29Oct 2017

GENETIC VARIABILITY OF THREE INDIGENOUS SUDANESE CATTLE BREEDS USING RAPD MOLECULAR MARKERS.

  • Faculty of Agriculture and Natural Resource/Kassala University/Sudan.
  • Ministry of Animal Resources, Fisheries and Rangland, Khartoum, Sudan.
  • Sudan University of Science and Technology, College of Graduate/Animal Production Department.
  • Department of Molecular Genetics, Central Laboratory, Ministry of Higher Education and Scientific Research, Khartoum, Sudan.
Crossref Cited-by Linking logo

  • Abstract
  • Keywords
  • References
  • Cite This Article as
  • Corresponding Author

This research was mainly to assess the genetic similarity and genetic distance ofthree indigenous Sudanese cattle breeds (Erashy, Butana and Kenana). Six arbitrary primers were used to detect DNA polymorphisms. Two hundred and twenty nine fragments were amplified. A high level of genetic variation was observed among and within population studied. Genetic distances between populations ranged from 0.59 to 0.64. Results of phylogenetic analysis indicated that Erashy cattle breed is genetically closer to Butana cattle breed than to Kenana cattle breed. The study also concluded that such high level of genetic differentiation within Sudanese cattle populations leads to the speculation that they might belong to different breeds despite their having the same name and similar morphological traits. This study recommended that genetic characterization of these populations needs to be clarified in the future by more specific molecular techniques.

  1. Ahmed, N. (2013). Livestock Future Perspective in Sudan: Imports threaten national producers and siphon foreign currency .news. Sudanvisiondaily.com.
  2. Ministry of Animal Racecourses, Fisheries and Rangeland ''MOARFR'' (2013).
  3. Payne,J.A. and Elamin, F.M. (1977). An interim report on the livestock industry in the Jonglei canal area. Khartoum, JEO, 1:1-125.
  4. Nei ,M. and Takezaki,N. (1996). Reconstruction of phylogenetic trees from microsatellite (STR) loci . Animal Genetics, 27:(Suppl.2):1-3.
  5. Cunningham, E.P, (1992). Animal genetic resources. Proc. Of the Workshop held at ILCA, Adis Ababa, Ethiopia.
  6. FAO. Food and Agriculture Organization of the United Nations.( 2000). World watch list for domestic animal diversity. 3rd Edition. FAO, Rome, Italy.).
  7. Meghan,, Machugh, D.E., Bradley,? D.G., (1994). Genetic characteristics of West African cattle . World Anim. Rev. 78: 59- 66.
  8. Lynch,, Milligan, B.G., (1994). Analysis of population genetic structure with RAPD markers. Mole. Ecol. 3: 91-99.
  9. Saeed, A. M., Ward, P. N., Light, D., Durkin, J. W and Wilson, R. T.,(1987). Characterization of Kenana cattle at Um Banein, Sudan. ILCA Research Report 16. ILCA, Addis Ababa, Ethiopia. 46 pp.
  10. Maule, J.P., (1990). The cattle of the tropics. Centre for Tropical Veterinary Medicine, University of Edinburgh, Great Britain. 225 pp.
  11. Epstein, H.E. (1971). The origin of the domestic animals of Africa. Volume I. Africana Publishing Corporation, New York, USA. 573.
  12. Meddani, M. A. E., (1996). Animal Wealth and animal production in the Sudan. Pub. University of Khartoum.
  13. Mason, I.L. and J.P. Maule, (1960). The indigenous livestock of Eastern and South Africa. Common wealth Bureau for Animal breeding and genetics. Tech Common. No. 14.? Famhamroyal,Common wealth Agricultural Bureaux,
  14. Osman, N. Mohammed., (1998). Impediments to investment and development strategy for the livestock resources in eastern states, PhD thesis. University of Gezira. Agri. College.
  15. El-hag, M.H.M, (2004). Characterization of dairy production and Erashy breed
  16. inElgash area .M.Sc. thesis. Faculty of Agric. Dept. of animal sci . Khartoum University.
  17. Ali, F.M. I.,(2013).Phenotypic and Genotypic Characterization of Erashy Cattle Type in El- Gash Area (Kassala State ? Sudan) PhD thesis Sudan University of Sciences and Technology. College of Graduate Studies.
  18. Maniatis, T., Fritsch, E. F., and Sambrook, J. (1989).Molecular cloning: A laboratory manual .Cold spring Harbor laboratory .Cold spring Harbor NY, volume 2.
  19. Salah Eldein, S.A., (2005). Estimation of genetic diversity in some Sudanese (Autochthonous) cattle using DNA markers. PhD thesis. Sudan University of Science and Technology College of A graduate Studies.
  20. Nei, M, and W. H. Li., (1979). Mathematical model for studying genetic variation in terms of restriction endo nucleases.? Proceedings of the National Academy of Sciences (United States of America) 76: 5269 ? 5273.
  21. William, H.E; Edelsbrunner, Herbert (1984): Efficient algorithms for agglomerative hierarchical clustering methods. Journal of classification 1(1): 7-24. Doi: 10.1007/BF01890115. ISSN 0176-4268.
  22. Elmaci,, Y. Oner, S. Ozis, and E. Tuncel, (2007). RAPD analysis of DNA polymorphism in Turkish sheep breeds. Biochem. Genet. Oct; 45(9- 10):691-6. Epub, Sep 19.
  23. Mahfouz, R., Othman, E. Othman., Soheir, M. El Nahas., and Mohamed A.A. El Barody. (2008). Genetic variation between some Egyptian sheep breeds using RAPD-PCR Research. Journal of Cell and Molecular Biology, 2(2): 46- 52.
  24. Khalidi,, Rekik, B., Haddad, B., Zourgui, L., andSouid, S. (2009). Genetic characterization of three ovine breeds in Tunisia using randomly amplified polymorphic DNA markers. Livestock Research for rural Development 22(3).
  25. DevrimK., Kaya N.(2004) : Genetic Polymorphism and Microsatellites. Kafkas Univ. Vet. Med. J. 10: 215-220.
  26. Apostol, B.L., Black, W.C., and Reiter, P. Miller, B.R. (1996).Population genetics with RAPD-PCR markers: the breeding structure of Aedesaegypti in Puerto Rico. Heredity, 76: 325-334.
  27. Hassen, F., Bekele, E., Ayalew, W., andDessie, T.? (2007). Genetic variability of five Indigenous Ethiopian cattle breeds using RAPD markers. African Journal of Biotechnology 6: 2274-2279.

[Fatima. Ali, Nawal. N. Omer, Makkawi A. Aand Ahmed S. S and Nahid. Gornas. (2017); GENETIC VARIABILITY OF THREE INDIGENOUS SUDANESE CATTLE BREEDS USING RAPD MOLECULAR MARKERS. Int. J. of Adv. Res. 5 (Oct). 1682-1690] (ISSN 2320-5407). www.journalijar.com

nahid gornas
Assistant Professor, central laboratory, ministry of higher education and scientific research

DOI:

Article DOI: 10.21474/IJAR01/5690      
DOI URL: https://dx.doi.org/10.21474/IJAR01/5690

Download Full Paper

Download PDF No. of Downloads: 21 | No. of Views: 104