29May 2017

ANALYSIS OF STEVIOL GLYCOSIDES FROM Stevia rebaudiana BY USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY AND REFRACTOMETER, HENCE THE CORRELATION BETWEEN BOTH ANALYSER.

  • Faculty of Plantation and Agrotechnology, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia.
  • Faculty of Agricultural, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
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The sweet diterpene glycosides extracted from the leaves of Stevia rebaudiana (Bert) Bertoni are of increasing interest in recent years as possible sugar substitutes. The approach for this study was towards farmers and household which more applicable for them to use portable refractometer rather than analysis in laboratory using HPLC. Other than that, this finding could be a help in reducing cost due to high costing of HPLC. Our objectives were to investigate the application of refractometer in analysis of steviol glycosides and its correlation with HPLC. The calibration curve for standard stevioside and Reb A shows the value of R2 , 0.9903 and 0.9901 respectively. The results obtained from the MINITAB software version 16, shows the Pearson coefficient, r was 0.986 and the P-value shows the significant value which was 0.000 (p<0.05). Besides, the scatter plot shows the linear correlation for both analyser.

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[A.H. Nur Sabreena, Y. Nor Azma, A. Asmah and O. Mohamad. (2017); ANALYSIS OF STEVIOL GLYCOSIDES FROM Stevia rebaudiana BY USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY AND REFRACTOMETER, HENCE THE CORRELATION BETWEEN BOTH ANALYSER. Int. J. of Adv. Res. 5 (May). 1559-1563] (ISSN 2320-5407). www.journalijar.com

A.H. Nur Sabreena
Faculty of Plantation and Agrotechnology, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia

DOI:

Article DOI: 10.21474/IJAR01/4283      
DOI URL: http://dx.doi.org/10.21474/IJAR01/4283

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