15Aug 2017

INDUCTION OF APOPTOSIS BY THE ETHYL ACETATE FRACTION OF WOODFORDIA FRUTICOSA KURZ. FLOWERS THROUGH NFκB MEDIATION IN HEPATOCELLULAR CARCINOMA.

  • *Professor, Pharmacognosy Laboratory, School of Biosciences, Mahatma Gandhi University, Kottayam (Dist), Kerala ? 686560.
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Cancer is a disease that has made mankind distraught for the development of efficient strategies in curbing the onset, spread and mortality related to the disease. As synthetic chemotherapeutics add more complexities to the efficacy in treating cancer, plant related drug development has proven to be both an efficient and pharmaceutically favoured alternative to the same. Woodfordia fruticosa Kurz is a traditionally hailed medicinal plant. Research conducted upon the flowers of the plant has attributed hepatoprotective, antioxidant, anticancer and anti-inflammatory properties to its alcoholic extracts. The objective of the present study was to fractionate the most active alcoholic extract, analyse the phytoconstituents of the various fractions and to evaluate the anticancer efficacy and apoptosis induction potential of the most active fraction on hepatocellular carcinoma cell lines. Our study revealed that the ethyl acetate fraction of the methanolic extract of Woodfordia fruticosa flowers were rich in polyphenols such as flavonoids, tannins and terpenoids. The fraction showed profound cytotoxicity upon HepG2 cell when analysed using MTT assay. Induction of apoptosis was confirmed using Acridine Orange/Ethidium Bromide (AO/EtBr) double staining. The fraction efficiently downregulated the expression of Nuclear Factor Kappa B (NF-κB) and upregulated the expression of tumor suppressor gene p53 and cell cycle regulatory gene p21 when compared to the expression of Glyceraldehyde -3- Phosphate dehydrogenase (GAPDH) gene taken as control. Our study proved that the ethyl acetate fraction of the plant could be an active source of polyphenolics compounds with potent anticancer activity.

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[N. R Raji, P Roshni and M. S Latha. (2017); INDUCTION OF APOPTOSIS BY THE ETHYL ACETATE FRACTION OF WOODFORDIA FRUTICOSA KURZ. FLOWERS THROUGH NFκB MEDIATION IN HEPATOCELLULAR CARCINOMA. Int. J. of Adv. Res. 5 (Aug). 885-893] (ISSN 2320-5407). www.journalijar.com

Dr M. S Latha
Professor, School of Biosciences, Mahatma Gandhi University, Kottayam

DOI:

Article DOI: 10.21474/IJAR01/5132      
DOI URL: https://dx.doi.org/10.21474/IJAR01/5132

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