17Sep 2018

COMPARATIVE STUDY OF THE ANTIBACTERIAL ACTIVITY OF CINNAMON AND ORIGAN ESSENTIAL OILS AND THEIR PRIMARYCOMPONENTS ON AVIAN Escherichia coli STRAINS.

  • Department of Biological and Pharmaceutical Sciences, Hassan II Agronomic and Veterinary Institute, BP 6202, 1010, Rabat, Morocco.
  • Department of Food and Nutrition Sciences, Agronomic and Veterinary Institute Hassan II, BP 6202, Rabat, Morocco.
  • Avian Pathology Unit, Department of Pathology and Veterinary Public Health, Agronomic and Veterinary Institute Hassan II, BP 6202, Rabat- Institute, Rabat, Morocco.
Crossref Cited-by Linking logo

  • Abstract
  • Keywords
  • References
  • Cite This Article as
  • Corresponding Author

The emergence of bacterial resistance to the usual antimicrobial agents is nowadays a major challenge in therapeutics for both humans and animals. As a result, the search for new antimicrobial agents has become a necessity. In this context, a great interest was focused on naturally occurring substances with antibacterial activity. The present study consists of evaluating the antibacterial activity of two essential oils (EO) from two aromatic and medicinal plants commonly used in humans in Morocco, namely, oregano (origanum vulgare) and cinnamon (Cinnamomum cassia), and their primarycomponents, carvacrol and cinnamaldehyde respectively, against antibiotic-resistantEscherichia coli strains of avian origin. Extraction of the EO was carried out by hydrodistillation, and their chemical analysis was carried out by gas chromatography (GC).The cinnamon EO is mainly composed of cinnamaldehyde (nearly 90%), followed by beta-caryophyllen (6.1%), cinnamyl acetate and eugenol with (2.75%) and (0.46%); carvacrol is the primary component of oregano EO (65.51%), followed by gamma-terpinene (11.49%), p-cymene (6.27%), thymol (4%) and borneol (3.58%). The aromatogram showed interesting antibacterial activity of EO and their active ingredients against the used bacterial strains. The diffusion method on agar medium showedthat EO of cinnamon was more active than oregano EO against E. colistrains, with mean inhibition zones of 25.8+4.5mm and 17+3.7 mm, respectively. The obtained MICs showed also that cinnamaldehyde was more potent than carvacrol, with average MICs of 0.039% and 0.106%, respectively.

  1. Bouhdid S., Skali S.N., Idaomar M., Zhiri A., Baudoux D., Amensour M., and Abrini J., (2008). Antibacterial and antioxidant activities of Origanum compactum essential oil. African Journal of Biotechnology Vol. 7 (10), pp. 1563-1570, 16 May. Available online at http://www.academicjournals.org/AJB ISSN 1684?5315.
  2. Bouhdid S., (2009). Activit?s antimicrobienne et antioxydante des huiles essentielles. Application biotechnologique pour l?am?lioration de la qualit? des boyaux naturels. Th?se de Doctorat en Sciences (Sp?cialit? : Biotechnologie, Discipline : Microbiologie). Universit? Abdelmalek essaadi. Facult? des Sciences.
  3. Bouhdid S., Abrini2 J., Baudoux D., Manresa A., Zhiri A., (2012). Essential oils of oregano compact and cinnamon: antibacterial potency and mechanism of action. J Pharm Clin 2012; 31 (3) : 141-8.
  4. Bounatirou S., Smiti S., Miguel M.G., Rejeb M.N., Nefati M., Costa M.M., Faleiro L., Figueiredo A.C., Baroso J.G., et Pedro L.G., (2007). Chemical composition, antioxidant and antibacterial activities of the essential oils isolated from Tunisian Thymus capitatus Hoff. et Link. Food Chemistry, 105, 146 Ŕ 155.
  5. Cao L., Yong Si J., Liu Y., Sun H., Jin W., LiZ., Hong ZhaoX., and Le Pan R., (2009). Essential oil composition, antimicrobial and antioxidant properties of Moslachinensis(Maxim). Food Chem., 115, 3801 Ŕ 805.
  6. Chorianopoulos N., Kalpoutzakis E., Aligiannis N., Mitaku S., Nychas G.J., et Haroutounian S.A., (2004). Essential oils of Satureja, Origanum, and Thymus species: Chemical composition and antibacterial activities against foodborne pathogens. J. of Agric. and Food Chem., 52, 8261 Ŕ 8267.
  7. Clevenger J.F., (1928). Apparatus for the determination of volatile oil. Am. Pharm. Assoc., 17(4), 346-351.
  8. Dorman H. J. D., Deans S.G., (2000). Antimicrobial agents from plants: antibacterial activity of plant volatile oils. Journal of Applied Microbiology 88, 308-316
  9. Elgayyar M., Draughon F.A., Golden D.A., Mount J.R., (2001). Antimicrobial activity of essential oils from plants against selected pathogenic and saprophytic microorganisms. Journal of Food Protection 64(7), 1019-1024.
  10. Fabio A., Corona A., Forte E., and Quaglio P., (2003). Inhibitory activity of spices and essential oils on psychrotrophic bacteria. Microbiol., 26(1): 115-120.
  11. Friedman M?., Henika P.R., Mandrell R.E., (2002). Bactericidal activities of plant essential oils and some of their isolated constituents against Campylobacter jejuni, Escherichia coli, Listeria monocytogenes, and Salmonella enterica. J Food Prot. 65:1545-60.
  12. Gill A.O., Delaquis P., Russo P., et Holley R.A., (2002). Evaluation of antilisterial action of cilantro oil on vacuum packed ham. Int. J. of Food Microbiology, 73, 83 Ŕ 92.
  13. Hammer K. A., Carson C. F., Riley T.V., (1999). Antimicrobial activity of essential oils and other plant extracts. Journal of Applied Microbiology 86, 985-990.
  14. Hammou F., Skali S.N., Idaomar M., and Abrini J., (2011). The antimicrobial effect of Origanum compactum essential oil, nisin and their combination against Escherichia coli in tryptic soy broth (TSB) and in sheep natural sausage casings during storage at 25 and 7?C.? African Journal of Biotechnology Vol. 10(71), pp. 15998-16005, 14 November. Available online at http://www.academicjournals.org/AJB DOI: 10.5897/AJB11.1046
  15. Hawkins, Savannah G., (2014). ?Antimicrobial Activity of Cinnamic Acid, Citric Acid, Cinnamaldehyde, and Levulinic Acid Against Foodborne Pathogens?. University of Tennessee Honors Thesis Projects. http://trace.tennessee.edu/utk_chanhonoproj/1701.
  16. Inouye S., Takizawa T., Yamaguchi H., (2001). Antibacterial activity of essential oils and their major constituents against respiratory tract pathogens by gaseous contact. J AntimicrobChemother; 47:565-73.
  17. Kalemba D., et Kunicka A., (2003). Antibacterial and antifungal properties of essential oils. Curr. Med. Chem. 10: 813-829.
  18. Kim H., Park S., and Park H., (2008). Inactivation of Escherichia coli O157:H7 by cinnamic aldehyde purified from Cinnamomum cassia shoot. Food Microbiology 21: 105-110.
  19. Matasyoh G.L., Matasyoh J.C., Wachira F.N., Kinyua M.G., ThairuMuigai W.A., Mukiama T.K., (2007). Chemical composition and antimicrobial activity of the essential oil of Ocimumgratissimum L. growing in Eastern Kenya. Afr. J. Biotechnol., 6(6) : 760-765.
  20. Mith, Dur? R., Delcenserie V. ,Zhiri A., Daube G. , et Clinquart A?., (2014). Activit?s antimicrobiennes des huiles essentielles commerciales et de leurs composants contre les agents pathog?nes d\'origine alimentaire et les bact?ries responsables de la d?t?rioration des aliments. NourritureSciNutr . 2014 juil; 2 (4): 403-416. doi: 10.1002 / fsn3.116
  21. (1999).?Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals. Approved standard M31-A.?Wayne, Pa: NCCLS; 1999.
  22. Oussalah M., Caillet S., Saucier L., Lacroix M., (2006). Inhibitory effects of selected plant essential oils on the growth of four pathogenic bacteria: E. coli O157:H7, Salmonella Typhimurium, Staphylococcus aureus and Listeria monocytogenes. Food Control 18(5), 414-420.
  23. Pe?alver P., Huerta B., Borge C., Astorga R., Romero R., et Perea A., (2005). Antimicrobial activity of five essential oils against origin strains of the Enterobacteriaceae family. ActaPathologica, Microbiologica, etImmunologicaScandinavica, 113, 1 Ŕ 6.
  24. Pharmacop?eEurop?enne., (2011). Version 7.1 (avril 2011).
  25. Remmal A., et , (1993). Improved method for determination of antimicrobial activity of essential oils in agar medium. J. Essent. Oil Res., 5(2),179-184.
  26. Remmal A., Bouchikhi T., Rhayour K., et al., (1993a). Improved method for the determination of antimicrobial activity of essential oils in agar medium. J Ess Oil Res 5/179-184.
  27. Rota M.C., Herrera A., Mart?nez R.M., Sotomayor J.A., et Jord?n M.J., (2008). Antimicrobial activity and chemical composition of Thymus vulgaris, Thymus zygis and Thymus hyemalis essential oils. Food Control, 19, 681 Ŕ 687.
  28. Senhaji O., Faid M., Kalalou I., (2007) Inactivation of E. coli 0157:H7 by essential oil from Cinnamomumzeylanicum. Brazilian Journal of infection disease, volume 11, issue 2, p. 234-236
  29. Smith-Palmer A., Stewart J., Fyfe L., (1998). Antimicrobial properties of plant essential oils and essences against five important food-borne pathogens. Letters in Applied Microbiology 26, 118-12.
  30. Sokmen A., Gulluce M., Akpulat H. A., Daferera D., Tepe B., Polissiou M., Sokmen M. et Sahin F., (2004). The in vitro antimicrobial and antioxidant activities of the essential oils and methanol extracts of endemic Thymus spathulifolius. Food Control, 15, 627 Ŕ 634.
  31. Ultee ,? Bennik? M.H.J.,? Moezelaar R.,? (2002).? The? phenolic? hydroxyl group? of? carvacrol? is? essential? for? action? against? the? foodborne pathogen Bacillus cereus. Appl Environ Microbiol 68(4): 1561?8.
  32. Valero M., Salmeron M. C., (2003). Antibacterial activity of 11 essential oils against Bacillus cereus in tyndallized carrot broth. Int. J. Food Microbiol. 85, 73-8.
  33. Yamazaki, K., Yamamoto, T., Kawai, Y., Inoue, N., (2004). Enhancement of antilisterial activity of essential oils constituents by nisin and diglycerol fatty acid ester. Food Microbiology 21, 283-289
  34. Yashphe J., Segal R., Breuer A., and Erdreich-Naftali G., (1979).Antibacterial activity of Artemisia herbaalba. J. of Pharma. Sci.. 68(7), 924-925.

[Khribch Jamila, Smara Naima, Laghrib Farida, Zrira saadia, Nassik saadia, EL Houadfi Mohammed and Oukessou Mohamed. (2018); COMPARATIVE STUDY OF THE ANTIBACTERIAL ACTIVITY OF CINNAMON AND ORIGAN ESSENTIAL OILS AND THEIR PRIMARYCOMPONENTS ON AVIAN Escherichia coli STRAINS. Int. J. of Adv. Res. 6 (Sep). 373-381] (ISSN 2320-5407). www.journalijar.com

Khribch Jamila
Department of Biological and Pharmaceutical Sciences, Hassan II Agronomic and Veterinary Institute, BP 6202, 1010, Rabat, Morocco

DOI:

Article DOI: 10.21474/IJAR01/7688      
DOI URL: https://dx.doi.org/10.21474/IJAR01/7688

Download Full Paper

Download PDF No. of Downloads: 24 | No. of Views: 84