28Feb 2017

EVALUATION OF ANTICANCER ACTIVITY OF SOME VENOMOUS ANIMAL TOXINS ON HUMAN BREAST AND COLON CANCER CELL LINES AND RELATED ANTIOXIDANT PROFILE

  • Department of Zoology, Faculty of girl for science and art, Ain Shams University, Naser City, Cairo, Egypt.
  • R&D Sector, The Holding Company for Production of Vaccines, Sera and Drugs (VACSERA), Dokky, Giza, Egypt.
  • Department of Zoology, Faculty of science, Professor of physiology and Director of Genetic Engineering Center, Al-Azhar University, Naser City, Cairo, Egypt.
  • Department of Zoology, Faculty of girl for science and art, Professor of Cytogenetic and Director of Zoology department, Ain Shams University, Naser City, Cairo, Egypt.
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Background:- Breast and colon cancers are the most types of cancer in Egyptian patients. Radiation and chemotherapy have undesirable side effects, so the drugs are used instead. Venoms have been used as folk medicine since ancient times. Purpose and aim:- This study examines the anticancer potential of Egyptian snake (Naja haje), scorpion (Leiurus quinquestriatus) and bee (Apis mellifera) venoms then compared to a synthetic anticancer drug 5-Flurouracil (5-FU). Methods:- The cytotoxicity activity of tested venoms was determined using SRB assay. Antioxidant potential was determined by the evaluation of the variation in MDA, NO. and GSH content. RNA was extracted to evaluate the expression of P53, Bcl2 and Bax genes using semi-quantitative RT-PCR analysis after 24hrs. treatment. Results & Discussion:- MCF-7 cells were more sensitive than Caco-2 cells to tested venoms while 5-FU was more toxic to both cell lines. The Naja h., Leiurus q. and Apis m. venoms had IC50 as 3.5, 27.8 and 2.5 µg/ml with MCF-7 cell line and 20, 32.3 and 14 µg/ml with Caco-2 cell line respectively. The Naja h. venom had apoptotic effect against MCF-7 and Caco-2 cell lines through the elevation of p53 and depletion of Bcl-2 gene expression with increasing NO. and decreasing in GSH contents. The Leiurus q. venom had apoptotic effect against MCF-7 and Caco-2 cell lines through the elevation of Bax and depletion of Bcl-2 gene expression with elevation in NO. level. The Apis m. venom had apoptotic effect against MCF-7 and Caco-2 cell lines through the elevation of p53 and Bax with depletion of Bcl-2 gene expression and increasing NO. and decreasing in GSH contents. While 5-FU was toxic through depletion of Bcl-2 gene expression and increasing NO. level. Conclusion:- Finally, it can be concluded that snake (Naja h.), scorpion (Leiurus q.) and bee (Apis m.) venoms had anticancer potentials on human breast and colon cancer cells and this is positively related to the antioxidant profile and apoptotic gene expression.

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[Abdel-Aziz S. A. A, Mohamed A. F, Zahkouk S. A. M and Ali R. A. M. (2017); EVALUATION OF ANTICANCER ACTIVITY OF SOME VENOMOUS ANIMAL TOXINS ON HUMAN BREAST AND COLON CANCER CELL LINES AND RELATED ANTIOXIDANT PROFILE Int. J. of Adv. Res. 5 (Feb). 2036-2053] (ISSN 2320-5407). www.journalijar.com

Salma A. A. Abdel-Aziz
Faculty of girl for science and art, Ain Shams University, Naser City, Cairo, Egypt.

DOI:

Article DOI: 10.21474/IJAR01/3376      
DOI URL: https://dx.doi.org/10.21474/IJAR01/3376

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