CREATION OF NOVEL RESTRICTION SITES IN VIVO IN CHICK EMBRYO NEURON-DNA AFTER ACRYLAMIDE TREATMENT-RESTRICTION MAPING ANALYSIS FOR Bam HI AND Hind III RESTRICTION ENDONUCLEASES

The genotoxicity of acrylamide (AC) with a significantly high chemical activity can cause many adverse effects to health of an organism. The present study investigation has analyzed the effect of acrylamide on DNA modification to insert new fragmentation sites on prolonged administration of cytotoxicity. The spectrophotometric quantification of DNA after increased administration of acrylamide to neurons shows that the amounts of DNA decrease in neurons. To test the insertion of new sites on DNA by acrylamide DNA fragment having 13.2kb length was electroeluted from the agarose gel from control and treated, and digested with Hind III and Bam HI restriction enzymes to generates restriction maps on agarose electrophoresis, and the restriction maps were analyzed by comparing with control. The acrylamide treatment has shown (0.5 and 0.6mg) significantly induced DNA damage through the formation of additional restriction site for the Bam HI at 31 end of the neuron-DNA. From the results of the present study it was observed that the digestion of the DNA fragments from control and treated have produced unique banding pattern for each sample. It showed that the degree of the DNA damage was dose dependent.