25Apr 2017

GREEN SYNTHESIS OF SILVER NANOPARTICLES USING Citrus reticulata FRUIT PEEL AQUEOUS EXTRACT AND THEIR ANTIBACTERIAL ACTIVITY.

  • Department of Biotechnology, Dr. N.G.P. Arts and Science College, Coimbatore, Tamilnadu, 641048, India.
  • Department of Botany, Periyar University, Periyar Palkalai Nagar, Salem, Tamil Nadu -636011, India?
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Environmental friendly approach or green chemistry synthesis of metallic nanoparticles has become new growing branch in nanobiotehnology. In this present work a simple and environmental friendly silver nanoparticles (AgNPs) were prepared using Citrus reticulata fruit peels aqueous extract as the reducing agent guided by the principles of green chemistry. The fruit peels aqueous extract was challenged with silver nitrate solution for the production of AgNPs in room temperature. The crystalline phase and morphology of AgNPs were determined from UV-Vis spectroscopy, Fourier transform infrared (FTIR) spectra, X-ray diffraction (XRD), Scanning Electron Microscopy (SEM) and Energy Dispersive X-ray Spectroscopy (EDX). The UV-Vis spectrum indicated that the surface plasmon broad peak was observed at 466 nm after 6hr of incubation. XRD spectrum revealed that the average size of Citrus reticulata fruit peels aqueous extract mediated AgNPs obtained approximately 24 nm by using the Debye-Scherrer equation. SEM image showed uniformly distributed AgNPs on the surface of the cell with high agglomeration. EDX analysis revealed that the presence of silver which was confirmed by the Ag peak at 3.28 keV. In addition, the Citrus reticulata fruit peels aqueous extract mediated AgNPs loaded discs were tested for antibacterial properties against Streptococcus pyogenes, Staphylococcus aureus, Bacillus subtilis Escherichia coli, Salmonella paratyphi and Klebsiella pneumoniae and found that the obtained metallic AgNPs have a good antibacterial activity and can be used for biological applications.

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[ Shanmugavadivu. M, Selvam Kuppusamy. (2017); GREEN SYNTHESIS OF SILVER NANOPARTICLES USING Citrus reticulata FRUIT PEEL AQUEOUS EXTRACT AND THEIR ANTIBACTERIAL ACTIVITY. Int. J. of Adv. Res. 5 (Apr). 700-708] (ISSN 2320-5407). www.journalijar.com

Shanmugavadivu.M
Assistant Professor, Department of Biotechnology, Dr. N.G.P. Arts and Science College

DOI:

Article DOI: 10.21474/IJAR01/3866      
DOI URL: http://dx.doi.org/10.21474/IJAR01/3866

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