21Jun 2017

BIOCHEMICAL AND MOLECULAR STUDIES ON SUBJECTS WITH SEDENTARY LIFESTYLE AND TYPE 2 DIABETES.

  • Department of Biochemistry, Jubilee Mission College and Research Institute, Thrissur - 680005, India.
  • Genetika, Centre for Advanced Genetic Studies, Pettah P O, Thiruvananthapuram - 695024, India.
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Type 2 diabetes is most common and comprises of 90% of people with diabetes around the world. The pathophysiology of type 2 diabetes is complex, but the major risk factors include sedentary lifestyle and behaviors coupled with multifactorial complex interaction between environment and genetic factors. Sedentary lifestyle increases the risk of type 2 diabetes and there is a preponderance evidence for the association of oxidative stress and DNA damage in type 2 diabetes. There is a growing scientific and public interest in connecting oxidative stress with a variety of pathological conditions in type 2 diabetes. The aim of the present study was to verify the level of oxidative stress that may be attributed to type 2 diabetes by Malondialdehyde (MDA) and to determine the resulting DNA damage by cytokinesis block micronuclei assay. An attempt is also being made to correlate the risk factor with type 2 diabetes. Various demographic, lifestyle, clinical and biochemical characteristics of the study subjects with type 2 diabetes was analyzed. The study was performed in 68 study subjects and 32 healthy control subjects. These study subjects were observed with raised level of MDA and increased frequency of micronuclei. This study indicates that there is a significant correlation with risk factors and mean CBMN frequency in type 2 diabetic patients.

  1. Acemoglu, H., Ceylan, A., Saka, G., Plalanci, Y., Erten, M. and Turgut, S. et al. (2001). Risk factors for diabetes in South Eastern Anatolia of Turkey, Int J Epidemiol;3(1):.1540-2614.
  2. Achalu, (2008). Health Services and Health Care Delivery Systems. Port-Harcourt, Pam UniquePublishing Co.Ltd.
  3. Ahmad, M., Ahmad, K.M., Khan A.S. (2003). Naturally occurring antioxidant vitamin levels in patients with type 2 diabetes. JAyub med Coll Abbotabad; 15(1).
  4. Baan, C.A., Nusselder, W.J., Barendregt, J.J., Ruwaard, D., Bonneux, L., et al. (1999). The burden of mortality of diabetes mellitus in the Netherlands. Epidemiology, 10: 184?187.
  5. Baliunas, D.O., Taylor, B.J., Irving, H., Roerecke, M., Patra, J., Mohapatra, S., Rehm, J. (2009). Alcohol as a risk factor for type 2 diabetes: A systematic review and meta-analysis. Diabetes Care;32: 2123-2132.
  6. Brannon and Feist. (2007). Health Psychology An Introduction to Behaviour and Health 6th Edition, United State of America, Thomspon Wadsworth Publisher.
  7. Ceriello, A. (2006). Oxidative stress and diabetes-associated complications. Endocr. Pract. 12 (l).60?62.
  8. Dandona, P., Thusu, K., Cook, S., et al. (1996). Oxidative damage to DNA in diabetes mellitus. Lancet;347: 444-445.
  9. Devlin, J.T. (1992). Effect of exercise on insulin sensitivity in humans. Diabetes care 1992.15. 1701.
  10. Ford, E., S. (2001). Vitamin supplement use and diabetes mellitus incidence among adults in the United States. Am J Epidemiol; 153:892-7.
  11. Halliwell, B. (2000). Lipid peroxidation, antioxidants and cardiovascular disease: how should we move forward? Cardiovasc Res; 47:410-8.
  12. Halliwell, B. (1997). Antioxidants and human disease: a general introduction, Nutr Rev; 55:1997. 44 ? 49.
  13. Hu, F. B., Manson, J. E., Stampfer, M. J. (2011). Diet, lifestyle and risk of type 2 diabetes mellitus in women. N Engl J Med; 161:1581-1586.
  14. Hur, N.W., Kim, H. C., Nam, C. M., Jee SH, Lee HC, Suh I. (2007). Smoking cessation and risk of type 2 diabetes mellitus: Korea medical insurance corporation study. Eur J Cardiovasc Prev Rehabil.14(2):244-9.
  15. Imperatore, G., Boyle, J. P., Thompson, T.J., Case, D., Dabelea, D., et al. (2012). Projections of type 1 and type 2 diabetes burden in the USA population. ITHS; 35: 2515-2520.
  16. International Diabetes Federation, (2003). Diabetes Atlas. 2nd edition. Brussels: Gan D, Ed. Belgium.
  17. Laight, D.W., Carrier, M. J. and Anggard, E. E. (2000). Antioxidants, diabetes and endothelial dysfunction. Cardiovasc Res.47:457-64.
  18. Mandal, B., Bandyopadhyay R., Chakrabarti S., Bandyopadhyay T.K. (2010). Assessment of oxidative stress in patients of diabetes mellitus with and without complications. JIACM.11:20?25.
  19. Pan, A., Wang, Y., Talaei, M., Hu, F. B., Wu, T. (2015). Relation of active, passive, and quitting smoking with incident type 2 diabetes: a systematic review and metaanalysis. The lancet Diabetes & Endocrin. 3: 958-967
  20. Petrie, J. R., Person, E. R. and Sutherland C. (2011). Implications of genome wide association studies for the understanding of type 2 diabetes pathophysiology. Biochemical Pharmacology: 2011. 81: 471-477.
  21. Pont, F., Duvillard, L., Florentin, E., Gambert, P., Verge`s, B. (2002). High-density lipoprotein apolipoprotein A-I kinetics in obese insulin resistant patients. An in vivo stable isotope study. Int. J. Obes. Relat. Metab. Disord. Rocchini, A., P. (2002). Childhood obesity and diabetes epidemic. New Engl J Med. 346:854? 855.
  22. Roglic, G., Unwin, N., Bennett, P. H., Mathers, C., Tuomilehto, J., Nag, S., Connolly, V., King, H. (2005). The burden of mortality attributable to diabetes: realistic estimates for the year 2000. Diabetes Care; 28:2130? 5.
  23. Sairenchi, T., Iso, H., Nishimura, A., Hosoda, T., Irie, F., Saito, Y., et al. (2004). Cigarette smoking and risk of type 2 diabetes mellitus among middle-aged and elderly Japanese men and women. Am J Epidemiol; 160(2):158-62.
  24. Salonen, J. T., Nyyssonen, K., Tuomainen, T. P., Maenpaa, P. H., Korpela, H. et al. (1995). Increased risk of non-insulin dependent diabetes at low plasma vitamin E concentrations: a four year follows up study in men. BMJ; 1995. 311: 1124-7.
  25. Yfjord, J., Bodvarsdottir, S. (2005). Genomic instability and cancer: networks involved in response to DNA damage. 2005. Mutat Res;592:18-28. 5.

[Viji Krishnan, Dinesh Roy D and Kavya P. (2017); BIOCHEMICAL AND MOLECULAR STUDIES ON SUBJECTS WITH SEDENTARY LIFESTYLE AND TYPE 2 DIABETES. Int. J. of Adv. Res. 5 (Jun). 1371-1376] (ISSN 2320-5407). www.journalijar.com

Dr. Dinesh Roy D
Genetika, Centre for Advanced Genetic Studies, Pettah P O, Thiruvananthapuram - 695024, India

DOI:

Article DOI: 10.21474/IJAR01/4546      
DOI URL: https://dx.doi.org/10.21474/IJAR01/4546

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