21Jun 2017

A COMPARATIVE MICROBIAL QUALITY ASSESSMENT OF FRESH SEAFOODS COLLECTED FROM WHOLESALE AND RETAIL MARKETS IN EASTERN PROVINCE OF SAUDI ARABIA.

  • Fisheries Research Center, Deputy Ministry for Fisheries, Ministry of Environment, Water & Agriculture, Kingdom of Saudi Arabia.
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A study was conducted to comparatively evaluate the microbial quality of seafoods collected from fish markets in Eastern Province of Saudi Arabia during the period from January 2015 until May 2016. In this study, Fishes, Prawn and Cuttlefish samples were analysed in 2 sampling categories such as samples collected from wholesale fish market (200 samples) and retail fish markets (200 samples). Total Plate Count (TPC) and Total Coliforms Count (TCC) in the skin attached muscles were analysed using Plate Count Agar (PCA) and Violet Red Bile Agar (VRBA), respectively. The mean Total Plate Count of bacteria and mean Total Coliform Count detected in all the raw seafood samples collected from retail markets were comparatively higher than that of seafood samples collected from wholesale markets as per the limits of SASO. From the result it can be concluded that most of the seafoods sold in the retail markets are not met the standards for human consumption. Thus, it is strongly recommended to enhance proper controlling of the storage and shelf life of the seafoods by implementing preventive measures and application of sanitation procedures in the fish markets to monitor the quality of raw seafoods by reducing the prevalence of the pathogens.

  1. Abolagba, O.J. and Melle, O.O (2008). Chemical composition and keeping qualities of a Scaly Fish Tilapia (Oreochromis niloticus) Smoked with two Energy Sources. African J. Gen. Agric., KLOBEX, 4(2): 113-117.
  2. Adebayo-Tayo A.C., Odu, N.N., Michael, M.U. and Okonko, I.O. 2012. Multi-Drug Resistant (MDR) Organisms isolated from Sea-foods in Uyo, South-Southern Nigeria. Nature and Science 10: 61-70.
  3. Allen GH, Busch RA, Morton AW. Preliminary bacteriological studies on wastewater-fertilized marine fishponds. Humboldt Bay, California Bay, California. In: Advances in Aquaculture Fishing News Books, Oxford, England 1979; pp. 492-8.
  4. Austin B. and Austin DA. (1996) Bacterial pathogens of fish. Journal of Applied Bacteriology, 58 (6): 483-506.
  5. Batz MB, Doyle MP, Morris JG Jr, Painter J, Singh R, Tauxe RV et al. Attributing illness to food. Emerging Infectious Diseases, 2005 July. Available from http://www.cdc.gov/ncidod/EID/vol11no07/04-0634.htm
  6. Chao, K.K., Chao, C.C. and Chao, W.L.2003.Suitbility of the traditional microbial indicators and their enumerating methods in the assessment of fecal pollution of subtropical freshwater environments. Journal of Microbiology Immunological Infection.36, pp 288-293.
  7. Clem, J.D. and S. Garrett, 1968. Sanitation Guidelines for the Breaded-Shrimp Industry. U.S. Dept. of Interior, Fish and Wildlife Service, Bureau of Commercial Fisheries, 308.
  8. Clucas, I.J. and Ward, A.R (1996): Post Harvest Fisheries Development. A Guide to Handling, Preservation, Processing and Quality. Chatham Maritime, Kent ME4TB, United Kingdom.pp: 665.
  9. Consuelo L. Sousa, Jos? A. Freitas, L?cia F.H. Louren?o, Eder A. F. Araujo and Maria Regina S. Peixoto Joele,2014. Microbiological contamination of surfaces in fish industry, African Journal of Microbiology Research, 8(5), pp. 425-431, 29 January, 2014
  10. Doyle, M.P. and M.C. Ericson, 2006. Clossing the door on the faecal coliform assay. Microbe., 1: 162-163.
  11. Emikpe, B.O., Adebisi, T. and Adedeji, O.B. 2011. Bacteria load on the skin and stomach of Clarias Gariepinus and Oreochromis Niloticus from Ibadan, South West Nigeria: Public health implications. Journal of Microbiology and Biotechnology Research 1: 52-59.
  12. Falana, A.A., Determination of profiles of human bacteria pathogens in Nigerian fish and Seafood for Export., 2003. Proceedings of a final Research Coordination meeting held in Mexico city, Mexico., 22-26 July 2002., IAEA-TECDOC-1431
  13. FAO: Corporate Document Repository., Quality aspects associated with Seafood.
  14. Gonzalez-Fandos, E., 2004. Evaluation of the microbiological safety and sensory quality of rainbow trout (Oncorhynchus mykiss) processed by the sous vide method. Food Microbiol., 21(2): 193-201.
  15. Herrera, F.C., J.A. Santos, A. Otero and M.L. Garcia-Lopez, 2006. Occurrence of Food borne pathogenic bacteria in retails prepackage portions of marine fish in Spain. J. Appl. Microbiol., 100(3): 527-36.
  16. In: Regenstein C.E and Regenstein M.J 1991. Introduction to fish technology. Page 64.Published by Van Nostrand Reinhold, New York.
  17. Huss, H.H., Dalgaard, D., Hansen, L., Ladefoged, H., Pedersen, A., and Zittan, L. 1974. The influence in catch handling on the storage life of iced cod and plaice. Journal of Food Technology.9, 213-221.
  18. Huss, H.H. 1997. Control of indigenous pathogenic bacterial in seafood. Food Control 8: 91-98.
  19. International Commission of Microbiological Specifications for Foods. ICMSF. 1986. Microorganisms in Foods 2- Sampling for Microbilogical analysis: Principles & Specific applications. Toronto, Ontario, Canada, University of Toronto Press (2nd Edition).
  20. Janice, M. H. and J. S. Lee.1968. Microbiological evaluation of pacific shrimp processing. Applied Microbiology, American Society for Microbiology 18: 2.
  21. Jha P, Roy RP, Barat S (2010). Application of sensory and microbial analysis to assess quality of fish in Siliguri city of West Bengal, India. J. Environ. Biol. 31:587-594.
  22. Koutsoumanis, K. and G.J. Nychas, 2000. Application of systemic experimental procedure to develop a microbial model for rapid fish shelf life predictions. Int. J. Food Microbiol, 60(2-3): 171-184.
  23. Lin Ming-hsien. ICDF Aquaculture Development in the Kingdom of Saudi Arabia, Saudi Arabia Technical Mission, International Cooperation & Development, pp. 21-24
  24. Lyhs, U., 2009. Microbiological Methods. In: Fishery Products Quality, Safety and Authenticity, Rehbein, H. and J. Oehlenschlager (Eds.). Chapter 15, John Wiley and Sons, Inc, New Jersey, ISBN-13: 9781444322675, pp: 318-348
  25. Mahumda, B., Abu, T.A.A., D.M. and P.S. 2010. A comparative microbiological assessment of five types of selected fishes collected from two different markets. Biol. Res., 4(5): 259-165.
  26. Nissen, H.; Maugesten, T. and Lea, P. (2001):Survival and growth of Escherichia coli 0157:H7, Yersinia enterocolitica and Salmonella enteritis on decontaminated and untreated meat. Meat Science; 57:291-298.
  27. Olayemi AB, Adebayo O, Ojo AO. Microbial flora of six freshwater fish species from Asa River, Ilorin, Nigeria.Rev Biol Trop 1991; 39: 165-7.
  28. Onyango, M.D., Sarah Wandili, Rose Kakai, Eliud, N.W., 2009. Isolation of Salmonella and Shigella from fish harvested from the Winam Gulf of Lake Victoria, Kenya., J. Infect. Developing Countries., 3 (2): 99-104
  29. Pal, M. 2010. Fish hygiene. MSc Lecture Notes. Addis Ababa University, Faculty of Veterinary Medicine, Debre Zeit, Ethiopia. Pp.1-11.
  30. Pal, M. 2012. Food spoilage. Ph.D. Lecture Notes. Addis Ababa University, College of Veterinary Medicine, Debre Zeit, Ethiopia. Pp.1-8.
  31. Priyanka Rani Majumdar, Bhakta Supratim Sarker, Swapan Chandra Dey, Debashish Saha, Ripon Kumar Adhikary and Shuvagato Mondal., 2014. Bacterial Hazards Especially Pathogenic to Human in Consumable Marine fishes of Noakhali Sadar, Bangladesh., American Journal of Food Technology
  32. Reza, M. S., et al. Shelf life of several marine fish species of Bangladesh during ice storage. Int. J. Food Science and Technology 44: 1485?1494.
  33. Rhea, F. 2009. Microbiology handbook: Fish and seafood. Leather head Food International Ltd. Surrey, UK.
  34. Rombouts FM, Nout R. Food Microbiology and Hygiene. Encyclopedia of Human Biology Academic Press, 1994; III: 661-665.
  35. Salim, G. M.2005. A Study on the bacteriology of smoked thai Pangas (Pangasius hypophthylmus). Sc. Thesis, Bangladesh Agricultural University, Mymensingh, Bangladesh.
  36. SASO 1571 (GS 1361) Chilled Shrimps.
  37. SASO 708 (GS 380) Chilled Fish.
  38. SASO 1556 (GS 1016) Microbiological Criteria for Foodstuffs-Part 1
  39. Shankar Chandra Mandalm, M. Hassan, M.S Rhman, M.H Manik, Zahid H.M and M.D Sirajul Islam (2009): World Journal of fish and Marine science (3): 160-166. ISSN 1992- 0083
  40. Temelli S, Dokuzlu C, Sen MKC (2006). Determination of microbiological contamination sources during frozen snail meat processing stages. Food Control 17:22-29.
  41. Thabit Zahran Salim Al- Abdessalam (1995). Marine Species of the Sultanate of Oman, Marine Science and Fisheries Centre, Ministry of Agriculture and Fisheries, Oman.
  42. USFDA BAM (Online), http://www.fda.gov/Food/FoodScienceResearch/LaboratoryMethods/ucm2006949.htm
  43. Wajeeha S. Al-Baharna (1986). Fishes of Bahrain, Ministry of Commerce and Agriculture, Directorate of Fisheries, Bahrain.
  44. Wekell, M.M., Manger, R., Colburn, K., Adams, A and Hill, W., 1994. Microbiological Quality of Seafoods: Viruses, Bacteria and Parasites., Shahidi F and Botta, J.R., Eds., Seafoods Chemistry, Processing technology and Quality, Chapman & Hall, Glasgow., pp 220-232
  45. Wogu, M.D., Maduakol, M., 2010. Evaluation of Microbial Spoilage of some aqua cultured fresh fish in Benin city, Nigeria., Ethiopian J. Environ. Studies Manag., 3 (36): 18-22
  46. Yagoub, S.O. and T.M. Ahmed, 2004. Pathogenic Microorganisms in fresh water Samples collected from Khartoum central market. Sudan J. Vet. Sci. Anim. Husbandry, 43(1-2): 32-37.
  47. Yagoub, S.O. 2009. Isolation of Enterobacteria and Pseudomonas species from raw fish sold in fish market in Khartoum State. Journal of 27.Bacteriological Research. 1(7):85-88.

[Mohamed Ibrahim, M.A., Sami Shabeeb Al Shabeeb, Nabil Bin Ibrahim Fita and Ghamri H. Al Ramadhan. (2017); A COMPARATIVE MICROBIAL QUALITY ASSESSMENT OF FRESH SEAFOODS COLLECTED FROM WHOLESALE AND RETAIL MARKETS IN EASTERN PROVINCE OF SAUDI ARABIA. Int. J. of Adv. Res. 5 (Jun). 1227-1235] (ISSN 2320-5407). www.journalijar.com

Mohamed Ibrahim
Fisheries Research Center, Deputy Ministry for Fisheries, Ministry of Environment, Water & Agriculture, Kingdom of Saudi Arabia.

DOI:

Article DOI: 10.21474/IJAR01/4528      
DOI URL: https://dx.doi.org/10.21474/IJAR01/4528

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