larsson, P. G., Platz-Christensen, J. J., Ostergaard, L., & Victor, A. (1994). Expanded gold standard in the diagnosis of Chlamydia trachomatis in a low prevalence population: diagnostic efficacy of tissue culture, direct immunofluorescence, enzyme immunoassay, PCR and serology. Sexually Transmitted Infections, 70(5), 300-303.
  35. Mohammed, H. Iqbal, Al-Awadei, J. Salwa, Saadedin, M.K. Shurook. (2017). Molecular diagnosis ofChlamydia trachomatis in infertile women? using Real time PCR and comprise with other methods.Iraqi Journal of science,58(3B), 1437-1446.
  36. Nu?ez-Forero, L., Moyano-Ariza, L., Gait?n-Duarte, H., ?ngel-M?ller, E., Ruiz-Parra, A., Gonz?lez, P.,Tolosa, J. E. (2015). Diagnostic accuracy of rapid tests for sexually transmitted infections in symptomatic women. Sexually Transmitted Infections, 92(1), 24-28.
  37. Centers for Disease Control and Prevention. Screening Tests to Detect Chlamydia trachomatis and Neisseria gonorrhoeae Infections ? 2002. MMWR 2002;51(No. RR-15): [page 8].

[Abrar A. H. Sahi, Khairallah A.S. Mohammed and Zainab Y. Al Salman. (2019); COMPARATIVE STUDY OF IMMUNOLOGICAL METHODS FOR THE DIAGNOSIS OF CHLAMYDIA TRACHOMATIS IN WOMEN WITH CERVICITIS AND INFERTILITY. Int. J. of Adv. Res. 7 (Jan). 528-534] (ISSN 2320-5407). www.journalijar.com

Assist Prof. Dr Khairallah A S Mohammed
Southern Technical University, Health & Medical Technology College, Basrah/Iraq

DOI:

Article DOI: 10.21474/IJAR01/8358      
DOI URL: https://dx.doi.org/10.21474/IJAR01/8358

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