20Jul 2017

SCREENING OF ROOT NODULE BACTERIA FROM WILD LEGUMES FOR THEIR POSSIBLE ROLE IN PLANT GROWTH PROMOTION.

  • Department of Biotechnology,Career college, Bhopal, India.
  • Department of Biochemistry,Career college, Bhopal, India.
Crossref Cited-by Linking logo
  • Abstract
  • Keywords
  • References
  • Cite This Article as
  • Corresponding Author

In the present study, root nodule bacteria (RNB) were studied for their plant growth promoting (PGP) properties. For recovery of RNB wild leguminous plants viz., Abrus precatorious (A. precatorious), Crotolaria retusa (C. retusa), Crotolaria turnesia (C. turnesia), Desmodium sp. Gliricidia sp and Mimosa pudica (M. pudica) were collected from different areas of Bhopal. A total of 31 isolates (7 from A. precatorious, 3 from C. retusa, 4 from C. turnesia, 15 from Desmodium sp., 1 from Gliricidia sp. and 1 from M. pudica) were recovered and were studied for PGP activities viz., siderophore production, phosphate solubilization (P.solubilization), Indole acetic acid (IAA) production and ACC deaminase activity. 45.1% of the recovered isolates were positive for siderophore production with isolate DM1e showing maximum production i.e. 72.6 ?g ml-1. About 22.5% of the isolates produced IAA with isolate DM1f showing the maximum production of 10 ?g ml-1. P. solubilization and ACC deaminase activity was recorded in 54.8 and 87% isolates respectively. Further study on nitrogen fixation is underway. RNB such as rhizobia are known to promote plant growth by fixing nitrogen within root nodules and provide it to the plant for nutrition. Multifaceted beneficial effects can be obtained on plant growth and health by such RNB if they possess additional PGP properties as worked out in this study. Moreover, benefits can be obtained even if they fail to nodulate the plant (a constraint for rhizobia) but colonize the rhizosphere and compete with indigenous microbial population. Such microbes with multiple PGP properties could be developed as bioinoculants and have the potential to fetch the profit in growing biotechnology sector of India.

  1. Akhtar, S. and Ali, B (2011). Evaluation of rhizobacteria as non- rhizobial inoculants for mung beans. Aus J of crop sci.13:1723-1729.
  2. Ali, B., Sabri, A.N., Ljung, K and Hasnain, S (2009). Auxin production by plant associated bacteria:impact on endogenous IAA content and growth of Triticum aestivum Letter Appl. Microbiol. 48:542-547.
  3. Deshwal, V.K., Dubey, R.C and Maheswari, D.K (2003).Isolation of plant growth promoting Bradyrhizobium (Arachis) with biocontrol potential against Macrophomina phaseolina causing charcoal rot of peanut. Curr sci. 84:443-448.
  4. Dworken, M and Foster, J (1958). Experiments with some microorganisms which utilize ethane and hydrogen. Bacteriol.75: 592-601.
  5. Glick, B.R and Pasternak, J.J (2003). Plant growth promoting bacteria , In: Glick, B.R., Pasternak, J.J.(eds) Molecular biotechnology principles and applications of recombinant DNA , 3rd Edi, ASM Press, Washington,? 436-454.
  6. Goldstein, A.H and Krishnaraj, P.U (2007). Phosphate solubilizing microorganisms vs. phosphate mobilizing microorganisms: what separates a phenotype from a trait? In: Velazquec E, Rodriguez- Barrueco C (eds) First international meeting on microbial phosphate solubilization . Springer, Dordre,? pp-203-213.
  7. Grichko, V.P and Glick, B.R (2001). Amelioration of flooding stress by ACC deaminase-containing plant growth promoting bacteria. Plant Physiol. Biochem. 39:11-17.
  8. Hayat, R., Ali, S., Amara, U., Khalid, R and Ahamad, I (2010). Soil beneficial bacteria and their role in plant growth promotion. Annals of Microbiol. 10:1-18.
  9. Huang, H.C and Hoes, J.A.C (2008). Penetration and infection of Sclerotinia sclerotiorum by Coniothyriumminiatus. Can. J. Bot. 54:506-410.
  10. Jackson, J.E. (1932).The colorimetric determination of phosphorus. Biochem J. 26:292.
  11. Mandal, S.M., Mondal, K.C., Dey, S and Pati, B.R (2007). Optimization of cultural and nutritional conditions for indole-3-acetic acid (IAA) production by a Rhizobium sp. Isolated from root nodules of Vigna mungo (L.) Hepper. Res. J. Microbial. 2:239-246.
  12. Martinez-Viveros,O., Jorrquera, M.A., Crowly, D.E., Gajardo,G. and Mora, M.L (2010). Mechanism and practical considerations involved in plant growth promotion by Rhizobacteri. J. Soil sci. Plant Nutition. 3:293-319.
  13. Meyer, J.M and Abdallah, M.A (1978). The fluorescent pigment of Pseudomonas fluorescens : biosynthesis, purifuication and physiochemical properties. J. Gen. Microbiol. 107: 319-328.
  14. Mikanova, O and Kubat, J (1999). The practical use of P-solubilizing activity of Rhizobium strains, Rostl.Vyr. 45:407-409.
  15. Mullen, M.D (2005). Phosphorus in soils: biological interactions in: D. Hillel, C. Rosenzweig, Polwlson, K. scow, M. Singer, Sparks, D. (eds). Encyclopedia of soils in the Env. 3:210-215.
  16. Nadeem, S.M., Zahir, Z.A., Naveed, M., Asghar, H. N and Arshad, M (2010). Rhizobacteria capable of producing? Acc deaminase may mitigate salt stress in wheat, Soil Sci. Society of? American J. 74: 533-542.
  17. Somasegaran, P and Hoben, H.J (1985). Methods in Legume-rhizobium Technology. NifTAL project and MIRCEN, Department of Agronomy, 2nd Soil Science Hawaii Institute Tropical Agriculture Human research, Univ Hawaii Manoa.
  18. Sprent, J.I (2001). Nodulation in Legumes. Royal Botanical Gardens, Kew - Cromwell Press.
  19. Sprent, J.I (2007). Evolving ideas of legume evolution and diversity: a taxonomic perspective on the occurrence of nodulation. New Phytol. 174:11-25.
  20. Tsavkelova, E.A., Kilmova, S.Y., Cherdyntseva, T.A and Netrusov, A.I (2006). Microbial producers of plant growth stimulators and their practical use: a review. Appl. Biochem Microbiol. 42:117-126.
  21. White, P.J (2003). Ion transport. In: Encyclopaedia of applied plant sciences (eds. B. Thomas, Murphy and Murray), 625-634. Academic Press, London.

[Nidhi Gujar, Shaishavee Saini, Preeti Chandurkar, Tanuja Murab, Anjali Choudhary and Nidhi Tripathi. (2017); SCREENING OF ROOT NODULE BACTERIA FROM WILD LEGUMES FOR THEIR POSSIBLE ROLE IN PLANT GROWTH PROMOTION. Int. J. of Adv. Res. 5 (Jul). 992-998] (ISSN 2320-5407). www.journalijar.com

Nidhi Gujar
Assistant Professor

DOI:

Article DOI: 10.21474/IJAR01/4808      
DOI URL: https://dx.doi.org/10.21474/IJAR01/4808

Download Full Paper

Download PDF No. of Downloads: 23 | No. of Views: 110