25Oct 2017

DETERMINATION OF PESTICIDES IN VEGETABLES AND FRUIT FROM SUPERMARKETS OF LUCKNOW CITY USING GAS CHROMATOGRAPHY-MASS SPECTROSCOPY.

  • Pesticide Toxicology Laboratory, Regulatory Toxicology Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), MG Marg, Lucknow, Uttar Pradesh 226001, India.
  • Department of Chemistry, Babu Banarasi Das University, Faizabad Road, Lucknow Uttar Pradesh 226028, India.
  • Analytical Chemistry Laboratory, Regulatory Toxicology Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), MG Marg, Lucknow, Uttar Pradesh 226001, India.
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Pesticide use becomes an integral part of modern agricultural practices and may cause the threat of its presence in different food commodities. In this context 31 pesticides were analyzed in different vegetables and fruit samples collected from different malls in Lucknow city, India. Extraction and cleanup of pesticide residues was done by QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method using ethyl acetate as a extraction solvent and different sorbents like primary and secondary amine, charcoal for dispersive solid phase cleanup of matrix component. Analysis of pesticide residues was done by using gas chromatography equipped with electron capture detector (GC-ECD) and detected pesticide was further confirmed by gas chromatography and mass spectrometry (GC-MS). Validated method was found accurate (% recovery 79-104%) and precise with relative standard deviation (%RSD) of less than 15. Limit of detection ranged from 1.49-5.25 mg kg-1 and limit of quantification ranged were 4.46-15.74 mg kg-1. In overall 270 analyzed samples of vegetable and fruits only 35 samples were detected with pesticide residues in which only 9 pesticides are found above MRL value according to CODEX/FSSAI MRL values. Pesticide residues were detected in cauliflower, cabbage, brinjal, green pea, broccoli, apple, grapes and banana.

  1. Kne?ević Z, Serdar M: Screening of fresh fruit and vegetables for pesticide residues on Croatian market. Food Control 2009, 20(4):419-422.
  2. Frenich AG, Vidal JL, Lopez TL, Aguado SC, Salvador IM: Monitoring multi-class pesticide residues in fresh fruits and vegetables by liquid chromatography with tandem mass spectrometry. Journal of chromatography A 2004, 1048(2):199-206.
  3. Li W, Tai L, Liu J, Gai Z, Ding G: Monitoring of pesticide residues levels in fresh vegetable form Heibei Province, North China. Environmental Monitoring and Assessment 2014, 186(10):6341-6349.
  4. Liess et al., 2005; MEA, 2005.
  5. Toan, V.D., Thao, V.D., Walder, J., Schmutz, H.R., and Ha, C.T. (2007) Contamination of?? selected organochlorine pesticide (OCPs) in surface soil in Hanoi, Vietnam. Bulletin of Environmental Contamination and Toxicology, 78, 195?200.
  6. Subhani, A., Liano, M., Huang, C.Y., and Xie, Z. M. (2001). Impact of some agronomics practices on paddy field soil health under varied ecological condition: Influence of soil moisture. Pedosphere,11, 3848.
  7. Srivastava, A.K., Trivedi, P., Srivastava, M.K., Lohani, M. and Srivastava,L.P. (2011) Monitoring of pesticide residues in market basket samples of vegetable from Lucknow? City, India: QuEChERS method. Environ Monitoring Assessment 176,465?472.
  8. Kapoor, U., Srivastava, M.K., Srivastava,? K., Patel, D.K.,? Garg, V. and Srivastava, L.P. (2013) Analysis of imidacloprid residues in fruits, vegetables, cereals, fruit juices, and baby foods, and daily intake Estimation in and around lucknow, India.? Environmental Toxicology and Chemistry 32, 723?727.
  9. Bajwa U, Sandhu KS: Effect of handling and processing on pesticide residues in food- a review. Journal of Food Science and Technology 2014, 51(2):201-220.
  10. Arienzo M, Cataldo D, Ferrara L: Pesticide residues in fresh-cut vegetables from integrated pest management by ultra performance liquid chromatography coupled to tandem mass spectrometry, vol. 31; 2013.
  11. Sinha S, Vasudev K, Vishnu Vardhana Rao M: Quantification of organophosphate insecticides and herbicides in vegetable samples using the ?Quick Easy Cheap Effective Rugged and Safe? (QuEChERS) method and a high-performance liquid chromatography?electrospray ionisation?mass spectrometry (LC?MS/MS) technique, vol. 132; 2012.
  12. Sivaperumal P, Anand P, Riddhi L: Rapid determination of pesticide residues in fruits and vegetables, using ultra-high-performance liquid chromatography/time-of-flight mass spectrometry. Food Chemistry 2015, 168(Supplement C):356-365.
  13. Sharma D, Nagpal A, Pakade YB, Katnoria JK: Analytical methods for estimation of organophosphorus pesticide residues in fruits and vegetables: a review. Talanta 2010, 82(4):1077-1089.
  14. Cherta L, Portoles T, Beltran J, Pitarch E, Mol JG, Hernandez F: Application of gas chromatography-(triple quadrupole) mass spectrometry with atmospheric pressure chemical ionization for the determination of multiclass pesticides in fruits and vegetables. Journal of chromatography A 2013, 1314:224-240.
  15. Sinha SN: Quantification of organophosphate insecticides and herbicides in vegetable samples using the ?Quick Easy Cheap Effective Rugged and Safe? (QuEChERS) method and a high-performance liquid chromatography?electrospray ionisation?mass spectrometry (LC?MS/MS) technique. Food chemistry 2012, v. 132(no. 3):pp. 1574-1584-2012 v.1132 no.1573.
  16. Aktar MW, Sengupta D, Chowdhury A: Impact of pesticides use in agriculture: their benefits and hazards. Interdisciplinary Toxicology 2009, 2(1):1-12.
  17. Guan et al., 2010; Hercegova, D?m?t?ro, & Matisova, 2007; Sinha, Rao, & Vasudev, 2012. U.S.Pujeri ,A.S.Pujar,K.G.Pujari,M.I.Kumbar and M.S.Yadawe Quantitative Analysis of Pesticide Residues in Vegetables ,Intern ational Journ al of Scientific & E ngineering Research, Volume 7, Issue 5, May-2016 386
  18. Lehotay SJ, Son KA, Kwon H, Koesukwiwat U, Fu W, Mastovska K, Hoh E, Leepipatpiboon N: Comparison of QuEChERS sample preparation methods for the analysis of pesticide residues in fruits and vegetables. Journal of Chromatography A 2010, 1217(16):2548-2560.
  19. Schenck FJ, Hobbs JE: Evaluation of the Quick, Easy, Cheap, Effective, Rugged, and Safe (QuEChERS) Approach to Pesticide Residue Analysis. Bulletin of Environmental Contamination and Toxicology 2004, 73(1):24-30.
  20. Pay? P, Anastassiades M, Mack D, Sigalova I, Tasdelen B, Oliva J, Barba A: Analysis of pesticide residues using the Quick Easy Cheap Effective Rugged and Safe (QuEChERS) pesticide multiresidue method in combination with gas and liquid chromatography and tandem mass spectrometric detection. Analytical and Bioanalytical Chemistry 2007, 389(6):1697-1714.
  21. Anastassiadeset al. 2002; Aysal et al. 2007
  22. Aysal, Ambrus, Lehotay, & Cannavan, 2007; Banerjee et al., 2007; Berrada et al., 2010; Mol et al., 2007,
  23. European Commission (2007) Method validation and quality control procedures for pesticides residues analysis in food and feed Document No.SANCO/2007/3131. ec.europa.eu/plant/prtection/resources/qualcontrol_en.pdf
  24. Food and Agriculture Organization/World Health Organization Food standards programme (2005). Codex Alimentarius Commission, Twenty-Seventh Session, Geneva, Switzerland.
  25. No.06/QAS/2012/import issues/ FSSAI Food safety and standard authority of india(ministory of health and family welfare) 3rd floor FDA bhawan, kotla road new delhi 110002

[Abhishek Kumar Singh, Anshuman Srivastava, Chandra Prabha Pandey and V. P sharma. (2017); DETERMINATION OF PESTICIDES IN VEGETABLES AND FRUIT FROM SUPERMARKETS OF LUCKNOW CITY USING GAS CHROMATOGRAPHY-MASS SPECTROSCOPY. Int. J. of Adv. Res. 5 (Oct). 1600-1607] (ISSN 2320-5407). www.journalijar.com

V.P. Sharma
CSIR IITR Lucknow

DOI:

Article DOI: 10.21474/IJAR01/5676      
DOI URL: http://dx.doi.org/10.21474/IJAR01/5676

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