IDENTIFICATION OF BIOACTIVE COMPOUNDS AND ANTIBACTERIAL ACTIVITY OF MARINE GASTROPOD CONUS ACHATINUS GMELIN, 1791

ThThe aim of the present study is to screen a molluscan gastropod, Conus achatinus to find out the possible bioactive compounds present in the whole body tissues of experimental animal. The antibacterial activity was assayed against ten bacterial pathogens by Disc Diffusion Technique at three different concentrations. The crude methanolic extract showed significant antibacterial activity with minimum inhibitory zone of 2 mm against Pseudomonas aeruginosa and maximum 18 mm against Klebsiella pneumoniae at 100mg/ml concentration. It was further fractionated into four fractions using the different solvent system. Of all the fractions tested, (F2) and) (F3) showed the potent activities. The fraction F2 showed minimum inhibition zone of 9 mm against Pseudomonas aeruginosa and Proteus vulgaris and the maximum of 14 mm against Streptococcus pyogenes and F3 showed the minimum inhibition zone of 12 mm against Klebsiella pneumoniae and 20 mm against Staphylococcus aureus at 100 mg/ml concentration. The GC-MS analysis of the whole body tissue extract of Conus achatinus revealed the presence of alkaloid, amino, nitrogen, aldehyde, alcoholic, sulphur, nitrogen and steroid compounds which might be responsible for the antibacterial activity.