28Aug 2018

ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITIES OF BIOACTIVE EXTRACTS OF A POLYPORE MUSHROOM, FOMITOPSIS PINICOLA (SW.:FR.) P. KARST, FROM KASHMIR.

  • Amala Cancer Research Centre, Amala Nagar PO, Thrissur, Kerala, India. Pin ? 680555.
  • Amala Institute of Medical Sciences, Amala Nagar PO , Thrissur, Kerala, India. Pin ? 680555.
  • Department of Botany and Department of Biotechnology, University of Kashmir, Srinagar, Jammu and Kashmir, India. Pin ? 190006.
Crossref Cited-by Linking logo

  • Abstract
  • Keywords
  • References
  • Cite This Article as
  • Corresponding Author

Fomitopsis pinicola, a polypore mushroom, was investigated for its medicinal properties. In this study, antioxidant and anti-inflammatory activities of the mushroom were examined. The fruiting bodies of the mushroom were defatted with petroleum ether, and then extracted with ethyl acetate (EA) and ethanol (ETH). Both the extracts were found to possess radical scavenging activities against DPPH., ABTS.+, nitric oxide and OH. radicals. The extracts also reduced Fe3+ ions and inhibited lipid peroxidation. The extracts were further evaluated for their anti-inflammatory activity against carrageenan and formalin induced acute and chronic edema respectively in mouse model. An oral dose of 500 mg/Kg body weight of EA extract inhibited both acute and chronic inflammation by 52.77 and 46.44% and the ETH extract at the same dose inhibited 33.33 and 30.44% respectively. EA extract also inhibited carrageenan induced leukocyte infiltration in to the peritoneal cavity of mice. Both EA and ETH extracts lowered ethanol induced liver lipid peroxidation in mice nearly by 60%. Anti-inflammatory activity against croton oil induced skin inflammation showed that topical application of 20 mg of EA reduced skin inflammation and leukocyte infiltration. The extract also lowered lipid peroxidation resulted from croton oil application by almost 60%. In Cylooxygenase (COX) activity assay, EA extract inhibited the activity by 55.85%. Major chemical constituents of the mushroom are steroids, alkaloids, coumarins, phenols, tannins, saponins and carbohydrates. The study thus demonstrated that EA and ETH extracts of F. pinicola possessed significant antioxidant and anti-inflammatory activities.

  1. Ajith, T. A.,? Janardhanan, K. K. (2007): Indian medicinal mushrooms as a source of antioxidant and antitumor agents. J Clin Biochem Nutr., 40: 157-162.
  2. Aquino, R., Morelli, S., Lauro, M. R., Abdo, S., Saija, A., Tomaino, A. (2001): Phenolic constituents and antioxidant activity of an extract of Anthurium versicolor J. Nat. Prod., 64: 1019-1023.
  3. Bakshi Hamid, Smitha Sam, Tajamul Islam (2009). The free radical scavenging and the lipid peroxidation inhibition of crocin isolated from kashmiri saffron (Crocus sativus) occurring in northern part of india. Inter. J. PharmTech Res., 1: 1317-1321.
  4. Benzie, I.F.F., Strain, J.J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power,? the FRAP assay. Anal. Biochem., 239: 70-76.
  5. Damas, J., Li?geois, J.F. (1999). The inflammatory reaction induced by formalin in the rat paw. Naunyn Schmiedebergs Arch Pharmacol., 359: 220-227.
  6. Daniel, M. (1991). Methods in plant chemistry and economic botany, Kalyani publishers, New Delhi, India.
  7. Elizabeth,?K.,?Rao,?M.W.A. (1990). Oxygen?radical?scavenging ?activity?of curcumin.?Inter.?J. Pharmaceu., 58: 237‐240.
  8. Gierse Walker, M.C., Gierse, J. K. (2010). In vitro assays for cyclooxygenase activity and inhibitor characterization. Methods. Biol., 644: 131-44.
  9. Hobbs, C. (1986). Fomitopsis pinicola Red-belted polypore, in: Miovic, M. (Ed.), Medicinal Mushrooms: An Exploration of Tradition, Healing, and Culture. Book publishing company. Canada.
  10. Cheng, J., Cha-YuiLin, Huu-ShengLur, Hsuan-PeiChen, Mei-Kuang Lu (2008). Properties and biological functions of polysaccharides and ethanolic extracts isolated from medicinal fungus, Fomitopsis pinicola. Biochem., 43: 829-834.
  11. Kataoka, T., Teraoka, J., Sakoda, A., Nishiyama, Y., Yamato, K., Monden, M., Ishimori, Y., Nomura, T., Taguchi, T., Yamaoka, K. (2012). Protective effects of radon inhalation on carrageenan-induced inflammatory paw edema in mice. , 35: 713-22.
  12. Langseth, L. (1995). Oxidants, antioxidants, and disease prevention, in: Bracco, U., Jardine, N.J. (Eds), ILSI Europe concise monograph series. International life institute, Brussels.
  13. Md Al Amin, Ishtiaque A Chowdhury, Mahbub, K. M.M., Mafruhi Sattar, Masum Shahriar, Md Ruhul Kuddus, Mohammad A Rashid (2012). Anti-inflammatory and analgesic activities of asteracantha longifolia Bangla. Pharmaceu. J., 15: 171-176.
  14. Niels Heemskerk, Jos van Rijssel, Jaap D van Buul (2014). Rho-GTPase signaling in leukocyte extravasation: An endothelial point of view. Cell Adh. Migr., 8: 67?75.
  15. Ohkawa, H., Ohishi, N., Yagi, K, Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem.,95: 351-358.
  16. Pham-Huy, L.A., ?Hua He, ?Pham-Huy, C., 2008. Free radicals, antioxidants in disease and health. J. Biomed. Sci., 4: 89?96.
  17. Pino, J. A., Mesa, J., Munoz, Y., Marti, M. P., Marbot, R. (2005). Volatile components from mango (Mangifera indica ) cultivars. J. Agricult. Food Chem., 53: 2213-2223.
  18. Tiwari, P., Bimlesh Kumar, Mandeep Kaur, Gurpreet Kaur, Harleen Kaur (2011). Phytochemical screening and extraction : A review. Inter.Pharmaceutica Sciencia., 1: 98-106
  19. Rouach, H., Fataccioli, V., Gentil, M., ?French, S. W., Morimoto, M., Nordmann, R.? (1997). Effect of chronic ethanol feeding on lipid peroxidation and protein oxidation in relation to liver pathology . Hepatology., 25:? 351-355.
  1. Stamets, P. E. (2005). Antipox Properties of Fomitopsis officinalis (Vill.: Fr.) Bond. et Singer (Agarikon) from the Pacific Northwest of North America. Int. J. Med. Mush., 7: 495-506.
  2. Sunhee Shin, Seong Soo Joo, Dongsun Park, Jeong Hee Jeon, Tae Kyun Kim, Jeong Seon Kim, Sung Kyeong Park, Bang Yeon Hwang, Yun-Bae Kim (2010). Ethanol extract of Angelica gigas inhibits croton oil-induced inflammation by suppressing the cyclooxygenase - prostaglandin pathway. J Vet Sci., 11: 43?50.

[Korattuvalappil Sankaranarayanan Ravikumar, Haridas Ramya, Thekkuttuparambil Ananthanarayanan Ajith, Kainoor K Janardhanan, Manzoor Ahmad Shah, Zafar A Reshi and Khurshid Iqbal Andrabi. (2018); ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITIES OF BIOACTIVE EXTRACTS OF A POLYPORE MUSHROOM, FOMITOPSIS PINICOLA (SW.:FR.) P. KARST, FROM KASHMIR. Int. J. of Adv. Res. 6 (Aug). 1144-1156] (ISSN 2320-5407). www.journalijar.com

Kainoor K Janardhanan
Amala Cancer Research Centre, Amala Nagar PO, Thrissur, Kerala, India

DOI:

Article DOI: 10.21474/IJAR01/7624      
DOI URL: https://dx.doi.org/10.21474/IJAR01/7624

Download Full Paper

Download PDF No. of Downloads: 104 | No. of Views: 347