25Oct 2017

Production, purification and characterization of lichenin from Bacillus licheniformis.

  • Department of Industrial Microbiology, Jacob Institute of Biotechnology and Bioengineering, Sam Higginbottom University of Agriculture, Technology and Sciences.Allahabad-211007, (U.P) INDIA.
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Lichenin arebacteriocin produced by Bacillus licheniformis, with probiotic, fungicide and medical importance.Lichenin producing Bacillus licheniformis was isolated from soil sample, collected from research plots of SHUATS, Allahabad. All the gram positive rods was biochemical analyzed and advance bacterial identification Software showed 89 % similarity of isolated bacterial cultures with Bacillus licheniformis.The crude bacteriocin of Bacillus licheniformis, exhibited antagonistic activity against Staphylococcus aureus MCCB 0139 whereas no zone of inhibition ascertained against Escherichia coli MCCB 0017. Ten variables viz incubation temperature, incubation period, pH and medium components consisting sorbitol, lactose, yeast extract, peptone, NH4NO3, K2HPO4 and MgSO4, respectively was optimized from run number 1 to 76. Response surface data showed maximum bacteriocin production by run number 43 at pH 8.0, incubation temperature 50⁰C, and incubation period 12 hrs. Optimization illustrated that effect of increasing pH from 4.0 to 8.0, incubation temperature from 37.5⁰C and 50⁰C have significant effect on bacteriocin activity alongwith peptone concentration from 0.25 % to 0.75 % have significant effect on bacteriocin activity. The average value depicted in the top and bottom phase of the cube and left and right face of the cube, respectively showed sorbitol, peptone and MgSO4 have significant effect on bacteriocin activity. Crude lichenin was partially purified by 80 % ammonium sulphate precipitation, dialysis and ion exchange chromatography followed by quantification and estimation by Lowry?s method. SDS-PAGE characterized purified lichenin and revealed < 9 kDa of molecular weight.

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[SwetaSushmitaTigga, Rubina Lawrence and Ebenezer Jeyakumar. (2017); Production, purification and characterization of lichenin from Bacillus licheniformis. Int. J. of Adv. Res. 5 (Oct). 1448-1507] (ISSN 2320-5407). www.journalijar.com

SWETA SUSHMITA TIGGA
SAM HIGGINBOTTOM UNIVERSITY OF AGRICUTURE, TECHNOLOGY AND SCIENCES, ALLAHABAD, UTTAR PRADESH

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Article DOI: 10.21474/IJAR01/5665      
DOI URL: https://dx.doi.org/10.21474/IJAR01/5665

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