06Mar 2019

ETUDE PHYTOCHIMIQUE ET ACTIVITES BIOLOGIQUES DE DEUX PLANTES : ALCHORNEA FLORIBUNDA (EUPHORBIACEAE) ET BRIDELIA FERRUGINEA (PHYLLANTHACEAE).

  • Laboratoire de Physiologie animale et Pharmacologie, Unit? de recherche Agrobiologie, Universit? des Sciences et Techniques de Masuku (USTM), BP 067 Franceville, Gabon.
  • Laboratoire de Biologie Moleculaire et Cellulaire, equipe de Bacteriologie-Immunologie, Unite de recherche Agrobiologie, Universite des Sciences et Techniques de Masuku (USTM), BP 067 Franceville, Gabon.
  • Laboratoire de Physiologie vegetale, Phytopathologie et Amelioration des plantes, Unite de recherche Agrobiologie, Universite des Sciences et Techniques de Masuku (USTM), BP 067 Franceville, Gabon.
Crossref Cited-by Linking logo

  • Abstract
  • Keywords
  • References
  • Cite This Article as
  • Corresponding Author

In addition to metabolic diseases, microbial pathologies often characterized by resistance and multiresistance phenomena of certain strains with known reference molecules, constitute a real public health problem in the world as a result of the number of deaths caused each year by these pathologies. The purpose of this work is to evaluate the antioxidant and antimicrobial potential of aqueous extracts of Alchornea floribunda and Brideliaferruginea. The phytochemical composition of the extracts was determined by the calorimetric and differential method. The antioxidant activity was evaluated by the antiradical activity of the radical DPPH. Also, antimicrobial activity was determined by the well method in agar and determination of minimum inhibitory (MIC) and bactericidal (MBC) concentrations. The results of the phytochemical screening reveal a wealth of phytomolecules with antimicrobial properties such as polyphenols, terpenes, flavonoids, tannins and alkaloids. Also, they emphasize that both extracts have antioxidant activities. However, the average antioxidant activity of the extract of Alchornea floribunda is greater than that of Brideliaferruginea. This activity is comparable to that of gallic acid and ascorbic acid in the DPPH free radical scavenging assay. The study of the antimicrobial activity of these two plants revealed a significant antibacterial activity with respect to several bacterial strains responsible for infantile diarrhea, namely: Shigellasonnei, Shigella spp., Yersinia pestis, Raoultellaornithinilytica, Escherichia vulneris, Salmonella choleraesuis, Escherichia coli, as well as a remarkable yeast antifungal activity (Candidaspp PV, Candida albicansbucal, Candida albicans PV).

  1. Alsarhan, A., Mansi, K., Aburjai, T., Al-khatib, A., Alzweiri, M., and Sultana, N. (2013). HSP70 and inos biomarkers in evaluating the healing properties of rubiatinctorum. European Scientific Journal, 9(15), 241?254.
  2. Batawila, K. (2002). Diversit?, ?cologie et propri?t?s antifongiques des Combretaceaea du Togo. Acta Bot. Gallica. Univ. Reims et UnivLom?.
  3. Bidi?, A. P., Koffi, E., N?guessan, J. D., Djaman, A. J., and Gu?d?-Guina, F. (2008). Influence of Mitragynaciliata (Myta) on the microsomalactivity of ATPase Na+/ K+ dependent extract on a rabbit (heart) African. Int. J. Biomol. Biomed., 5, 294?301.
  4. Bouquet, A., and Fouret, A. (1975). Recherches chimiques pr?liminaires sur les plantes m?dicinales du Congo-Brazzaville. Fitoterapia, 4, 175?177.
  5. Cardona, F., Andr?s-lacueva, C., Tulipani, S., Tinahones, F., and Queipo-ortu?o, M. (2013). Benefits of polyphenols on gut microbiota and implications in human health. The Journal of Nutritional Biochemistry, 24(8), 1415?1422. https://doi.org/10.1016/j.jnutbio.2013.05.001
  6. EyiMintsa, A. B. (2017). Etude des propri?t?s pharmacologiques et physiologique de Helichrysummechowianum (Asteriaceae), plante utilis?e par les pygm?es du Gabon. Th?se. Universit? des Sciences et Techniques de Masuku (Gabon).
  7. Falconer, S. B., and Brown, E. D. (2009). New screens and targets in antibacterial drug discovery. Current Opinion in Microbiology, 12(5), 497?504.
  8. Gruber, J., Schaffer, S., and Barry, H. (2008). The mitochondrial free radical theory of ageing ? Where do we stand? Frontiers in Bioscience, 13, 6554?6579.
  9. Guillot, J., and Dannaoui, E. (2015). La r?sistance aux antifongiques : importance en m?decine humaine et v?rit?rinaire. Acad. V?t. France, Tome 168(4), 314?319.
  10. Hsu, C. Y., Chan, Y. P., and Chang, J. (2007). Antioxidant activity of extract from Polygonum. Biol Res, 40, 13?21.
  11. Huffman, M. A. (2003). Animal self-medication and ethno-medecine: exploration and exploitation of the medication properties of plants. Proceeding of the NutrSoc, 62, 371?381.
  12. Kamurthy, H., Tejmal, M., Majumder, P., and Ambujakshi, H. R. (2016). Antifungal Activity of Weed Extracts on Candida albicans. An In-vitro Study. International Journal of Phytomedicine, 8, 453?456. https://doi.org/10.5138/09750185.1847
  13. Konat?, K., Souza, A., KassiYomalan, T., Dibala, I. C., and Barro, N. (2011). Phytochemical composition, Antioxidant and Anti-inflammatory potential of bioactive fractions from extracts of three medicinal plants traditionally used to treat liver diseases in Burkina Faso. International Journal of Phytomedicine, 3, 406?415.
  14. KporouKouassi, E., KraAdouKoffi, M., OUattara, S., and Guede-Guina, F. (2009). ?valuation de la sensibilit? de Candida albicans aux extraits de Mitracarpusscaber une Rubiaceae codifi?e Misca. Bulletin de La Soci?t? Royale Des Sciences de Li?ge, 78, 12?23.
  15. Lahouel, M., Boulkour, S., Segueni, N., and Fillastre, J. P. (2004). du cyclophosphamide et du parac?tamol par inhibition de la peroxydationlipidique et augmentation du glutathionh?patique The flavonoids effect against vinblastine, cyclophosphamide and paracetamol toxicity by inhibition of lipid-peroxydation and increas. PathologieBiologie, 52, 314?322. https://doi.org/10.1016/j.patbio.2004.01.001
  16. Devers, D., Vandeweghe, J.P., Billand, A., Cassagne, B., Doucet, J.L., Nasi, R., Tr?fon, T., and Tutin, C. (2006). Les for?ts du Bassin du Congo :Etat des For?ts 2006.
  17. Li, H., Wang, X., Li, P., Li, Y., and Wang, H. (2008). Comparative Study of Antioxidant Activity of Grape (Vitisvinifera ) Seed Powder Assessed by Different Methods. Journal of Food and Drug Analysis, 16(6), 1?8.
  18. (2013). Structures-Activit?s biologiques des polysaccharides de quelques plantes end?miques du Gabon. Th?se, 243 p.
  19. Merghache, D., Boucherit, A. Z., and Boucherit, K. (2012). ?valuation de l?activit? antifongique de diff?rents extraits de la cannelle de Chine (Cinnamomum cassia). Phytoth?rapie, 10(4), 215?221.
  20. Merghache, D., Boucherit, A. Z., and Boucherit, K. (2012). Evaluation de l?activit? antifongique des diff?rents extraits de la cannelle de Chine (Cinnamomum cassia). Phyto, 10(4), 215?221.
  21. Moroh, J. A., Bahi, C., Dje, K., and Loukou, Y. G. (2008). ?tude de l ?activit?antibact?rienne de l ? extraitac?tatique (EAC) de Morindamorindoides (Baker) milne-redheat (rubiaceae) sur la croissance in-vitro des souchesd?Escherichia coli Study of the antibacterial activity of Morindamorindoides (Baker). Bulletin de La Soci?t? Royale Des Sciences de Li?ge, 77, 44?61.
  22. MusuyuMuganza, D., Fruth, B. I., NzunzuLami, J., Mesia, G., Kambu, O. K., Tona, G., and Pieters, L. (2012). In vitro antiprotozoal and cytotoxic activity of 33 ethonopharmacologically selected medicinal plants from Democratic Republic of Congo. J Ethnopharmacol., 141(1), 301?8.
  23. NsiAkou?, G. (2017). Etude des plantes m?dicinales et/ou alimentaires issues du r?gime alimentaire du mandrill (Mandrillus sphinx) : Approche Zoopharmacognosie. Th?se. Universit? Des Sciences et Techniques de Masuku (Gabon), 176 pages.
  24. NsiAKou?, G., Obame, L. C., ONDO, J., Brama, I., Mbadinga-Mbading, W., OtogoN?nang, E. S., and Mbatchi, B. (2013). phytochemical and antiradical activities of Agelaeapentagyna (Lam) Baill and Dialiumdinklagei Harms. Medicinal plants from Gabon. International Journal of Advanced Research, 1(8), 246?255.
  25. NsiAkou?, G., Obame, L. C., Ondo, J. P., Brama, I., OtogoN?Nang, E. S., Tapoyo, S. Y., and Souza, A. (2013). Phytochemical composition and antiradical activity of Sakersiaafricana Hook. f. medicinal plant from Gabon Sakersia total. Int. J. Biomol. Biomed. Introduction, 3(3), 1?8.
  26. Ochi, I. O., Ogah, E., Longbap, B. D., Abiaziem, C. V., and Tabe, N. T. (2015). PHYTOCHEMICAL ANALYSIS AND ANTIMICROBIAL SCREENING OF DRIED ROOT. Ewemen Journal of Microbial Research 2015, 1(1), 25?30.
  27. Okoye, E. L., Uba, B. O., Uhobo, P. C., Oli, A. N., and Ikegbunam, M. N. (2014). Evaluation of the Antibacterial Activity of Methanol and Chloroform Extracts of Alchorneacordifolia Leaves. Journal of Scientific Research & Reports, 3(June 2012), 255?262.
  28. (2002). Strat?gie de l?OMS pour la m?decine traditionnelle pour 2002-2005. WHO/EDM/TRM.
  29. (2011). Statistiques sanitaires mondiales. OMS.
  30. (2013). Les maladies diarrh?iques : Aide-m?moire.
  31. Ondo, J. P. (2011). Etude phytochimique et ?valuation de l?activit? antiplasmodiale de Vitex madiensisOliv. (Lamiaceae) et de Croton mayumbensis (Euphorbiaceae), plantes m?dicinales gabonaises. Th?se. Universit? de Rouen.
  32. Ondo, P. J., Obame, L. C., AndziBarh?, T., NsiAkoue, G., and NsiEmvo, E. (2013). Phytochemical screening , total phenolic content and antiradical activity of Aspleniumafricanum ( Aspleniaceae ) and fruit of Megaphriniummacrostachyum (Marantaceae). Journal of Applied Pharmaceutical Science, 3(8), 92?96. https://doi.org/10.7324/JAPS.2013.3816
  33. Paris, R., and Moyse, H. (1969). Pr?cis de mati?re m?dicinale. In Paris : Masson (pp. 205?208).
  34. Pincemail, J., Degrune, F., Voussure, S., and Malherbe, C. (2007). Effet d?une alimentation riche en fruits et l?gumes sur les taux plasmatiques en antioxydants et des marqueurs des dommages oxydatifs Effect of a dietrich in fruits and vegetables on the plasmaticantioxidant rates and of the markers of the oxidative d. Nutrition Clinique et M?tabolisme, 21, 66?75. https://doi.org/10.1016/j.nupar.2007.04.005
  35. Scherer, R., and Godoy, H. T. (2009). Antioxidant activity index (AAI) by 2,2-diphenyl-1- picrylhydrazyl method. Food Chem, 112, 654?658.
  36. Shoji, T., Akazome, Y., Kanda, T., and Ikeda, M. (2004). The toxicology and safety of apple polyphenol extract. Food and Chemical Toxicology, 42, 959?967. https://doi.org/10.1016/j.fct.2004.02.008
  37. Tene, M., Ndontsa, B. L., Tane, P., Tamokou, J. D. D., and Kuiate, J. R. (2009). Antimicrobial diterpenoids and triterpenoids from the stem bark of Croton macrostachys. Int. J. Biol. Chem. Sci, 3(3), 538?544.
  38. Yala, J. F., MabikaMabika, R., Camara, B., Tuo, S., Souza, A., Lepengue, N. A., and Mbatchi, B. (2017). Assessment of the antibacterial activity of four essential oils and the. International Journal of Phytomedicine, 9(3), 443?450. https://doi.org/10.5138/09750185.2106
  39. Yala, J. F., Ntsameso-Mve-Mba, V., AzzizetIssembe, Y., Lepengue, A. N., and Souza, A. (2016). ?valuation in vitro de l ? activit? antimicrobienne de l ? extrait aqueux d ? Eryngiumfoetidium r?colt? dans la ville de Franceville . Journal of Applied Biosciences, 103, 9886?9893.
  40. Yang, Y. L., Li, S. Y., Cheng, H. H., and Lo, H. J. (2005). Susceptibilities to amphotericin B and fluconazole of Candida species in TSARY 2002. DiagnMicrobiol Infect Dis, 51(3), 179?83.
  41. Zongo, C., Savadoga, A., Ouattara, L., Bassole, H. N., Ouattara, C. A. T., Ouattar, A. S., and Traore, A. . (2010). Polyphenols Content, Antioxydant and Antimicrobial activities of Ampelocissusgrantii (Baker) Planch. (Vitaceae): A Medicinal plant from Burkina Faso. IntJof Pharm, 6(6), 880?887.

[Boris Achille Eyi Mintsa, Ibrahim, Rolande Mabika Mabika, Wenceslace Landry Mve Mendame, Alexis Nicaise Lepengue, Alain Souza and Jean Fabrice Yala. (2019); ETUDE PHYTOCHIMIQUE ET ACTIVITES BIOLOGIQUES DE DEUX PLANTES : ALCHORNEA FLORIBUNDA (EUPHORBIACEAE) ET BRIDELIA FERRUGINEA (PHYLLANTHACEAE). Int. J. of Adv. Res. 7 (Mar). 331-345] (ISSN 2320-5407). www.journalijar.com

Jean Fabrice YALA
Laboratoire de Biologie Moléculaire et Cellulaire, équipe de Bactériologie-Immunologie, Unité de recherche Agrobiologie, Université des Sciences et Techniques de Masuku (USTM), BP 067 Franceville, Gabon.

DOI:

Article DOI: 10.21474/IJAR01/8639      
DOI URL: https://dx.doi.org/10.21474/IJAR01/8639

Download Full Paper

Download PDF No. of Downloads: 43 | No. of Views: 171