22Dec 2018

PHYTOCHEMICAL ANALYSIS AND EVALUATION OF ANTIBACTERIAL ACTIVITY OF TERMINALIA CHEBULA, MOMORDICA CHARANTIA, DREGEA VOLUBILIS PLANT EXTRACTS.

  • Department of Biotechnology, Chaitanya Postgraduate College (Autonomous), Kishanpura, Hanamkonda-506001 TS-India.
  • Natural Products Laboratory, Chaitanya Postgraduate College (Autonomous), Kishanpura, Hanamkonda-506001-TS-India.
  • Department of Biochemistry and Bioinformatics, GitamUniversity,Rushikonda, Visakhapatnam, AP-India.
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The preset study was framed out to evaluate the phytochemicals and the antibacterial activity of the fractions separated from crude extracts of the Terminaliachebula, Momordicacharantia, Dregeavolubilis.Methods: Phytochemical analysis was carried out using standard such as Mayer?s test, Wagner?s test, Dragendorff test, Hager?s Test, Alkaline copper test, Lead acetate test, Ferric chloride (FeCl3) test to identify the presence of secondary metabolites such as alkaloids, Flavonoids, Tannins in the fractions separated from the above selected plants. Results: The test carried out for screening of phytochemicals was given positive. According to results we noticed the presence of different types of secondary metabolites such as Alkaloids, Phenols, Flavanoids, Tanins, Saponins, Carbohydrates, Amino acids in the leaf fractions. The fractions inhibited the growth of bacterial strains used in the study and exhibited antibacterial activity. The results were compared with standard reference drug ciprofloxacin at 10 mg/mL. Based on our results, it is noted that, the chloroform, ethanol and aqueous fractions are more active than n-hexane, toluene fractions. The least MIC value (12 and 16?g/ml) was recorded with ethanol and aqueous fractions of Momordicacharantia respectively. Conclusion: fractions of separated from theDregeavolubilis, Terminaliachebula, Momordicacharantiamedicinal plants (leaves) had significant phtochemicals which are responsible for antimicrobial activity.

  1. Beloin, N., Gbeassor, M., Akpagana, K., Hudson, J., de Soussa, K., Koumaglo, K., Ethnomedicinal uses of Momordicacharantia (Cucurbitaceae) in Togo and relation to its phytochemistry and biological activity. J Ethnopharmacol 2005; 96: 49-55.
  2. Conner, DE (1993): Naturally occurring compounds. In Antimicobials in foods, Davidson, P.T. and Branen, A.L. (Eds.). Marecl Dekker, New York, pp. 441-468.
  3. Cowan, MM (1999): Plant products as antimicrobial agents. Clin. Microbiol. Rev., 12(4): 564-582.
  4. Das, K., Tiwari, RKS.,Shrivastava, DK (2010): Techniques for evaluation of medicinal plant products as antimicrobial agent: Current methods and future trends. Journal of Medicinal Plants Research., 4(2): 104-111.
  5. Duke, JA (1985): Handbook of medicinal herbs. CRC Press, Boca Raton FL 1985; 315-316. 10.
  6. Grover, JK. andYadav, SP (2004): Pharmacological actions and potential uses of Momordicacharantia. A Rev J Ethnopharmacol., 93(1): 123-132.
  7. Harbone, JB (1998): Phytochemical methods: Aguide to modern techniques of plant analysis. 2nd London: Champman and Hall; pp 54-84.
  8. Kannan, P., Ramadevi, SR., Hopper, W (2009): Antibacterial activity of Terminaliachebula fruit extract. Afri J Microbiology Research., 3(4):180-184.
  9. Khan, MU.,HabibullahKhalilullah., Jawed Akhtar., Gamal Osman Elhasan (2015): TerminaliaChebula: An Ephemeral Glance. Int J Pharm Pharm Sci., 7(2): 40-43.
  10. Kim, TG., Kang, SY., Jung, KK., Kang, JH., Lee, E., Han, HM., Kim, SH (2001): Antiviral activities of extracts isolated from Terminalischebula Retz., Sanguisorbaofficinalis L., RubuscoreanusMiq and Rheum palmatum L. against hepatitis B virus. Phyto res.,15(8): 718-720.
  11. Kim, J., Marshal, MR., Wei, C (1995): Antibacterial activity of some essential oil components against five food borne pathogens. J. Agric. Food Chem., 43(11): 2839-2845.
  12. Kokate, KC (1997): 4th Delhi: VallabhPrakashan; Practical Pharmacognosy; p.218
  13. Kumar, H. and Totawar, P (2015): Rationale behind Uses of Medicinal Plants by Gond Tribe of Sironcha Tehsil, District- Gadchiroli, M.S., India. Int. J. Res Biosci, Agriculture and technology, (Special issue 1): 43-47.
  14. Marcucci, MC.,Ferreres, F., Viguera, C., Bankova, VS., Castro, SL., Dantas, AP.(2001):Phenolic compounds from Brazilian propolis with pharmacological activities. J. Ethnopharmacol., 74(2): 105-112.
  15. Ng, TB., Chan, WY., Yeung, HW (1992): Proteins with abortifacient, ribosome inactivating, immunomodulatory, antitumor and anti-AIDS activities from Cucurbitaceae plants. J. Pharmacol., 23(4): 579-590.
  16. Nikhal, SB., Dambe, PA., Ghongade, DB.,Goupale, DC (2010): Hydroalcoholic extraction of Mangiferaindica (leaves) by Soxhletion. Int J Pharm Sci., 1(7) : 30-32
  17. Nikaido, H (1996): Outer membrane. In Escherichia coli and Salmonella: Cellular and Molecular Biology, Neidhardt, F.C. (Eds.). ASM Press, Washington DC, pp. 29-47.
  18. Pandikumar, P., Ayyanar, M., Ignacimuthu, S (2007): Medicinal plants used by?Malasartribes of Coimbatore district, Tamil Nadu.?Indian J Trad Knowledge.?6(4):579?582.
  19. Prakash Chandra, G (2012): Biological and Pharmacological properties of Terminaliachebula (haritaki)- an overview. Int. J. Pharm Pharm Sci., 4(3): 62-68.
  20. Rajadurai, M., Vidhya, VG.,Ramya, M (2009): Ethno-medicinal plants used by the traditional healers of Pacchamalai hills, Tamil Nadu, India.?J EthnobiolEthnomed.,?; 3(1):39?41.
  21. Rao, NK. andNammi, S (2006): Antidiabetic and renoprotective effects of the chloroform extract of Terminaliachebula Retz. Seeds in streptozotocin-induced diabetic rats. BMC Complement Altern Med., 6: 17.
  22. Scartezzini, P. and Speroni, E (2000): Review on some plants of Indian traditional medicine with antioxidant activity. J Ethnopharmacol., 71(1-2): 23-43.
  23. Silija, VP.,SamithaVarma, K., Mohanan, KV (2008): Ethnomedicinal plant knowledge of the Mullukuruma tribe of Wayanad district, Kerala.?Indian J TradKnowledge.,?7 (4):612?614.
  24. Suchalatha, S., Shyamala Devi CS (2004): Protective effect of Terminaliachebula against experimental myocardial injury induced by isoproterenol. Indian J Exp Biol., 42(2): 174-78.
  25. Vaibhav, A. and Arun Kumar, W. (2011): Immunomodulatory Activity of Alcohol Extract of Terminaliachebula Retz Combretaceae. Tropical Journal of Pharmaceutical Research., 10(5): 567-575.
  26. Pandey, R. and Kalra, A. (2003): Root knot disease of ashwagandhaWithaniasomnifera and its ecofriendly cost effective management. J. Mycol. Pl. Pathol., 33(2): 240-245.

[Thupurani Murali Krishna, Urmila Bonkuri, Racha Srikanth, Challa Surekha, Peddoju Pranay and Venkalapally Thirupathaiah. (2018); PHYTOCHEMICAL ANALYSIS AND EVALUATION OF ANTIBACTERIAL ACTIVITY OF TERMINALIA CHEBULA, MOMORDICA CHARANTIA, DREGEA VOLUBILIS PLANT EXTRACTS. Int. J. of Adv. Res. 6 (Dec). 1195-1201] (ISSN 2320-5407). www.journalijar.com

Thupurani Murali Krishna
Department of Biotechnology, Chaitanya Postgraduate College (Autonomous), Kishanpura, Hanamkonda-506001-TS-India.

DOI:

Article DOI: 10.21474/IJAR01/8252      
DOI URL: https://dx.doi.org/10.21474/IJAR01/8252

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