22Oct 2017

EFFECT OF DIFFERENT HIBISCUS SABDARIFFA EXTRACTS ON GLUTATHIONE AND ITS RELATED ENZYMES IN MAMMALIAN LUNG TISSUE AND CULTURE CELLS UNDER INDUCED OXIDATIVE STRESS.

  • Department of Molecular Biology, National Research Centre, Cairo, Egypt.
  • Department of Cytology and Histology, Faculty of Science, Cairo University.
  • Department of Tanning Materials and Leather Technology, National Research Centre, Cairo, Egypt.
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Oxidative stress refers to the excessive ROS production and the antioxidant system cannot be able to neutralize them. HPLC analysis of aqueous, 30% and 70% ethanol extracts of the polyphenol rich H. sabdariffa calyx (the highest phenolic, flavonoid, anthocyanin contents and antioxidant capacities) showed that cyanidin 3-O-glucoside chloride, chlorogenic acid and delphinidin derivatives were the major constituents. Production of H2O2 (prooxidant capacity) increased by increasing the ethanol extracts concentration and incubation time at pH 7.4. Gallic acid was the greatest in the production of H2O2. Pretreatment of rats with H. sabdariffa ethanol extract followed by tert-butyl hydroperoxide (t-BHP) injection partially blocked the effect of the t-BHP, as indicated by the oxidative stress markers. In rat lungs homogenates, glutathione, malondialdehyde levels and lactate dehydrogenase activity returned back almost to the normal level. The antioxidant enzymes catalase and glutathione S-transferase (GST) were increased, while glutathione peroxidase (GPx) and glutathione reductase (GR) were not affected. Histological studies indicated the presence of sever lung injury with t-BHP and H. sabdariffa extracts. In human lung fibroblast cells, H. sabdariffa extracts (150 ?g and 300 ?g) have almost no effect on GST activity while, resolved the inhibitory effect of H2O2 on GR and GPx activity.

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[Hamed R R, Hussein F E, Guneidy R A, Awad H and Ali E A A. (2017); EFFECT OF DIFFERENT HIBISCUS SABDARIFFA EXTRACTS ON GLUTATHIONE AND ITS RELATED ENZYMES IN MAMMALIAN LUNG TISSUE AND CULTURE CELLS UNDER INDUCED OXIDATIVE STRESS. Int. J. of Adv. Res. 5 (Oct). 1257-1281] (ISSN 2320-5407). www.journalijar.com

Guneidy R A
Department of Cytology and Histology, Faculty of Science, Cairo University

DOI:

Article DOI: 10.21474/IJAR01/5645      
DOI URL: http://dx.doi.org/10.21474/IJAR01/5645

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