22May 2018

EXAMINATION OF BIOACTIVITIES ACCORDING TO THE EXTRACTION CONDITIONS OF CIRSIUM JAPONICUM VAR. USSURIENSE.

  • National Institute of Biological Resources (NIBR), Environmental Research Complex, Incheon 22689, South Korea.
  • Gyeongsangnam-do Agricultural Research & Extension Services, Jinju, 52733, South Korea. Department of Agronomy & Medicinal Plant Resources, Gyeongnam National University of Science and Technology, Jinju, 52725, South Korea.
  • Swine Science and Technology Center, Gyeongnam National University of Science & Technology, Jinju 52725, South Korea.
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This study was carried out to obtain a basic data for application in meat products via examination of bioactive materials from Cirsium japonicum var. Ussuriense tuber. Hot water extract showed high activities against BC (Bacillus cereus) and ST (Salmonella Typhimurium) as well as inhibited nitric oxide (NO) production. The supernatant of methanol fractionation (MS)50 and MS70 exhibited antibacterial activity against BC, whereas the precipitated pellet of methanol fractionation (MP)30 and MS50 showed the activity against ST. DPPH radical scavenging activity showed the highest value in MP50, but exhibited higher value in the supernatant than in the precipitated pellet. NO production in BV2 cells treated with LPS was highly inhibited in MS50 and MS70, but the activity in RAW 264.7 cells showed a high inhibition rate in MS70. As a result of ethyl acetate partition, antimicrobial activity showed high values in organic and water phases of ethyl acetate partition (MS70EAO and MS70EAW) against BC, but presented high activity in MS70EAW against ST. MS70EAO showed the highest NO production inhibition rate in BV2 and RAW 264.7 cells. In purification of Sep-Pak R18, the NO activity was showed by higher values by MS70EAO.FT (flow through), MS70EAW.FT, and MP70EAW.FT in BV2 cells, whereas observed by broad fractions in RAW264.7 cells. In summary, since the partially purified bioactive materials from C. japonicum have variously biological activities, we suggest that it is possibility of application to functional meat products.

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[Woo Young Bang, Chi Won Noh, Seung-Ho Jeon, Yeon Jo Ha, Jeong Mi Kang, Chae Won Lee, Il-Suk Kim, Tae Wan Kim and Sam Woong Kim. (2018); EXAMINATION OF BIOACTIVITIES ACCORDING TO THE EXTRACTION CONDITIONS OF CIRSIUM JAPONICUM VAR. USSURIENSE. Int. J. of Adv. Res. 6 (May). 775-783] (ISSN 2320-5407). www.journalijar.com

Sam Woong KIm
Gyeongnam National University of Science and Technology

DOI:

Article DOI: 10.21474/IJAR01/7095      
DOI URL: https://dx.doi.org/10.21474/IJAR01/7095

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