22Oct 2017

IN VITRO SYNTHESIS OF LIGNIN-RESILIN BIOCOMPOSITES.

  • The Robert H. Smith Faculty of Agriculture, Food and Environment, the Hebrew University of Jerusalem, Rehovot 7610001, Israel.
  • Futuragene Ltd, Rehovot, Israel.
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Lignin is one of the most abundant biopolymers in nature but is currently underused. Extensive research is to improve the market value of lignin and its utilization. This study presents an approach to alter the mechanical properties of lignin through its combination with recombinant resilin, a rubber-like protein. Different mass ratios of resilin were added to coniferyl alcohol, together with horseradish peroxidase and hydrogen peroxide, to generate a crosslinked lignin-like?resilin biocomposite, where the lignin-like component, the so-called dehydrogenation polymer, is the product of coniferyl alcohol crosslinking. Fourier transform infrared spectroscopy and western blot analysis indicated crosslinking of the resilin protein to dehydrogenation polymer. We show that resilin binding to dehydrogenation polymer leads to a reduction in the polymer particles size as well as an increase in mechanical stiffness. This study provides insight into the mechanisms of the in vivo and in vitro crosslinking of tyrosine rich structural proteins to lignin. The crosslinked lignin-protein complex may potentially impart improved properties to lignin and to the entire plant.

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[Itan Preis, Iftach Birger, Amit Rivkin, Tal Ben Shalom, Miron Abramson and Oded Shoseyov. (2017); IN VITRO SYNTHESIS OF LIGNIN-RESILIN BIOCOMPOSITES. Int. J. of Adv. Res. 5 (Oct). 1163-1171] (ISSN 2320-5407). www.journalijar.com

Itan Preis
The Robert H. Smith Faculty of Agriculture, Food and Environment, the Hebrew University of Jerusalem, Rehovot 7610001, Israel

DOI:

Article DOI: 10.21474/IJAR01/5634      
DOI URL: https://dx.doi.org/10.21474/IJAR01/5634

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