23May 2017

AGROBACTERIUM-MEDIATED GENETIC TRANSFORMATION AND REGENERATION FROM LEAF EXPLANTS OF SOLANUM THORVUM (SWARTZ) A MEDICINALLY IMPORTANT PLANT.

  • Department of Botany, SRRGovt.Arts & ScienceCollege Karimnagar ? 505001.
  • Department of Botany, Govt.Degree & P.G.College, Jammikunta, 505122.
  • Department of Botany, Govt. Degree& P.G.College Narsampet- 506132.
  • Department of Botany, Govt.Degree & P.G.College for Women Karimnagar- 505001.
  • Department of Botany, UniversityCollegeKakatiyaUniversityWarangal ? 506009.
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Solanum torvum commonly known as turkey berry, devil's-fig, or prickly Solanum is a plant of great economic importance. Its extract, rich in Solanine and Solasodine (steroidal alkaloids) have beneficial effect on bronchial asthma. It is cultivated in tropics for its immature edible fruits. In India the S. torvum is used in medicines to cure diabetes, asthma, cholera, bronchitis and dysuria. The fresh or dry leaf and fruit are said to reduce blood cholesterol level. In this plant is susceptible to a number of diseases and pests capable of causing serious crop losses. This problem has been addressed by hybridizing S. torvum plant with wild resistant Solanum species, which present a wild genetic diversity and are source of useful agronomic traits. S. torvum plant tissues present a high morphogenetic potential that is useful for developmental studies as well as for establishing biotechnological approaches to produce improved varieties such as embryo rescue, in vitro selection, somatic hybridization and genetic transformation, In order to generate S. torvum leaf explants were cultured on Murashige and Skoog medium containing (0.5mg/L) IAA supplemented with BAP, in addition to either (100 mg/L) Kanamycin and (200mg/L) cefotaxime after co-cultivation with disarmed Agrobacterium tumefaciens harboring a plant expression binary vector. Explants co-cultivated with A. tumefaciens LBA 4404 produced putative transgenic adventitious shoots at (2.3?0.4) shots/explants, respectively. The transgenic plants were grown to maturity after vernalization in a greenhouse and appeared morphologically normal. Progeny analysis of independent transgenic plants demonstrated that the gus gene was transmitted in a Mendelian pattern in 3 lines, indicating a single copied gene was incorporated into the genome.

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[Ugandhar T, Odelu G, Parvathi. D, Anitha Devi U and Venkateshwarlu M. (2017); AGROBACTERIUM-MEDIATED GENETIC TRANSFORMATION AND REGENERATION FROM LEAF EXPLANTS OF SOLANUM THORVUM (SWARTZ) A MEDICINALLY IMPORTANT PLANT. Int. J. of Adv. Res. 5 (May). 896-904] (ISSN 2320-5407). www.journalijar.com

Dr.Thirunahari Ugandhar
Department of Botany, SRR Govt. Arts & Science College Karimnagar – 505001

DOI:

Article DOI: 10.21474/IJAR01/4193      
DOI URL: http://dx.doi.org/10.21474/IJAR01/4193

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