30Mar 2017

ISOLATION OF ENTEROBACTERIACEAE FROM RAW SEAFOODS SOLD IN FISH MARKETS IN EASTERN PROVINCE OF SAUDI ARABIA.

  • Fisheries Research Center, Deputy Ministry for Fisheries, Ministry of Environment, Water and Agriculture, Kingdom of Saudi Arabia.
  • Abstract
  • Keywords
  • References
  • Cite This Article as
  • Corresponding Author

Aim of this investigation is to provide the general information about prevalence of members of Enterobacteriaceae in raw seafoods sold in fish markets in Eastern province of Saudi Arabia. Studies were carried out to isolate and identify different bacterial species belongs to the family of Enterobacteriaceae in 448 seafood samples comprising Fishes (353), Prawns (49) and Cuttlefishes (46), collected during the period from January 2015 until May 2016. Bacterial isolates were characterized on specific culture media and further confirmed using API 20E Strips and identified by “api web”. Out of 448 samples analysed, 304 (67.85%) samples showed positive isolation of Enterobacteriaceae. Morganellamorganii showed highest prevalence (18.52%), followed by Proteussp (12.27%), Citrobactersp (8.03%), Enterobactersp (6.69%), Providenciasp (5.58%), Hafniaalvei (4.46%), Escherichia coli (4.24%), Klebsiellasp (2.90%), Rahnellaaquatilis (2%), Serratialiquefaciens (1.78%) and Pantoeaagglomerans (1.33%). The presence of these organisms in the fresh seafood samples showed that the source of contamination may be from environment, catching, unhygienic handling and transportation. Thus it is strongly recommended that the improvements in handling and processing are needed to minimize the prevalence of pathogenic bacteria by implementing preventive measures and application of appropriate procedures for hygiene practices to monitor the quality of raw seafoods.


  1. Austin, B and Austin D.A., 1985. Bacterial pathogens of Fish., Journal of Applied Bacteriology., 58: 483-506
  2. Bakr WMK, Hazzah WA, Abaza AF, 2011: Detection of Salmonella and Vibrio species in some seafood in Alexandria. J A M Sci 7: 663-668.
  3. Banwart, G., 1989. Basic Food Microbiol., 2nd Pp. 312., An. Avi. Book., Published by Van Nostrand Reinhold., Newyork.
  4. Biradar, R.S., OJHA, S.N., Ayyappan, S., and Salim, S.S., Fishing redefined, Times food processing journal., 2001. pp: 28-31
  5. Bryan, F.L., 1980. Food-borne diseases in the United States associated with meat and poultry., J. of Food Protect., 43: 140-150
  6. Cohen, J and H. I. Shuval., 1973. Coliform, Fecal coliform and Fecal Streptococci as indicators of water pollution. Water Soil Pollution, 2: 85-95
  7. Dos Santos, D.S., Solidonio, E.G., Costa, M.C.V.V., Melo, R.O.A., De souza, I.F.A.C., Silva, G.R., and Sena, K.X.F.R., 2015. Study of the Enterobacteriaceae group CESP (Citrobacter, Enterobacter, Serratia, Providencia, Morganella and Hafnia): A Review., The Battle against Microbial Pathogens: Basic Science., Technological Advances and Educational Programs (A. Mendez-Vilas, Ed)., pp. 794-805
  8. Etzel, V., Meyer, C., Ballin, U., Krause, R., 1998. Investigation for the evaluation of Quality of Nile Perch after arriving at German wholesars. FSTA, 30 (12): 226
  9. Falana, A.A., Determination of profiles of human bacteria pathogens in Nigerian fish and Seafood for Export., 2003. Proceedings of a final Research Coordination meeting held in Mexico city, Mexico., 22-26 July 2002., IAEA-TECDOC-1431
  10. FAO: Corporate Document Repository., Quality aspects associated with Seafood.
  11. FAO (Food and Agriculture Organization)., 1983. Support and Development of the Retail Trade in perishable Fishery products. pp 10-13 (Eddie, G. C. Edit), FAO fisheries technical paper No. 235 FIIU/T235
  12. Figencetinkaya, TulayElalMus, Umutcelik., 2014. Occurrence of Vibrio, Salmonella and Staphylococcus aureus in retail fresh Fish, Mussel and Shrimp., ACTA VET. BRNO., 83: 73-78
  13. Gram, L., Huss, H., H., 1996. Microbiological spoilage of fish and fish products. Int. J. Food Microbiol., 33: 121-137
  14. Grimont, P.A.D. and Grimont, F., 1978. The genus Serratia.,Annu. Rev. Microbiol., 32: 221-248
  15. Hui Liu, Junmin Zhu, Qiwen Hu, XiancaiRao, 2016. Morganellamorganii, a non-negligent opportunistic pathogen (Review)., International Journal of Infectious Diseases., 50 (2016): 10-17
  16. Huss, H.H., Ababouch, L. and Gram, L., 2004. Assessment and management of seafood safety and quality. FAO Fisheries Technical Paper., No. 444., Rome
  17. International Life Sciences Institute (ILSI) 2011. The Enterobacteriaceae and their significance to the food industry., LSI Europe Report Series., pp 49
  18. KaleyTash, 2004. Rahnellaaquatilis Bacteremia from a suspected urinary source., Journal of Clinical Microbiology., May 2005., pp 2526-2528
  19. Kumar R, Surendran PK, Thampuran W, 2009: Distribution and Genotypic characterization of Salmonella serovars isolated from tropical seafood of Cochin, India. J. Appl. Microbiol. 106: 515-524.
  20. Mai F. Elsherief., Mohamed M. Mousa., HosamAbd El-Ghalil., Engy F. El-Bahy., 2014. Enterobacteriaceae associated with Farm Fish and Retailed Ones., Alexandria Journal of Veterinary Sciences., 42: 99-104
  21. Maria Carla Liberto, Giovanni Matua, RossanaPuccio, Terera Lo Russo, Elena Colosima, Emanuel Foca, 2009. Six cases of sepsis caused by Pantoeaagglomerans in a teaching hospital., New Microbiologica., 32: 119-123
  22. , Joseph, A., Ayoola., Simeon, O., 2015. Comparative studies of bacteria load in fish species of commercial importance at the Aquaculture unit and Lagoon front of the university of Lagos., International Journal of Fisheries and Aquaculture., Vol. 7 (4)., pp 37-46
  23. Onyango, M.D., Sarah Wandili, Rose Kakai, Eliud, N.W., 2009. Isolation of Salmonella and Shigella from fish harvested from the Winam Gulf of Lake Victoria, Kenya., J. Infect. Developing Countries., 3 (2): 99-104
  24. Podschun, R., Fischer, A and Ullmann, Y., 2001. Characterisation of Hafniaalvei isolates from human clinical extra-intestinal specimens: Haemagglutinins, Serum resistance and Siderophore synthesis., J. Med. Microbiol., Vol. 50: 208-214
  25. Podschun, R., Ullmann, U., 1998. Klebsiellaspp as nosocomial pathogens: epidemiology, taxonomy, typing methods and pathogenicity factor.,Clin. Microbiol. Rev., 11: 589-603
  26. Popovic, N.T., Skukan, A.B., Dzidara, P., Coz-Rakovac, R., Strunjak-Perovic, I., Kozacinski, L., Jadan, M., Brlek-Gorski., 2010. Microbiological Quality of Fresh and Frozen Seafood caught of the Ardiatic coast of Croatia., Vet Med., 55: 233-241
  27. Priyanka Rani Majumdar, BhaktaSupratimSarker, Swapan Chandra Dey, DebashishSaha, Ripon Kumar Adhikary and ShuvagatoMondal., 2014. Bacterial Hazards Especially Pathogenic to Human in Consumable Marine fishes of NoakhaliSadar, Bangladesh., American Journal of Food Technology
  28. Rahkia, S., Schaberg, D., Johnson, S., 1998. Significance of Salmonella serotypes in fish.,Epidemiol. Infect., 125 (9): 1284-1288
  29. Renat Albuquerque Costa., 2013. Escherichia coli in seafood: A brief overview., Advances in Bioscience and Biotechnology., 4: 450-454
  30. Rodriguez, A.I., hariharan, H., Nimrod, S., 2011. Occurrence and Antimicrobial drug resistance of potential bacterial pathogens from Shellfish, including Queen Conchs (Strombusgigas) and Whelks (Cittarum pica) in Grenada., Web Med Central Microbiol., 2: WMC001943
  31. Romero Jarero Jorge, PilarNegrete Redondo and Maria del Carmen MonroyDosta, 2016. Bacterial load comparison of marine fish collected and commercially obtained for human consumption in western region of Yucatan peninsula, Mexico., International Journal of Aquatic Science., Vol. 7., No. 1., 6-12
  32. Rusul, G., MahyuddinDahan, M., Dollah, M., 1991. A survey of microbiological quality of fish sold in two local wet markets, Pertanika., 14 (3): 243-247
  33. Sanaa O. Yagoub., 2009. Isolation of Enterobacteriaceae and Pseudomonas from raw fish sold in fish market in Khartoum state., Journal of Bacteriology Research., Vol. 1 (7) pp 085-088
  34. SehnazYasemin TOSUN., Didem UCOK Alakavak.,Suhendan, MOL., 2016. Isolation of Salmonellaspp and other members of Enterobacteriaceae from horse Mackerel (Trachurustrachurus), sold in public markets of Istanbul, Turkey., Journal of Food and Health Sciences., 2 (2): 82-89 (2016)
  35. Shabarinath S, Kumar HS, Khushiramani R, Karunasagar I, 2007: Detection and Characterization of Salmonella associated with Tropical seafood. Int. J. Food Microbiol. 114: 227-233.
  36. , 2008. Electrolyzed Oxidizing water Treatment as a Post-harvest Process for controlling histamine formation in fish., A thesis submitted to Oregon State university, in partial fulfillment of the requirements for the degree of Master of Science.
  37. United Nations (2004): Global action for women towards sustainable and equitable development, Agenda 21, Chapter 24. United Nations Environment Programme.
  38. USFDA BAM (Online), http://www.fda.gov/Food/FoodScienceResearch/LaboratoryMethods/ucm2006949.htm
  39. Varnam, A.H., Evans, M.G., 1991. Food borne pathogens., Wolfe Publishing Ltd., Aylesbury., England.
  40. Wekell, M.M., Manger, R., Colburn, K., Adams, A and Hill, W., 1994. Microbiological Quality of Seafoods: Viruses, Bacteria and Parasites., Shahidi F and Botta, J.R., Eds., Seafoods Chemistry, Processing technology and Quality, Chapman & Hall, Glasgow., pp 220-232
  41. Wogu, M.D., Maduakol, M., 2010. Evaluation of Microbial Spoilage of some aqua cultured fresh fish in Benin city, Nigeria., Ethiopian J. Environ. Studies Manag., 3 (36): 18-22
 

[Mohamed Ibrahim M.A, SamiShabeeb Al Shabeeb, Ghamri H. Al Ramadhan and Peer Mohamed Imran. (2017); ISOLATION OF ENTEROBACTERIACEAE FROM RAW SEAFOODS SOLD IN FISH MARKETS IN EASTERN PROVINCE OF SAUDI ARABIA. Int. J. of Adv. Res. 5 (Mar). 1711-1718] (ISSN 2320-5407). www.journalijar.com


Mohamed Ibrahim M.A.
Fisheries Research Center, Deputy Ministry for Fisheries, Ministry of Environment, Water & Agriculture, Kingdom of Saudi Arabia.

DOI:


Article DOI: 10.21474/IJAR01/3683      
DOI URL: https://dx.doi.org/10.21474/IJAR01/3683