Molecular identification of Toxoplasma gondii from domestic cats in Diwanyia city.
- College of Science, University of Al-Qadissiya, Al-Diwaniya, IRAQ.
- College of Veterinary Medicine, University of Al-Qadissiya, Al-Diwanyia, IRAQ.
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The aim of this study was to determine the occurrence Toxoplasma gondii infection in domestic cats using Immunoassay Rapid IgM /IgG test and polymerase chain reaction (PCR). The blood samples were collected from 96 cats from Diwanyia city. Then all samples were investigated by Immunoassay to distinguish of chronic and acute or reactivated infections Toxoplasma infection and only positive IgM or IgG cases were subjected to polymerase chain reaction. PCR was based on used specific primers that amplification of 529bpfragment in DNA of Toxoplasma gondii. The serological survey results were show 58.3% (56/96) of IgG seropositive as chronic infection and 39.9% (38/96) IgM seropositive as reactivated chronic infection. PCR technique results show detection of Toxoplasma gondii in positive at (24%) and (23/96) respectively. We conclude that these study demonstrate that the direct Polymerase Chain Reaction (PCR) assay techniques is a simple, rapid and valuable tool for the detection of T. gondii in domestic cats, and these animals may be play important role that increase the risk of transmission of disease to human.
[Firas Hachim Naser, Hassan Hachim Naser and Najim Abd Al- waheid Al - Jadoa. (2016); Molecular identification of Toxoplasma gondii from domestic cats in Diwanyia city. Int. J. of Adv. Res. 4 (Sep). 964-968] (ISSN 2320-5407). www.journalijar.com
