30Jan 2017

PHENOTYPIC DETECTION OF BIOFILMS IN CANDIDA SPECIES ISOLATED FROM VARIOUS CLINICAL SPECIMEN

  • Ph.D Scholar, Jaipur National University.
  • Director, Life Sciences, Jaipur National University.
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Candida is one of the most frequently encountered opportunistic fungi that cause severe infection in humans because of its virulence factor. The ability of Candida albicans to form biofilms and adhere to host tissues and biomaterial surfaces is an important factor in its pathogenesis. One of the main characteristics of biofilms is their resistant to broad spectrum anti-microbial drugs. The aim of the study was to know the biofilm formation by various Candida species isolated from various clinical specimens. The study was carried out over a period of 1 year from January 2016 to December 2016 at the Department of Microbiology, Government Medical College and Hospital, Jammu. A total 120 Candida spp. were isolated from various clinical specimens. Speciation of Candida was done by standard yeast identification protocol and Candida CHROMagar. Biofilm formation was detected by Congo Red Agar, Tube method and microtitre plate method. Out of total 120 Candida spp. studied, biofilm production was seen in 63/100 (52.55%) isolates. While comparing all the three methods tube method proved more reliable, easy and more efficient. Antifungal efficacy of Coconut oil and Eucalyptus oil was also tested in this study against all Candida isolates. Eucalyptus oil was observed to be a better antifungal agent than Coconut oil in the present study. When coconut oil was tested against all Candida albicans isolates, the sensitivity of biofilm non producers was higher in comparison to biofilm producers.

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[Preeti Sharma, Sorabh Singh Sambyal and Divya Shrivastava. (2017); PHENOTYPIC DETECTION OF BIOFILMS IN CANDIDA SPECIES ISOLATED FROM VARIOUS CLINICAL SPECIMEN Int. J. of Adv. Res. 5 (Jan). 2017-2023] (ISSN 2320-5407). www.journalijar.com

Preeti Sharma
Ph.D Scholar, Jaipur National University

DOI:

Article DOI: 10.21474/IJAR01/2973      
DOI URL: https://dx.doi.org/10.21474/IJAR01/2973

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