14Oct 2017

STUDY OF THE KINETICS OF FORMATION OF BIOGENIC AMINES IN THE SARDINE AND MACKEREL DEPENDING ON THE MICROBIAL FLORA BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY.

  • Laboratory of Aquatic Ecology and Environment. Research Team "Biodiversity, Pollution and Water treatment, University Hassan II Faculty of Science Ain Chock, Casablanca, Morocco).
  • ONSSA (Regional Analysis Laboratory and Research, Casablanca - Morocco).
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The production of biogenic amines (histamine, putrescine, cadaverine and tyramine) was tested in strains of Enterobacteriaceae (3 Enterobacter Cloaceae and 2 Escherichia Coli) isolated from sardine (Sardina pilchardus), and (2 Raoultella Terrigena, 3 Citrobacter Braakii and 5Serratia Fonticola) isolated from mackerel (Scombers combrus), freshwater fish. In general, the bacterial strains have the ability to d?carboxylate more amino acids. Biogenic amines have been produced in particular in the exponential phase, with maximal accumulation which occurs between 8 and 32 h, according to the biogenic amine and the microbial species considered. Accumulation amines varied between fish species and in the same species between the bacterial strains, the concentration of putrescine varied from 0 mg / kg A2 in Escherichia coli isolated from sardine, ranging to 1038 mg / kg in Citrobacter Braakii A5 with mackerel. The maximum accumulation of cadaverine varied less than that of putrescine and varied from 0 mg / kg in Serratia Fonticola A6 isolated mackerel to 463.73 mg / kg in Raoultella Terigena A8 isolated as in mackerel during storage at 20 ? C. The results also indicate that the highest concentration of histamine in microbial strains studied is (384.92 mg / kg), which is six times more than the legal limit (50 mg / kg) suggested by the FDA (US FDA 2001), and is also much higher than the acceptable level (100 kg / mg) fixed for scombrids fish by the EU (EEC, 1991). And tyramine represents the lowest biogenic amine in treated samples, which is similar to previous results (Kim et al, 2009; Kuley, ?zogul and ?zogul, 2005).

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[Elyounoussi Charifa, Rachidi Abder razzak and Bekkali Mohammed. (2017); STUDY OF THE KINETICS OF FORMATION OF BIOGENIC AMINES IN THE SARDINE AND MACKEREL DEPENDING ON THE MICROBIAL FLORA BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY. Int. J. of Adv. Res. 5 (Oct). 739-748] (ISSN 2320-5407). www.journalijar.com

ELYOUNOUSSI Charifa
Laboratory of Aquatic Ecology and Environment. Research Team "Biodiversity, Pollution and Water Treatment, University Hassan II Faculty of Science Ain Chock, Casablanca, Morocco

DOI:

Article DOI: 10.21474/IJAR01/5582      
DOI URL: https://dx.doi.org/10.21474/IJAR01/5582

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