20Jan 2017

VITAMIN D3 IMPROVES LIVER FUNCTIONS IN EXPERIMENTALLY INDUCED TYPE II DIABETES MELLITUS IN WISTAR RATS

  • Lecturer of Physiology, Faculty of Medicine, Ain Shams University.
  • Lecturer of Clinical Pathology, Faculty of Medicine, Ain Shams University Cairo, Egypt.
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Background and Aim: Vitamin D3 could ameliorate the effects of type 2 diabetes mellitus; however, liver changes in diabetes needs more studies and to highlight changes in liver cell mitochondrial NAD+ and Stearoyl-CoA desaturase 1 (SCD1). Materials and Methods: Forty adult Wistar rats, of both sexes, were randomly allocated into equal groups of control, vitamin D3 non-diabetic, diabetic, type 2 diabetic and vitamin D3 treated diabetic groups. At the end of the study, all rats were anaesthetized by i.p. Pentoparbitone (40 mg/kg B.W). Fasting blood glucose level was measured by rat tail, then after aortic cannulation, the separated plasma was used for determination of lipid profile, liver functions and insulin level. HOMA-IR and atherogenic indices were calculated. Liver specimens were used for detection of SCD1 gene expression by Real time PCR and mitochondrial NAD+. Results: Glycemic parameters and plasma liver enzymes, liver mitochondrial NAD+ were significantly elevated in the diabetic group compared to the control group, and were significantly lowered in vitamin D3 treated diabetic group compared to the diabetic group. The diabetic group had dyslipidemia compared to the control group, while lipid profile was improved in vitamin D3 treated diabetic group compared to the diabetic group. SCD1 gene expression in liver cells was downregulated in both diabetic groups (untreated and treated) compared to the control group. Conclusion: Vitamin D3 reversed the damaging diabetic effects on liver functions efficiently, probably by ameliorating oxidative stress, however, it altered SCD1 gene expression and regulated its effect on diabetes to lesser extent.

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[Noha N. Lasheen and Nashwa El-Khazragy. (2017); VITAMIN D3 IMPROVES LIVER FUNCTIONS IN EXPERIMENTALLY INDUCED TYPE II DIABETES MELLITUS IN WISTAR RATS Int. J. of Adv. Res. 5 (Jan). 1715-1726] (ISSN 2320-5407). www.journalijar.com

NOHA N. LASHEEN
Lecturer of Physiology, Faculty of Medicine, Ain Shams University

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Article DOI: 10.21474/IJAR01/2942      
DOI URL: https://dx.doi.org/10.21474/IJAR01/2942

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