28Feb 2017

PRODUCTION OF BIOPLASTICS FROM MICROORGANISMS.

  • Vijaya College, Department of Microbiology, R.V.Road, Basavangudi, Bangalore.
Crossref Cited-by Linking logo

Plum Print visual indicator of research metrics
  • Captures
    • Readers: 23
see details

  • Abstract
  • Keywords
  • References
  • Cite This Article as
  • Corresponding Author

The deleterious effects of synthetic plastics and their products have become a major concern for researchers. Bioplastics or plastics produced by the microorganism is a promising replacement for the conventional synthetic plastics. Polyhydroxyalkanoate a biologically produced biodegradable substance that has characteristic properties similar to that of conventional plastics. Polyhydroxyalkanoates are secondary metabolites of microorganisms which are produced under stressful conditions. In this work, four different samples were collected. These strains were then morphologically and biochemically characterized. The strains producing polyhydroxyalkanoates from each sample were identified by Sudan Black staining. A 48-hour culture of these strains was harvested and alkali lysis method was used to isolate polyhydroxyalkanoate and polyhydroxyalkanoate was quantified. Sample 2 had the highest polyhydroxyalkanoate accumulation % (95.65%). The method used for the production and isolation of polyhydroxyalkanoate was cost effective and ecofriendly .

  1. Amirul, A.,? Yahya,? A.R.M.,? Suresh,? K.,? Azizan,? M.N.M? and? Majid,? M.I.A. 2008.? Biosynthesis? of? poly? (3-hydroxy? butyrate-co-4-hydroxybutyrate)? copolymer? by Cupriavidussp.? USMAA1020? isolated? from? lake? Kulim? Malaysia.? Bioresour Technol.,? 99: 4903-4909
  2. BARTHOLOMEW, W.? (1962).? Variables? influencing? results? and? the? precise definition? of? steps? in? Gram? staining? as? a? means? of? standardising? the? results obtained.? Stain? Technology? 37,? 139-1 55.? v
  3. Burdon, L.,? Stokes,? J.C. Kimbrough,? C.E.1942.? Studies? of? the? common? aerobic spore? forming? bacilli? staining? for? fat? with? Sudan? Black? B? ?? stain.? Journal? of bacteriology, ?43,? 717 ? 724.
  4. CERNY, ? (1976).? A? method? for? the? distinction? of? Gram? negatives? from? Gram-positive? bacteria.? European? Journal? of? Applied? Microbiology? 3,? 223-225.
  5. Chen, Q.? A? microbial? polyhydroxyalkanoates? (PHA)? based? bio? and? materials industry.? Chem.? Soc.? Rev.? 2009;? 38,? 2434?2446
  6. Clarke, K. R..? A? simplified? plate? method? for? detecting? gelatin-liquefying? bacteria. J. Clin.? Pathol. 1953: 6:246-248.
  7. Du, ,? Chen,? J.,? Yu,? J? and? Lun,? S.? 2001.? Continuous? production? of? poly-3-hydroxybutyrate? by? Ralstonia? eutropha? in? a? two-stage? culture? system.? J.? Biotech., 88: 59-65
  8. Elbahloul Y,? Steinb?chel? A:? Large-scale? production? of? poly? (3-hydroxyoctanoic acid)? by? Pseudomonas? putida? GPo 1? and? a? simplified? downstream? ?Appl Environ? Microbiol? 2009,? 75: 643?651. 10.1128/AEM.01869-08
  9. GREGERSEN, ? (1978).? A? rapid? method? for? distinction? of? Gram-negative? from Gram-positive? bacteria.? European? Journal? of? Applied? Microbiology? and? Bio- technology? 5,? 123-1 27.
  10. Harley, P.? Laboratory? exercises? in? microbiology,? 6th? ed.? McGraw-Hill,? New York,? NY.2005.
  11. Hiss, H. 1897Jour.Exp.Med., 2,677.
  12. Hiss, H. 1902Jour.Med.Res.,8,148.
  13. http://microbeonline.com/colony-morphology-bacteria-describe- bacterial-colonies/
  14. http://shodhganga.inflibnet.ac.in/bitstream/10603/38487/7/chapter%203.pdf
  15. Isenberg D.,? and? L. H. Sundheim.? Indole? reactions? in? bacteria.? J.? Bacteriol. 1958; 75:682?690.
  16. Khanna S,? Srivastava? ? Recent? advances? in? microbial? polyhydroxyalkanoates. Process? Biochem.? 2005;? 40(2):607-619.
  17. Larke, ? H.,? and? S. T. Cowan.? Biochemical? methods? for? bacteriology.? J.? Gen.
  18. Lee, Y.? 1996.? Bacterial? polyhydroxyalkanoates.? Biotechnology? and? Bioengineering,? 49:? 1-14.? Microbiology? review,? 103,? 131-140
  19. LEVINE, MAX? 1916? Bact.,? 1,? 619.
  20. MacFaddin, F.? Biochemical? tests? for? identification? of? medical? bacteria,? 3rd? ed. Lippincott? Williams? &? Wilkins,? Philadelphia,? PA.2000.
  21. Michael Pelczar.,? ECS Chan,? Noel? R. Krieg.? McGraw? company? New? York? Fifth edition.? 1986:? 597.
  22. 1952:? 6:187-197.
  23. Ramsay JA,? Berger? E,? Ramsay? A,? Cherie? C:? Extraction? of? poly-3-hydroxybutyrate using? chlorinated? ?Biotechnol? Tech? 1994,? 8: ?589?594. 10.1007/BF00152152
  24. Yamanaka, ,? Kimura,? Y.,? Aoki,? T? and? Kudo,? T.? 2010.? Effect? of? ethylene? glycol on? the? end? group? structure? of? poly? (3-hydroxybutyrate).? Polym.Dead.? Stab.,? 95: 1284-1292.
  25. Zakaria, ? R.,? Ariffin,? H.,? Johar,? N.? A.? M.,?? Aziz,?? S.? A.,? Nishida,? H.,? Shirai,? Y and? Hassan,? M.? A.?? 2010.? Biosynthesis? and? characterization? of? poly? (3-hydroxybutyrate-co-3-hydroxybutyrate)? Copolymer? from? wild-type? Comamonas? sp. EB172.? Polym.Degrad.? Stab.,? 95:? 1382-1386.

[Sneha Bhat, Nichith K R, Kiran Y, Nagendra M, Pallavi S L, Shreya S, Pruthvi B and *Madhumita Ghosh Dastidar. (2017); PRODUCTION OF BIOPLASTICS FROM MICROORGANISMS. Int. J. of Adv. Res. 5 (Feb). 2710-2716] (ISSN 2320-5407). www.journalijar.com

Madhumita Ghosh Dastidar
Bangalore University

DOI:

Article DOI: 10.21474/IJAR01/3465      
DOI URL: https://dx.doi.org/10.21474/IJAR01/3465

Download Full Paper

Download PDF No. of Downloads: 1108 | No. of Views: 3429