EVALUATION OF CANDIDA SPECIES FROM CLINICAL SPECIMENS BY USING CHROMAGAR.
- Ph. D Scholar, Jaipur National University.
- Director, Life Sciences , Jaipur National University.
- Abstract
- Keywords
- References
- Cite This Article as
- Corresponding Author
Candida species especially Non albicans Candida are increasingly being isolated from clinical specimens. Candida spp. are the most common cause of fungal infections and are the fourth leading cause of health care associated infections. The main aim of the study is to isolate and identify the various Candida species from clinical samples by both Conventional and chromgenic method and to check which one is better and specific method. Samples such as blood, urine and pus received in laboratory from patients of all age group and both sexes with suspected Candida infection and the positive isolates were identified by conventional as well as chromogenic method. Total 80 isolates of Candida species were recovered from the clinical specimens. C.krusei was most commonly isolated yeast followed by C.albicans. CHROMagar falsely identified C.parapsilosis as C.glabrata. Species identification using CHROMagar is rapid, technically simple, and easy as compared to conventional method.
- Shivprakasha S, Radhakrishan K, Karim PMS. Candida spp other than Candida albicans. A major cause of fungemia in a tertiary care centre. Ind J Med Microbiol 2007;25(4):405-407.
- Bailey and Scotts, diagnostic microbiology, thirteenth edition, chapter 63 Mycology.
- Pahwa N, Kumar R, Nirkhiwale S, Bandi A. Species distribution and drug susceptibility of Candida in clinical isolates from a tertiary care centre at Indore. Indian J Med Microbiol 2014; 32(1): 44–8.
- Amy, R. (2000). Current issues in women's health. Controlling yeast
- Mohandas V, Ballal M. Distribution of Candida Species in different clinical samples and their virulence: Bio-film formation, proteinase and phospholipase production: A study on hospitalized patients in Southern India. J Glob Infect Dis 2011; 3: 4–8.
- Manjunath V, Vidya GS, Sharma A, Prakash MR, Murugesh. Speciation of Candida by Hichrom agar and Sugar assimilation test in both HIV infected and non infected patients. Int J Biol Med Res.2012;3(2):1778-1782.
- Dharwad S, Dominic S. Species identification of Candida isolates in various clinical specimens with their anti- fungal susceptibility patterns. J Clin diagnostic Res 2011; 5(6): 1177–81
- Deorukhkar S, Saini S. Non albicans Candida species: its isolation pattern, species distribution, virulence factors and antifungal susceptibility profile. Int J Med Sci Public Heal 2013; 2(3): 533–8.
- Baradkar VP, Mathur M, Kumar S. Hichrom candida agar for identification of Candida species. Indian J Pathol Microbiol 2010; 53(1): 93–5.
- Madhavan P, Jamal F, Chong PP, Ng KP. Identification of local clinical Candida isolates using CHROMagar Candida™ as a primary identification method for various Candida species. Trop Biomed 2011; 28(2): 269–74.
- Devi LS, Maheshwari M. Speciation of Candida Species Isolated From Clinical Specimens by Using Chrom Agar and Conventional Methods. Int J Sci Res Publ 2014;4(3): 1-5.
- Shettar SK, Patil AB, Nadagir SD, Shepur TA, Mythri BA, Gadadavar S. Evaluation of HiCrome differential agar for speciation of Candida. J Acad Med Sci 2012; 2(3): 101–4
- Oxoid Ltd.Oxoid brilliance candida agar [pamphlet].Oxoid Ltd;UK.
[Preeti Sharma, Sorabh Singh Sambyal and Divya Shrivastava. (2017); EVALUATION OF CANDIDA SPECIES FROM CLINICAL SPECIMENS BY USING CHROMAGAR. Int. J. of Adv. Res. 5 (Feb). 1750-1755] (ISSN 2320-5407). www.journalijar.com
Jaipur National University