30Jan 2017

PHENOTYPIC AND MOLECULAR ANALYSIS IN TINOSPORA CORDIFOLIA.

  • Department of Botany, Sant gadge baba amravati university, Amravati-444602
  • Abstract
  • Keywords
  • References
  • Cite This Article as
  • Corresponding Author

Natural products with medicinal value are gradually gaining importance in clinical research due to their well-known property of no side effects as compared to drugs. Tinospora cordifolia commonly named as “Guduchi” is known for its immense application in the treatment of various diseases in the traditional ayurvedic literature. Recently the discovery of active components from the plant and their biological function in disease control has led to active interest in the plant across the globe. Our present study in this review encompasses (i) the genetic diversity of the plant and (ii) active componen ts isolated from the plant and their biological role in disease targeting. The future scope of the review remains in exploiting the biochemical and signaling pathways affected by the compounds isolated from Tinospora so as to enable new and effective formulation in disease eradication.


  1. Upadhyay A.K., Kumar K., Kumar A., Mishra H.S., Tinospora cordifolia (Willd.) Hook. f. and Thomas. 2010.,(Guduchi)-validation of the Ayurvedic pharmacology through experimental and clinical studies. Int J Ayurveda Res.;1:112–21
  2. Rout G.R. 2006., Identification of Tinospora cordifolia (Willd.) Miers ex Hook F & Thomas using RAPD markers. Z Naturforsch C.;61:118–22.
  3. Sharma U., Bala M., Kumar N., Singh B., Munshi R.K., Bhalerao S.2012., Immunomodulatory active compounds from Tinospora cordifolia. J Ethnopharmacol.;141:918–26.
  4. Karpova E.A., Voznyi Y. V., Dudukina T.V., Tsvetkova I.V. 1991., 4-Trifluoromethylumbelliferyl glycosides as new substrates for revealing diseases connected with hereditary deficiency of lysosome glycosidases. Biochem Int.;24:1135–44.
  5. Kapil A., Sharma S. 1997., Immunopotentiating compounds from Tinospora cordifolia. J Ethnopharmacol.;58:89–95.
  6. Swaminathan K., Sinha U.C., Bhatt R.K., Sabata B.K., Tavale S.S. 1989;Structure of tinosporide, a diterpenoid furanolactone from Tinospora cordifolia Acta Crystallogr C. 45:134–6.
  7. Sundarraj S., Thangam R., Sreevani V., Kaveri K., Gunasekaran P., Achiraman S., 2012., et al. ?-Sitosterol from acacia nilotica induces G2/M cell cycle arrest and apoptosis through c-Myc suppression in MCF-7 and A549 cells. J Ethnopharmacol.;141:803–9.
  8. Thippeswamy G., Sheela M.L., Salimath B.P. 2008., Octacosanol isolated from Tinospora cordifolia downregulates VEGF gene expression by inhibiting nuclear translocation of NF-<kappa>B and its DNA binding activity. Eur J Pharmacol.;588:141–50.
  9. Ghosh A.K., Chapsal B.D., Weber I.T., Mitsuya H. 2008., Design of HIV protease inhibitors targeting protein backbone: An effective strategy for combating drug resistance. Acc Chem Res.;41:78–86.
  10. Tripathi Y.B., Sharma M., Manickam M. 1997., Rubia 5 din, a new antioxidant from rubia cordifolia. Indian J Biochem Biophys.;34:302–6.
  11. More P., Pai K. 2012.,In vitro NADH-oxidase, NADPH-oxidase and myeloperoxidase activity of macrophages after Tinospora cordifolia (guduchi) treatment. Immunopharmacol Immunotoxicol.;34:368–72.
  12. Upadhyaya R., P.R., Sharma V., Anita K.V., 2011.,Assessment of the multifaceted immunomodulatory potential of the aqueous extract of Tinospora cordifolia. Res J Chem Sci.;1:71–9.
  13. Sudhakaran D.S., Srirekha P., Devasree L.D., Premsingh S., Michael R.D. 2006., Immunostimulatory effect of Tinospora cordifolia Miers leaf extract in Oreochromis mossambicus. Indian J Exp Biol.;44:726–32.
  14. Raghu R., Sharma D., Ramakrishnan R., Khanam S., Chintalwar G.J., Sainis K.B., 2009., Molecular events in the activation of B cells and macrophages by a non-microbial TLR4 agonist, G1-4A from Tinospora cordifolia. Immunol Lett.;123:60–71.
  15. Shivananjappa M.M., 2012., Muralidhara Abrogation of maternal and fetal oxidative stress in the streptozotocin-induced diabetic rat by dietary supplements of Tinospora cordifolia. Nutrition.;28:581–7.
  16. Gupta R., Sharma V. 2011.,Ameliorative effects of Tinospora cordifolia root extract on histopathological and biochemical changes induced by aflatoxin-b (1) in mice kidney. Toxicol Int.;18:94–8.
  17. Kapur P., Wuttke W., Jarry H., Seidlova-Wuttke D. 2010., Beneficial effects of beta-Ecdysone on the joint, epiphyseal cartilage tissue and trabecular bone in ovariectomized rats. Phytomedicine.;17:350–5.
  18. Akhtar S., 2010.,Use of Tinospora cordifolia in HIV infection. Indian J Pharmacol.;42:57.
  19. Rao S.K., Rao P.S., 2010 Alteration in the radiosensitivity of HeLa cells by dichloromethane extract of guduchi (Tinospora cordifolia) Integr Cancer Ther. ;9:378–84.
  20. Chaudhary R., Jahan S., Goyal P.K. 2008., Chemopreventive potential of an Indian medicinal plant (Tinospora cordifolia) on skin carcinogenesis in mice. J Environ Pathol Toxicol Oncol.;27:233–43.
  21. Leyon P.V., Kuttan G. 2004 Inhibitory effect of a polysaccharide from Tinospora cordifolia on experimental metastasis. J Ethnopharmacol.;90:233–7.
  22. Diwanay S, Chitre D, Patwardhan B. 2004., Immunoprotection by botanical drugs in cancer chemotherapy. J Ethnopharmacol.;90:49–55.
  23. Jagetia G.C, Nayak V., Vidyasagar M.S. 1998 Evaluation of the antineoplastic activity of guduchi (Tinospora cordifolia) in cultured HeLa cells. Cancer Lett.; 127:71–82.
  24. Narayanan A.S., Raja S.S., Ponmurugan K., Kandekar S.C., Natarajaseenivasan K., Maripandi A.2011., et al. Antibacterial activity of selected medicinal plants against multiple antibiotic resistant uropathogens: A study from Kolli Hills, Tamil Nadu, India. Benef Microbes.; 2:235–43.
  25. Jeyachandran R., Xavier T.F., Anand S.P. 2003., Antibacterial activity of stem extracts of Tinospora cordifolia (Willd) Hook. f and Thomson. Anc Sci Life.; 23:40–3.
  26. Tambekar D.H., Khante B.S., Chandak B.R., Titare A.S., Boralkar S.S., Aghadte S.N.2009., Screening of antibacterial potentials of some medicinal plants from Melghat forest in India. Afr J Tradit Complement Altern Med.; 6:228–32.
  27. Thatte U.M., Kulkarni M.R., Dahanukar S.A. 1992, Immunotherapeutic modification of Escherichia coli peritonitis and bacteremia by Tinospora cordifolia. J Postgrad Med.; 38:13–5
  28. Sengupta M., Sharma G.D., Chakraborty B. 2011., Effect of aqueous extract of Tinospora cordifolia on functions of peritoneal macrophages isolated from CCl 4 intoxicated male albino mice. BMC Complement Altern Med.; 11:102.
  29. Purandare H, Supe A. 2007.,Immunomodulatory role of Tinospora cordifolia as an adjuvant in surgical treatment of diabetic foot ulcers: A prospective randomized controlled study. Indian J Med Sci.; 61:347–55.
  30. Sivakumar V., Rajan M.S. 2010. Antioxidant Effect of Tinospora cordifolia Extract in Alloxan-induced Diabetic Rats. Indian J Pharm Sci.; 72:795–8
  31. Prince Stanely Mainzen P., Menon V.P. 2001, Antioxidant action of Tinospora cordifolia root extract in alloxan diabetic rats. Phytother Res.; 15:213–8.
  32. Gacche R.N., Dhole N.A., 2011. Profile of aldose reductase inhibition, anti-cataract and free radical scavenging activity of selected medicinal plants: An attempt to standardize the botanicals for amelioration of diabetes complications. Food Chem Toxicol.; 49:1806–13.
  33. Rawal A., Muddeshwar M., Biswas S., 2004 Effect of rubia cordifolia, Fagonia cretica linn, and Tinospora cordifolia on free radical generation and lipid peroxidation during oxygen-glucose deprivation in rat hippocampal slices. Biochem Biophys Res Commun.; 324:588–96.
  34. Mathew S., Kuttan G., 1997 Antioxidant activity of Tinospora cordifolia and its usefulness in the amelioration of cyclophosphamide induced toxicity. J Exp Clin Cancer Res.;16:407–11.
  35. Khan MI., Harsha PS., Giridhar P, Ravishankar G. A.2011 Pigment identification, antioxidant activity, and nutrient composition of Tinospora cordifolia (willd.) Miers ex Hook. f and Thomas fruit. Int J Food Sci Nutr. ;62:239–49.

[Nutanvarsha Deshmukh. (2017); PHENOTYPIC AND MOLECULAR ANALYSIS IN TINOSPORA CORDIFOLIA. Int. J. of Adv. Res. 5 (Jan). 1990-1998] (ISSN 2320-5407). www.journalijar.com


MISS NUTANVARSHA PRADIPRAO DESHMUKH
Department of Botany, Sant gadge baba amravati university, Amravati-444602

DOI:


Article DOI: 10.21474/IJAR01/2970      
DOI URL: https://dx.doi.org/10.21474/IJAR01/2970