29Mar 2017

BIO DEGRADATION AND CYTOTOXIC POTENTIAL OF BIOSURFACTANT FROM MARINE BACTERIA ASSOCIATED WITH ALGAE ULVA LACTUCA.

  • Department of Biotechnology, Malankara catholic college, Mariagiri, Kaliakkavilai-629153, Kanya Kumari District, Tamil Nadu.
  • Head, Department of Biotechnology, Malankara catholic college, Mariagiri, Kaliakkavilai 629153, Kanya Kumari District, Tamil Nadu.
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Background: The use of biosurfactants to protect the marine environment seems possible since a number of marine bacterial strains can produce biosurfactants during growth on hydrocarbons (Bertrand et al., 1993). For the sake of the environment, the use of biosurfactants is preferable to those of synthetic surfactants. However, little information on either biosurfactant produced by marine microorganisms or biosurfactants active in saline has been reported so far. Objective: The isolated colonies were identified, potential biosurfactant producer was screened out. Biosurfactant extraction was done by lowering pH using 5M HCl followed by the detection and purification. The extracted biosurfactants were also used to study the effect on metal removal and anticancer activities. Materials and method: The antibacterial activity was carried out by disc diffusion method, screening of potential biosurfactant producers by hemolytic assay, drop collapsing test, oil displacement test and emulsification activity, detected and purified by silica gel plate (TLC). Anticancer activity was done by Dimethyl thiazolyl diphenyl tetrazolium bromide, (MTT). Effects on metal removal were studies using the media with salts of CrSO4 and ZnSO4. Result: Antimicrobial activity of SP2 showed highest activity against Pseudomonas aeruginosa (1cm) and Bacillus megaterium (2.2cm). Species 2 showed highest activity in the screening methods. TLC showed the presence of lipopeptide and rhamnolipid. The biodegradation using same concentration of bio surfactants produced by SP2, increased with increased concentration of the salts. SP2 microbial extracts showed cytotoxic activity in L929 cell line. These findings suggest that the identified sponges are source of pharmaceutical important compounds.

  1. Arimi S.M, Park R.W.A, Fricker C.R (1990): Study of hemolytic activity of some camphylobacter spp. On blood agar plates. J App. Bacteriol;69:384-389
  2. Arung E.T, Kusuma I.W, Ishandar Y.M, Yasutake S, Shimizu K, Kondo. R, (2000): Screening of medicinal plants Nat. Med., 63: 473-480.
  3. Banat I.M., Makkar R.S, Cameotra S.S. (2000): Potential commercial Application of Microbial Surfactants. Applied Microbiol Biotechnol.53:495-508.
  4. Bauer A.W, Kirby W.M.M, Sherries S.C, Turk. M (1996): Antibiotic susceptibility of testing by a standard single disc method. J.Clinical.Pathol:36:492-496.
  5. J (2005). Bioactive microbial metabolites. A personal view. Journal of Antibiotics. 43: 57-90
  6. Bergey D. H., Harrison, F. C., Breed, R. S., Hammer, B. W., and Huntoon, F. C (1994): Bergey’s manual of determinative bacteriology, 9th The Williams and Wilkins Co-publishers, Baltimore.
  7. Bertrand J.C, Bonin P, Goutx M, Mille G (1993): Biosurfactant production by marine microorganisms: potential application to fighting hydrocarbon marine pollution Mar. Biotechnol., 1: 125-129.
  8. Bodour A. A., and Miller-Maier, R. M (1998): Application of a modified drop-collapse technique for surfactant quantification and screening of biosurfactant- producing microorganism. Journal of Microbiological Methods, 32(3), 273-280.
  9. Cameotra S.S, Makkar R.S (1998): Synthesis of biosurfactants in extreme conditions. Appl Microbiol Biotechnol; 50:520–529.
  10. P., Mardaraz. C., Pitta Alvarez. S.I., and Giulietti. A.M (1996): Isolation and election biosurfactant-producing bacteria. World J. Microbiol. Biotechnol., 12: 82-84.
  11. Desai J.D and Banat I.M (1997): Microbial production of surfactants and their commercial potential. Mol. Biol. Rev., 61: 47-64.15
  12. Francesco Crescenzi, Mercell Camilli, Eugenio Fascetti, Filippo Parcelli, Giulio Prasperi, Pasquele Saccedder, (1971): Microbial degradation of biosurfactant dispersed oil. Journal of international oil spill., 111: 124-130
  13. Fricker C.R, Ale Mohammad M.M, Spencer S, Park R.W.A (1985): The application of new and established tests to characterize unusual camphylobacter isolated from men, animals and the environment. In: Pearson. A.D, Skirrow.M.B, Lior.H, Roweb (eds) Camphylobacter 3. London, Public Health Laboratory Service; 224-225.
  14. Georgiou G, Lin S.C, Sharma M.M, (1990): Surface active compounds from microorganisms Bio/ Technology 10: 60-65.
  15. Harayama S, Y Kasai & A Hara. (2004): Microbial communities in oil-contaminated seawater. Current Opinion in Biotechnology 15: 205-214
  16. Kingsley Urum and Turgay Pekdemir, (2004): Evaluation of biosurfactants for crude oil contaminated soil washing. Chemosphere 57: 1139-1150.
  17. Kitamoto D., Morita T., Fukuoka T., Konishi M-A., Imura T., (2009): Self-assembling properties of glycolipid biosurfactants and their potential applications. Op. Colloid Interface Sci. 14 (5). 315-328.
  18. M., Ito. M and Imanaka. T (1992): Isolation of a new surfactin producer Bacillus pumilus A-1, and cloning and nucleotide sequence of the regulator gene, psf-1. J. Ferment. Bioeng., 74: 255-261.
  19. R. M., Abalos. A., Gomez. J.M., Cantero. D., (2007): Biosorption of heavy metals by pseudomonas aerogenosa isolated from a petroleum contaminated site. Adv. Mater. Res. 20-21, 615-618.
  20. K, Gunkel. W, Lang. S, Wagner. F (1991): Marine biosurfactants, III. Toxicity testing with marine microorganisms and comparison with synthetic surfactants, Z. Naturforsch, 46c: 210- 216.
  21. Rashedi H., Assadi M.M., Bonakdarpour. B et al (2005): Environmental importance of rhamnolipid production from molasses as a carbon source. Int J Environ Sci Tech 2: 59-62.
  22. Rodrigues L.R, Teixeira J.A, Vander Mei. H.C, Oliveira R (2006): Physicochemical and functional characterization of a biosurfactant produced by Lactococcus lactis Colloids and Surfaces B: Biointerfaces; 49: 79–86.
  23. E.B., Gottleib. D., (1966): Internationl Journal of Systematic and Evolutionary Microbiology 16: 313-340.

[Geethu George and W.A. Manjusha (2017); BIO DEGRADATION AND CYTOTOXIC POTENTIAL OF BIOSURFACTANT FROM MARINE BACTERIA ASSOCIATED WITH ALGAE ULVA LACTUCA. Int. J. of Adv. Res. 5 (Mar). 1476-1482] (ISSN 2320-5407). www.journalijar.com

Dr. W.A. Manjusha
Head, Dept. of Biotechnology, Malankara Catholic College, Mariagiri, Kaliakkavilai, Tamil Nadu, India.

DOI:

Article DOI: 10.21474/IJAR01/3655      
DOI URL: https://dx.doi.org/10.21474/IJAR01/3655

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