STUDIES ON BIOCHEMICAL CHANGES DURING TROPICAL TASAR SILKWORM-PEBRINE INTERACTION.
- Central Sericultural Research and Training Institute, Central Silk Board, Srirampura, Mysore-570008, Karnataka, INDIA.
- Central Tasar Research and Training Institute, Central Silk Board, Piska Nagri, Ranchi-835303, Jharkhand, INDIA.
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Tropical Tasar Silkworm, Antheraea mylitta D is infected by different pathogens. Among the different pathogens infecting tasar silkworm, pebrine disease caused by protozoa, Nosema mylitta is most destructive due to its transovarial nature. In the present study, the variation in the protein, peroxidase, catalase, acid phosphatase, amylase and endogenous hydrogen peroxide concentration during early stages of pebrine infection to tasar silkworm was analyzed. The results revealed the up-regulation in protein concentration, peroxidase, acid phosphatase, amylase enzymes activities and endogenous hydrogen peroxide concentration where as down-regulation in catalase activity. Based on the results of the present study, the changes in the reactive oxygen species and antioxidant enzymes can be used as markers to analyze the health status of the tropical tasar silkworm infected with pebrine disease.
- Ali, S. and Sharma, K.B. (2014): Impact of Diseases on the Biochemical Contents of Tropical Tasar Silkworm Antheraea mylitta International Journal of Innovative Research and Development. 3(2): 339-343.
- Baker, J.E. (1991): Purification and partial characterization of α- amylase allozymes from the lesser grain borer Rhyzopertha dominica. Insect Biochem. 21: 303-311.
- Bhawane, G.P., Chougale, A.K., Patil, S.J., Gaikwad, Y.B. and Chavan, J.A. (2013): Effects of ethanolic plant extracts on BmNPV inoculated silkworm, Bombyx mori in relation with midgut antioxidant enzymes, acid phosphatase and non-specific esterase. Sericologia 53(3&4): 182-192.
- Bradford, M.M. (1976):A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem????????????????? 72: 248-254.
- Frew,E.,?Jones,?P. and Scholes,?G.?(1983):?Spectrophotometric determination of hydrogen peroxide and organic hydropheroxides at low concentrations in aqueous solution.?Analyt Chimica Acta 155: 139?150.
- Gururaj, C.S., Sekharappa, B.M. and Sarangi, S.K. (1999): Effect of BmNPV infection on the digestive enzyme activity in the silkworm, Bombyx mori Indian Journal of Sericulture 38(2): 102?116.
- Hammerschmidt, R., Nuckles, E.M. and Kuc, J. (1982): Association of enhanced peroxidase activity with induced systemic resistance of cucumber to?Colletotrichum lagenarium.?Physiol Plant Pathol.? 20: 73?82.
- Havir,A. and Mettale,?N.A.?(1987):?Biochemical and developmental characterization of multiple form of catalase in tobacco leaves.?Plant Physiology 84:?450?455.
- Jena, K., Pandey, J.P., Priya, A., Kundu, P., Sinha, A.K., Yadav, H. and Sahay, A. (2016): Generation of cytotoxic molecules and oxidative stress in haemolymph of pebrinised tasar silkworm Antheraea mylitta? Journal of Environmental Biology. 37: 43-48.
- Madhusudhan, K. N., Nungshi-Devi, C., Lokesh, G., Singh, G. P., Sinha, A. K., Kumar, K. K., and Prasad, B. C. (2011): Impact of?Nosema mylitta(pebrine) infection on the larval parameters protein concentration and total hemocyte level in daba ecorace of?Antheraea mylitta (tropical tasar silkworm).?Microbiol. J.???? 1: 97-104.
- Madhusudhan, K. N., Iresh-Kumar, N. D. C., Singh, G. P., Sinha, A. K., Kirankumar, K. P., & Prasad, B. C. (2012): Impact of pebrine infection on catalase activity in tropical tasar silkworm (Antheraea mylitta).?Interanatioal Journal of Science and Nature.?3(1): 212-213.
- Pandey, J.P., Mishra, P.K., Kumar, D., Singh, B.M.K. and Prasad, B.C. (2010):Effect of temperature on hemocytic immune responses of tropical tasar silkworm,?Antheraea mylitta? Research Journal of Immunology 3: 169-177.
- Pech, I.I., Trudeau, D. and Strand, M. (1995): Effects of basement membrane on the behaviour of haemocytes fromPseudoplasma indudens (Lepidoptera: Noctuidae). Development of an?in vitro?encapsulation assay. Journal of Insect Physiol. 41: 801-807.
- Sahay, D.N., Roy, D.K. and Sahay, A. (2000): Diseases of Tropical Tasar Silkworm Antheraea mylitta, Symptoms and control Measures In: Lessons on Tropical Tasar. Thangavelu, K. (Ed.), Central Tasar research and training institute, Piska-Nagri, Ranchi, 104.
- Singh, G.P., Sinha, A.K., Roy, D.K., Sahay, A., Madhusudhan, K.N., Kumar, K.P. and Prasad, B.C. (2011): Cellular and Biochemical Changes of?Antheraea mylitta? on Immunization with Attenuated?Antheraea mylitta?Cytoplasmic Polyhedrosis Virus.?International Journal of Zoological Research.7: 263-271.
- Singh, G.P., Zeya, S.B., Srivastava, A.K., Prakash, B., Ojha, N.G. and Suryanarayana, N. (2008): Susceptibility of three Eco-races of tropical tasar silkworm to Antheraea mylitta cytoplasmic polyhedrosis virus (AmCPV). Caspian J. Env. Sci. 6, 161-165.
- Staykova, T.A., Ivanova, E.N., Tzenov, P.I., Vasileva, Y.B., Arkova, D.B., Pantaleeva and Petkov, Z.M. (2010): Acid Phosphatase as a Marker for Differentiation of Silkworm (Bombyx mori) strains. Biotechnology & Biotechnological Equipment. 24(1): 371-374.
[Madhusudhan. K. N., Manila2, Moorthy. S. ., Gupta. V. P., Sinha. A. K. and Sivaprasad. V. (2018); STUDIES ON BIOCHEMICAL CHANGES DURING TROPICAL TASAR SILKWORM-PEBRINE INTERACTION. Int. J. of Adv. Res. 6 (Feb). 635-647] (ISSN 2320-5407). www.journalijar.com
Central Sericultural Research and Training Institute, Central Silk Board, Srirampura, Mysore-570008, Karnataka, INDIA.